| Citation: |
PENG Ziai, LI Dandan, XIA Aoyun, et al. Study on magnetic nanoparticle loading plasmid DNA[J]. Journal of South China Agricultural University, 2020, 41(1): 78-82. DOI: 10.7671/j.issn.1001-411X.20190518
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To study biophysical property, loading morphology and loading mechanism of nanocarrier-gene complex prepared by magnetic nanoparticle (MNP) loading plasmind DNA, and lay a foundation for application of oriented editing technology based on magnetic nano carrier.
MNP was used as gene carrier to prepare nanocarrier-gene complex MNP-pRGEB32 with plasmid DNA pRGEB32. The abilities of MNP loading and protecting pRGEB32 were analyzed. The particle size distribution, Zeta potential and loading morphology of MNP-pRGEB32 were investigated.
MNP compressed, adsorbed and aggregated pRGEB32 through electrostatic interaction to form nanocarrier-gene complex. MNP could effectively load and protect pRGEB32.
MNP can be used as an ideal gene transfer carrier.
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