Citation: | GUO Conghui, LI Qinghua, DING Linshu, et al. Toxic effects of perfluorooctanoic acid on mouse oocytes[J]. Journal of South China Agricultural University, 2022, 43(1): 53-58. DOI: 10.7671/j.issn.1001-411X.202101028 |
The purpose of this study was to investigate the toxic effect of perfuorooctanoic aid(PFOA) on mouse oocytes.
One hundred and sixty 6-week-old Kunming female mice with similar body weight were divided randomly into four groups, with five replicates in each group and eight mice in each replicate. After 7 days of adaptation, the mice were fed with different doses of PFOA for 14 days. The daily doses for control, low, medium, and high dose groups were 0, 5, 10 and 20 mg/kg respectively. Then the mice were superovulated with injection of pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG), and the oocytes were obtained for detection.
Compared to the control group, the oocyte maturation rates of mice in the middle and high dose groups decreased by 14.28% and 28.17% respectively, while the contents of reactive oxygen species in oocytes increased by 135% and 177% respectively. The proportions of β-tubulin with abnormal morphology and irregular chromosomes in oocytes increased by 65.06% and 75.60% respectively. In addition, the proportions of (Phospho-histone H2A.X, P-H2A.X) in oocytes of low, medium and high dose groups increased by 47%, 133% and 1.71% respectively compared with the control.
Mice fed with PFOA may decrease their oocyte maturation rate by inducing the increase of reactive oxygen species, DNA double strand break and cytoskeleton damage in oocytes.
[1] |
GIESY J P, KANNAN K, JONES P D. Global biomonitoring of perfluorinated organics[J]. Scientific WorldJournal, 2001, 1: 627-629.
|
[2] |
KOSKELA A, FINNILA M A, KORKALAINEN M, et al. Effects of developmental exposure to perfluorooctanoic acid (PFOA) on long bone morphology and bone cell differentiation[J]. Toxicology and Applied Pharmacology, 2016, 301: 14-21. doi: 10.1016/j.taap.2016.04.002
|
[3] |
FROMME H, TITTLEMIER S A, VOLKEL W, et al. Perfluorinated compounds: Exposure assessment for the general population in Western countries[J]. International Journal of Hygiene & Environmental Health, 2009, 212(3): 239-270.
|
[4] |
夏慧, 敖俊杰, 袁涛. 室内灰尘中全氟化合物的污染状况与人体暴露水平评估[J]. 生态毒理学报, 2016, 11(2): 223-230.
|
[5] |
杜国勇, 蒋小萍, 卓丽, 等. 长江流域重庆段水体中全氟化合物的污染特征及风险评价[J]. 生态环境学报, 2019, 28(11): 2266-2272.
|
[6] |
陈诗艳, 仇雁翎, 朱志良, 等. 土壤中全氟和多氟烷基化合物的污染现状及环境行为[J]. 环境科学研究, 2021, 34(2): 468-478.
