Preparation of enrofloxacin nanoemulsion and evaluation of pharmacodynamic of spray administration

XU Ying; QIU Yangyang; SHEN Yue; FENG Huixin; FENG Yunyun; HUANG Xianhui

doi:10.7671/j.issn.1001-411X.202001030

Journal of South China Agricultural University > 2021 > 42(1): 42-48. > DOI: 10.7671/j.issn.1001-411X.202001030

XU Ying, QIU Yangyang, SHEN Yue, et al. Preparation of enrofloxacin nanoemulsion and evaluation of pharmacodynamic of spray administration[J]. Journal of South China Agricultural University, 2021, 42(1): 42-48. DOI: 10.7671/j.issn.1001-411X.202001030

Citation:

PDF (927 KB)

Preparation of enrofloxacin nanoemulsion and evaluation of pharmacodynamic of spray administration

College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China

More Information

Received Date: January 16, 2020
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To develop a new formulation of enrofloxacin nanoemulsion for spray administration, and evaluate its disease control effect using chicken model of systemic infection.

Method
The phase transition method was used, and single factor and orthogonal tests were performed to screen formulations and optimize the prescription. High-performance liquid chromatography (HPLC) was used to determine enrofloxacin content in nanoemulsion. Nanoparticle and zeta potential analyzer was used to measure the particle size and zeta potential of nanoemulsion. Pharmacodynamic experiments were performed using chicken models infected by Salmonella pullorum and Escherichia coli, and the control effects of spray adminstration of compound enrofloxacin nanoemulsion on bacterial diseases were evaluated.

Result
The nanoemulsion prepared in this experiment was an oil-in-water type, light yellow, clear and transparent, and had uniform droplet distribution. The average particle size was (10.79 ± 1.5) nm, the polydispersity index (PDI) was 0.446 and the zeta potential was −21.63 mV. The pharmacodynamic evaluation showed that the survival rates of preventive administration groups with high, medium and low doses were 90.0%, 83.3% and 73.3% respectively, which were significantly higher than that of the infection control group (26.7%) ( P<0.01).

Conclusion
The compound enrofloxacin nanoemulsion prepared in this study has stable properties. The ultrasonic spray administration has good control effects on chicken diseases due to systemic infection of Salmonella pullorum and Escherichia coli.
- enrofloxacin,
- nanoemulsion,
- spray administration,
- poultry,
- Salmonella pullorum,
- Escherichia coli

FullText(HTML)

References (27)

References

[1]	SAIF Y M, 主编. 禽病学: 12版[M]. 苏敬良, 高福, 索勋, 译. 北京: 中国农业出版社, 2012: 811-870.
[2]	崔福德, 主编. 药剂学: 6版[M]. 北京: 人民卫生出版社, 2010: 201-213.
[3]	CALDERON-NIEVA D, GOONEWARDENE K B, GOMIS S, et al. Veterinary vaccine nanotechnology: Pulmonary and nasal delivery in livestock animals[J]. Drug Deliv Transl Re, 2017, 7(4): 558-570. doi: 10.1007/s13346-017-0400-9
[4]	VARGA M. Therapeutics[M]//Textbook of Rabbit Medicine: 2nd ed. Oxford: Butterworth-Heinemann, 2014: 137-177.
[5]	PERRY S M, MITCHELL M A. Antibiotic therapy[M]//Mader's reptile and amphibian medicine and surgery: 2nd Edition. Philadelphia: Saunders, 2019: 1139-1154.
[6]	陈杖榴. 兽医药理学: 3版[M]. 北京: 中国农业出版社, 2004.
[7]	MARTINEZ M, MCDERMOTT P, WALKER R. Pharmacology of the fluoroquinolones: A perspective for the use in domestic animals[J]. The Veterinary Journal, 2006, 172(1): 10-28. doi: 10.1016/j.tvjl.2005.07.010
[8]	方炳虎, 曾振灵, 冯淇辉, 等. 恩诺沙星对鸡大肠杆菌病及葡萄球菌病的药效研究[J]. 中国兽医学报, 1997(2): 54-57.
[9]	GAUTIER-BOUCHARDON A V, REINHARDT A K, KOBISCH M, et al. In vitro development of resistance to enrofloxacin, erythromycin, tylosin, tiamulin and oxytetracycline in Mycoplasma gallisepticum, Mycoplasma iowae and Mycoplasma synoviae[J]. Veterinary Microbiology, 2002, 88(1): 47-58. doi: 10.1016/S0378-1135(02)00087-1
[10]	SCHRODER J. Enrofloxacin: A new antimicrobial agent[J]. Journal of the South African Veterinary Association, 1989, 60(2): 122-124.
[11]	FLECKENSTEIN E, UPHOFF C C, DREXLER H G. Effective treatment of Mycoplasma contamination in cell lines with enrofloxacin (Baytril)[J]. Leukemia, 1994, 8(8): 1424-1434.
[12]	BAUDITZ R. Results of clinical studies with Bytril in poultry[J]. Veterinary Medical Review, 1987, 2: 130-136.
[13]	SUMANO L H, OCAMPO C L, BRUMBAUGH G W, et al. Effectiveness of two fluoroquinolones for the treatment of chronic respiratory disease outbreak in broilers[J]. British Poultry Science, 1998, 39(1): 42-46. doi: 10.1080/00071669889376
[14]	王波臻, 欧阳五庆, 纪俊明, 等. 复方恩诺沙星纳米乳的制备及体外透皮性能考察[J]. 畜牧与兽医, 2016, 48(3): 77-83.
[15]	毛庆祥, 常文军, 蔡全才. 桉叶油透皮吸收促进剂研究进展[J]. 药学实践杂志, 2003(4): 205-209. doi: 10.3969/j.issn.1006-0111.2003.04.005
[16]	DHAKAD A K, PANDEY V V, BEG S, et al. Biological, medicinal and toxicological significance of Eucalyptus leaf essential oil: A review[J]. Journal of the Science of Food and Agriculture, 2018, 98(3): 833-848. doi: 10.1002/jsfa.8600
[17]	朱向波. 鸡白痢沙门氏菌病诊断与防治[J]. 中国畜禽种业, 2015, 11(5): 149-150. doi: 10.3969/j.issn.1673-4556.2015.05.112
[18]	郑志新. 张家口地区鸡白痢沙门氏菌的分离鉴定及药敏试验[J]. 河北北方学院学报(自然科学版), 2015, 31(3): 36-38.
[19]	安辉, 张大华, 陈小玲, 等. 鸡白痢沙门氏菌的分离与鉴定[J]. 安徽农业科学, 2011, 39(32): 19888-19890. doi: 10.3969/j.issn.0517-6611.2011.32.091
[20]	刘吉山, 沈志强, 王燕, 等. 我国部分地区禽致病性大肠杆菌的分离鉴定与药敏试验[J]. 中国家禽, 2005(7): 14-16. doi: 10.3969/j.issn.1004-6364.2005.07.004
[21]	路卫星. 鸡大肠杆菌病的诊断与治疗[J]. 中国兽医杂志, 2015, 51(8): 43-44. doi: 10.3969/j.issn.0529-6005.2015.08.015
[22]	陈洪科. 禽病诊断和防治: 1版[M]. 北京: 中国农业科技出版社, 1996.
[23]	刘慕欣, 付领新. 喷雾给药治疗禽类呼吸道疾病的效果观察[J]. 当代畜牧, 2014(8): 76-77.
[24]	DAS A, AHMED A B. Natural permeation enhancer for transdermal drug delivery system and permeation evaluation: A review[J]. Asian Journal of Pharmaceutical and Clinical Research, 2017, 10(9): 5. doi: 10.22159/ajpcr.2017.v10i9.19389
[25]	陈宇. 桉叶挥发油制剂开发及其抗慢性支气管炎的药效研究[D]. 天津: 天津大学, 2008.
[26]	SADLON A E, LAMSON D W. Immune-modifying and antimicrobial effects of Eucalyptus oil and simple inhalation devices[J]. Alternative Medicine Review: A Journal of Clinical Therapeutic, 2010, 15(1): 33.
[27]	欧阳五庆, 杨增岐, 李雅. 恩诺沙星对人工诱发的鸡大肠杆菌病的疗效[J]. 西北农业大学学报, 1998: 76-79.

