- Chinese Core Journal
- Chinese Science Citation Database (CSCD) Source journal
- Journal of Citation Report of Chinese S&T Journals (Core Edition)
| Citation: |
XU Ying, QIU Yangyang, SHEN Yue, et al. Preparation of enrofloxacin nanoemulsion and evaluation of pharmacodynamic of spray administration[J]. Journal of South China Agricultural University, 2021, 42(1): 42-48. DOI: 10.7671/j.issn.1001-411X.202001030
|
To develop a new formulation of enrofloxacin nanoemulsion for spray administration, and evaluate its disease control effect using chicken model of systemic infection.
The phase transition method was used, and single factor and orthogonal tests were performed to screen formulations and optimize the prescription. High-performance liquid chromatography (HPLC) was used to determine enrofloxacin content in nanoemulsion. Nanoparticle and zeta potential analyzer was used to measure the particle size and zeta potential of nanoemulsion. Pharmacodynamic experiments were performed using chicken models infected by Salmonella pullorum and Escherichia coli, and the control effects of spray adminstration of compound enrofloxacin nanoemulsion on bacterial diseases were evaluated.
The nanoemulsion prepared in this experiment was an oil-in-water type, light yellow, clear and transparent, and had uniform droplet distribution. The average particle size was (10.79 ± 1.5) nm, the polydispersity index (PDI) was 0.446 and the zeta potential was −21.63 mV. The pharmacodynamic evaluation showed that the survival rates of preventive administration groups with high, medium and low doses were 90.0%, 83.3% and 73.3% respectively, which were significantly higher than that of the infection control group (26.7%) ( P<0.01).
The compound enrofloxacin nanoemulsion prepared in this study has stable properties. The ultrasonic spray administration has good control effects on chicken diseases due to systemic infection of Salmonella pullorum and Escherichia coli.
| [1] |
SAIF Y M, 主编. 禽病学: 12版[M]. 苏敬良, 高福, 索勋, 译. 北京: 中国农业出版社, 2012: 811-870.
|
| [2] |
崔福德, 主编. 药剂学: 6版[M]. 北京: 人民卫生出版社, 2010: 201-213.
|
| [3] |
CALDERON-NIEVA D, GOONEWARDENE K B, GOMIS S, et al. Veterinary vaccine nanotechnology: Pulmonary and nasal delivery in livestock animals[J]. Drug Deliv Transl Re, 2017, 7(4): 558-570. doi: 10.1007/s13346-017-0400-9
|
| [4] |
VARGA M. Therapeutics[M]//Textbook of Rabbit Medicine: 2nd ed. Oxford: Butterworth-Heinemann, 2014: 137-177.
|
| [5] |
PERRY S M, MITCHELL M A. Antibiotic therapy[M]//Mader's reptile and amphibian medicine and surgery: 2nd Edition. Philadelphia: Saunders, 2019: 1139-1154.
|
| [6] |
陈杖榴. 兽医药理学: 3版[M]. 北京: 中国农业出版社, 2004.
|
| [7] |
MARTINEZ M, MCDERMOTT P, WALKER R. Pharmacology of the fluoroquinolones: A perspective for the use in domestic animals[J]. The Veterinary Journal, 2006, 172(1): 10-28. doi: 10.1016/j.tvjl.2005.07.010
|
| [8] |
方炳虎, 曾振灵, 冯淇辉, 等. 恩诺沙星对鸡大肠杆菌病及葡萄球菌病的药效研究[J]. 中国兽医学报, 1997(2): 54-57.
|
| [9] |
GAUTIER-BOUCHARDON A V, REINHARDT A K, KOBISCH M, et al. In vitro development of resistance to enrofloxacin, erythromycin, tylosin, tiamulin and oxytetracycline in Mycoplasma gallisepticum, Mycoplasma iowae and Mycoplasma synoviae[J]. Veterinary Microbiology, 2002, 88(1): 47-58. doi: 10.1016/S0378-1135(02)00087-1
|
| [10] |
SCHRODER J. Enrofloxacin: A new antimicrobial agent[J]. Journal of the South African Veterinary Association, 1989, 60(2): 122-124.