|
[7] |
JOGSTEN I E, PERELLO G, LLEBARIA X, et al. Exposure to perfluorinated compounds in Catalonia, Spain, through consumption of various raw and cooked foodstuffs, including packaged food[J]. Food and chemical toxicology, 2009, 47(7): 1577-1583. doi: 10.1016/j.fct.2009.04.004
|
[8] |
BACH C C, VESTED A, JORGENSEN K T, et al. Perfluoroalkyl and polyfluoroalkyl substances and measures of human fertility: A systematic review[J]. CRC Critical Reviews in Toxicology, 2016, 46(9): 735-755. doi: 10.1080/10408444.2016.1182117
|
[9] |
ZHANG S, TAN R, PAN R, et al. Association of perfluoroalkyl and polyfluoroalkyl substances with premature ovarian insufficiency in Chinese women[J]. The Journal of Clinical Endocrinology and Metabolism, 2018, 103(7): 2543-2551. doi: 10.1210/jc.2017-02783
|
[10] |
CHEN Y, ZHOU L, XU J, et al. Maternal exposure to perfluorooctanoic acid inhibits luteal function via oxidative stress and apoptosis in pregnant mice[J]. Reproductive Toxicology, 2017, 69: 159-166. doi: 10.1016/j.reprotox.2017.02.010
|
[11] |
DU G, HU J, HUANG Z, et al. Neonatal and juvenile exposure to perfluorooctanoate (PFOA) and perfluorooctane sulfonate (PFOS): Advance puberty onset and kisspeptin system disturbance in female rats[J]. Ecotoxicology and Environmental Safety, 2019, 167: 412-421. doi: 10.1016/j.ecoenv.2018.10.025
|
[12] |
KANG Q, GAO F, ZHANG X, et al. Nontargeted identification of per- and polyfluoroalkyl substances in human follicular fluid and their blood-follicle transfer[J]. Environment International, 2020, 139: 105686. doi: 10.1016/j.envint.2020.105686
|
[13] |
RASHID F, RAMAKRISHNAN A, FIELDS C, et al. Acute PFOA exposure promotes epigenomic alterations in mouse kidney tissues[J]. Toxicology Reports, 2020, 7: 125-132. doi: 10.1016/j.toxrep.2019.12.010
|
[14] |
XU W, LI L, SUN J, et al. Putrescine delays postovulatory aging of mouse oocytes by upregulating PDK4 expression and improving mitochondrial activity[J]. Aging (Albany NY), 2018, 10(12): 4093-4106.
|
[15] |
LOPEZ-ARELLANO P, LOPEZ-ARELLANO K, LUNA J, et al. Perfluorooctanoic acid disrupts gap junction intercellular communication and induces reactive oxygen species formation and apoptosis in mouse ovaries[J]. Environmental Toxicology, 2019, 34(1): 92-98. doi: 10.1002/tox.22661
|
[16] |
YANG L L, ZHAO Y, LUO S M, et al. Toxic effects and possible mechanisms of hydrogen sulfide and/or ammonia on porcine oocyte maturation in vitro[J]. Toxicology Letters, 2018, 285: 20-26. doi: 10.1016/j.toxlet.2017.12.019
|
[17] |
YU Y, CUI Y, NIEDERNHOFER L J, et al. Occurrence, biological consequences, and human health relevance of oxidative stress-induced DNA damage[J]. Chemical Research in Toxicology, 2016, 29(12): 2008-2039. doi: 10.1021/acs.chemrestox.6b00265
|
[18] |
DING Z M, ZHANG S X, JIAO X F, et al. Doxorubicin exposure affects oocyte meiotic maturation through DNA damage induced meiotic arrest[J]. Toxicological Sciences, 2019, 171(2): 359-368.
|
[19] |
DING Z M, JIAO X F, WU D, et al. Bisphenol AF negatively affects oocyte maturation of mouse in vitro through increasing oxidative stress and DNA damage[J]. Chemico-Biological Interactions, 2017, 278: 222-229. doi: 10.1016/j.cbi.2017.10.030
|
[20] |
JEONG P S, LEE S, PARK S H, et al. Butylparaben is toxic to porcine oocyte maturation and subsequent embryonic development following in vitro fertilization[J]. International Journal of Molecular Sciences, 2020, 21(10): 3692. doi: 10.3390/ijms21103692
|
[21] |
JIAO X, DING Z, MENG F, et al. The toxic effects of Fluorene-9-bisphenol on porcine oocyte in vitro maturation[J]. Environmental Toxicology, 2020, 35(2): 152-158. doi: 10.1002/tox.22851
|
[22] |
TRANFO G, CAPOROSSI L, PACI E, et al. Urinary phthalate monoesters concentration in couples with infertility problems[J]. Toxicology Letters, 2012, 213(1): 15-20. doi: 10.1016/j.toxlet.2011.11.033
|
[23] |
BENNABI I, TERRET M E, VERLHAC M H. Meiotic spindle assembly and chromosome segregation in oocytes[J]. Journal of Cell Biology, 2016, 215(5): 611-619. doi: 10.1083/jcb.201607062
|
[24] |
AZOURY J, VERLHAC M H, DUMONT J. Actin filaments: Key players in the control of asymmetric divisions in mouse oocytes[J]. Biology of the Cell, 2009, 101(2): 69-76. doi: 10.1042/BC20080003
|
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