[1]	WU Caixia, LIU Zhaoming, YAN Quanmei, ZHANG Quanjun, OUYANG Zhen, ZHAO Yu, FAN Nana, LAI Liangxue. Gene knockout of Tiki1 gene in rabbit by TALEN system[J]. Journal of South China Agricultural University, 2020, 41(3): 9-14. DOI: 10.7671/j.issn.1001-411X.201908011
[2]	XIANG Zhenggang, GENG Yi, ZHANG Yuwei, YANG Zexiao, OU YANG Ping, LI Yajun, MOU Weihao, WANG Shizhen. Virulence detection and molecular typing of Staphylococcus aureus isolated from rabbit in Sichuan area[J]. Journal of South China Agricultural University, 2017, 38(4): 62-68. DOI: 10.7671/j.issn.1001-411X.2017.04.011
[3]	HU Shuaishuai, WANG Wenzhou, YAN Xiaorong, ZHAO Bohao, ZHU Jie, HAO Ye, CHEN Yang, WU Xinsheng. Myh6 gene expression and its correlation with muscle fiber traits of New Zealand white rabbits[J]. Journal of South China Agricultural University, 2017, 38(2): 12-17. DOI: 10.7671/j.issn.1001-411X.2017.02.003
[4]	YANG Dexue, CHEN Liangzhu, LAI Lihong, REN Miaoxian, ZHANG Guijun, PAN Zhikun, FANG Binghu. Research on pharmacokinetics and bioavailability of pleuromutilin derivative BC-7013 in chickens[J]. Journal of South China Agricultural University, 2015, 36(4): 26-31. DOI: 10.7671/j.issn.1001-411X.2015.04.005
[5]	TIAN Miaomiao, GUO Chunna, HUAI Binbin, GAO Jin, HUANG Xianhui. Studies on the excretion of robenidine hydrochloride in rabbit urine and feces[J]. Journal of South China Agricultural University, 2015, 36(3): 8-12. DOI: 10.7671/j.issn.1001-411X.2015.03.002
[6]	WANG Hua, ZHOU Xiaoqiong. The Effect of Injectio Xiangdan on Endotoxin-Induced Renal DIC in Rabbits[J]. Journal of South China Agricultural University, 2013, 34(2): 258-261. DOI: 10.7671/j.issn.1001-411X.2013.02.025
[7]	WANG Ang, DING Huan-zhong, GAO Yan, GAO Guo-feng, ZENG Zhen-ling. Pharmacokinetics and Bioavailability of Terbinafine Hydrochloride Capsules in Beagle Dogs[J]. Journal of South China Agricultural University, 2012, 33(4): 556-560. DOI: 10.7671/j.issn.1001-411X.2012.04.025
[8]	ZOU Meng-jia, LI Ke, CHEN Chao, SUN Yong-xue. Pharmacokinetics and Bioavailability of Roxarsone in Chickens[J]. Journal of South China Agricultural University, 2012, 33(3): 403-406. DOI: 10.7671/j.issn.1001-411X.2012.03.026
[9]	LI Xia, LIU Ai-shan, XU Lai-xiang, ZHAO Ya-hua. Tandem Expression of Rabbit Defensin Gene in Pichia pastoris and Tentative Study on the Antibacterial[J]. Journal of South China Agricultural University, 2011, 32(4): 57-62. DOI: 10.7671/j.issn.1001-411X.2011.04.013
[10]	Pharmacokinetics and Bioavailability of Doxycycline Injection in Pigs[J]. Journal of South China Agricultural University, 2010, 31(4). DOI: 10.7671/j.issn.1001-411X.2010.04.027

Cited By

Cited by

Periodical cited type(6)

1.	孙溱博，廖森泰，杨琼，刘吉平，肖阳，李庆荣，邢东旭. 热水浴法解除蚕卵滞育的条件优化及应用效果分析. 南方农业学报. 2024(01): 280-289 .
2.	孙溱博，刘吉平，廖森泰，杨琼，肖阳，李庆荣，邢东旭. 家蚕卵滞育人工解除方法及机制研究进展. 蚕业科学. 2024(02): 175-187 .
3.	龚竞，张伟，唐苗，程悦静，庞家欣. 家蚕小分子热激蛋白BmHsp19.1基因克隆及在蚕卵中的表达特征. 西南大学学报(自然科学版). 2023(06): 109-115 .
4.	崔春光，陈金娥，杜鑫，王永强，徐霞. 家蚕蚕卵孵化率影响因素的研究进展. 蚕桑通报. 2023(02): 1-5+22 .
5.	吴灵芝，宋璐，余天成，李保珍，樊金华. 不同球孢白僵菌菌株对家蚕的致病力分析. 山西农业科学. 2021(04): 504-508 .
6.	龚竞，杨领振，郑茜，唐苗，颜瑞丰，朱勇. 高浓度氧或HCl处理条件下家蚕胚胎早期比较转录组分析. 基因组学与应用生物学. 2021(01): 60-71 .

Other cited types(2)

Get Citation

PDF

XML

Article views PDF downloads Cited by(8)

Turn off MathJax

Article Contents

Abstract

References

Preparation of enrofloxacin nanoemulsion and evaluation of pharmacodynamic of spray administration