|
| [11] |
FLECKENSTEIN E, UPHOFF C C, DREXLER H G. Effective treatment of Mycoplasma contamination in cell lines with enrofloxacin (Baytril)[J]. Leukemia, 1994, 8(8): 1424-1434.
|
| [12] |
BAUDITZ R. Results of clinical studies with Bytril in poultry[J]. Veterinary Medical Review, 1987, 2: 130-136.
|
| [13] |
SUMANO L H, OCAMPO C L, BRUMBAUGH G W, et al. Effectiveness of two fluoroquinolones for the treatment of chronic respiratory disease outbreak in broilers[J]. British Poultry Science, 1998, 39(1): 42-46. doi: 10.1080/00071669889376
|
| [14] |
王波臻, 欧阳五庆, 纪俊明, 等. 复方恩诺沙星纳米乳的制备及体外透皮性能考察[J]. 畜牧与兽医, 2016, 48(3): 77-83.
|
| [15] |
毛庆祥, 常文军, 蔡全才. 桉叶油透皮吸收促进剂研究进展[J]. 药学实践杂志, 2003(4): 205-209. doi: 10.3969/j.issn.1006-0111.2003.04.005
|
| [16] |
DHAKAD A K, PANDEY V V, BEG S, et al. Biological, medicinal and toxicological significance of Eucalyptus leaf essential oil: A review[J]. Journal of the Science of Food and Agriculture, 2018, 98(3): 833-848. doi: 10.1002/jsfa.8600
|
| [17] |
朱向波. 鸡白痢沙门氏菌病诊断与防治[J]. 中国畜禽种业, 2015, 11(5): 149-150. doi: 10.3969/j.issn.1673-4556.2015.05.112
|
| [18] |
郑志新. 张家口地区鸡白痢沙门氏菌的分离鉴定及药敏试验[J]. 河北北方学院学报(自然科学版), 2015, 31(3): 36-38.
|
| [19] |
安辉, 张大华, 陈小玲, 等. 鸡白痢沙门氏菌的分离与鉴定[J]. 安徽农业科学, 2011, 39(32): 19888-19890. doi: 10.3969/j.issn.0517-6611.2011.32.091
|
| [20] |
刘吉山, 沈志强, 王燕, 等. 我国部分地区禽致病性大肠杆菌的分离鉴定与药敏试验[J]. 中国家禽, 2005(7): 14-16. doi: 10.3969/j.issn.1004-6364.2005.07.004
|
| [21] |
路卫星. 鸡大肠杆菌病的诊断与治疗[J]. 中国兽医杂志, 2015, 51(8): 43-44. doi: 10.3969/j.issn.0529-6005.2015.08.015
|
| [22] |
陈洪科. 禽病诊断和防治: 1版[M]. 北京: 中国农业科技出版社, 1996.
|
| [23] |
刘慕欣, 付领新. 喷雾给药治疗禽类呼吸道疾病的效果观察[J]. 当代畜牧, 2014(8): 76-77.
|
| [24] |
DAS A, AHMED A B. Natural permeation enhancer for transdermal drug delivery system and permeation evaluation: A review[J]. Asian Journal of Pharmaceutical and Clinical Research, 2017, 10(9): 5. doi: 10.22159/ajpcr.2017.v10i9.19389
|
| [25] |
陈宇. 桉叶挥发油制剂开发及其抗慢性支气管炎的药效研究[D]. 天津: 天津大学, 2008.
|
| [26] |
SADLON A E, LAMSON D W. Immune-modifying and antimicrobial effects of Eucalyptus oil and simple inhalation devices[J]. Alternative Medicine Review: A Journal of Clinical Therapeutic, 2010, 15(1): 33.
|
| [27] |
欧阳五庆, 杨增岐, 李雅. 恩诺沙星对人工诱发的鸡大肠杆菌病的疗效[J]. 西北农业大学学报, 1998: 76-79.
|
Supported by: Beijing Renhe Information Technology Co., Ltd. 
DownLoad: