| Citation: |
YUE Dong, XIE Peipei, LU Jingyi, et al. Effects of α-ketoglutaric acid produced by solid fermentation on mouse growth, intestinal structure and immune function[J]. Journal of South China Agricultural University, 2020, 41(2): 1-9. DOI: 10.7671/j.issn.1001-411X.201905020
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To screen strains for producing α-ketoglutaric acid by solid fermentation, and study the effects of fermentation products on animal growth, intestinal structure and immune function.
Nine strains (including lactic acid bacteria, yeast and Aspergillus) were fermented and α-ketoglutaric acid content in the fermentation products was detected. Succinic acid was added during the fermentation to study its effect on the production rate of α-ketoglutaric acid. Subsequently, sixteen 4-week-old C57/BL mice were randomly divided into two groups, 5% normal fermented material or 5% α-ketoglutarate-rich fermented material was added to the common diet for four weeks. Animal feeding and growth, intestinal structure, and phagocytosis and blood picture of monocyte-macrophages were analyzed.
The solid fermentation of yeast produced a trace amount of α-ketoglutaric acid, Bacillus subtilis produced about 60 μg/g α-ketoglutaric acid, and α-ketoglutaric acid was not detected using other strains. The addition of succinic acid to the solid fermentation of Bacillus subtilis promoted the accumulation of α-ketoglutaric acid with the maximum production rate of 0.3%. Dietary supplementation of 5% targeted α-ketoglutarate fermentation product slightly reduced body weight and body weight gain of mice(P>0.05), significantly increased the soleus muscle weight of mice (P<0.05), and significantly reduced brown fat weight in mice (P<0.05). The fermentation product had no significant effect on the villus length and crypt depth of mouse ileum and jejunum. Blood and immunological indicators revealed thatα-ketoglutarate-rich fermentation product significantly reduced the percentage of neutrophils, increased the percentage of lymphocyte, and increased the content of red blood cell hemoglobin. Moreover, the fermentation product also significantly increased the phagocytic function of mouse monocyte-macrophages (P<0.05).
The addition of succinic acid can promote accumulation of α-ketoglutaric acid in the solid fermentation process of Bacillus subtilis. Adding α-ketoglutarate-rich fermentation in the diet can significantly increase the muscle weight, decrease the fat weight of mice, and significantly improve the immune function of mice.
| [1] |
YEH S L, LAI Y N, SHANG H F, et al. Effects of glutamine supplementation on innate immune response in rats with gut-derived sepsis[J]. Brit J Nutr, 2004, 91(3): 423-429. doi: 10.1079/BJN20031069
|
| [2] |
陈家顺, 苏文璇, 符晨星, 等. α−酮戊二酸的生理功能及其在动物生产中的应用[J]. 动物营养学报, 2018, 30(10): 29-38.
|
| [3] |
CHEN J, SU W, KANG B, et al. Supplementation with alpha-ketoglutarate to a low-protein diet enhances amino acid synthesis in tissues and improves protein metabolism in the skeletal muscle of growing pigs[J]. Amino acids, 2018, 50(11): 1525-1537. doi: 10.1007/s00726-018-2618-3
|
| [4] |
SPENCER G J, MCGRATH C J, GENEVER P G. Current perspectives on NMDA-type glutamate signalling in bone[J]. Int J Biochem Cell Biol, 2007, 39(6): 1089-1104. doi: 10.1016/j.biocel.2006.11.002
|
| [5] |
CHIN R M, FU X, PAI M Y, et al. The metabolite alpha-ketoglutarate extends lifespan by inhibiting ATP synthase and TOR[J]. Nature, 2014, 510(7505): 397-401. doi: 10.1038/nature13264
|
| [6] |
RAIMUNDO N, BAYSAL B E, SHADEL G S. Revisiting the TCA cycle: Signaling to tumor formation[J]. Trends Mol Med, 2011, 17(11): 641-649. doi: 10.1016/j.molmed.2011.06.001
|
| [7] |
SAUER M, PORRO D, MATTANOVICH D, et al. Microbial production of organic acids: Expanding the markets[J]. Trends Biotechnol, 2008, 26(2): 100-108. doi: 10.1016/j.tibtech.2007.11.006
|
| [8] |
郭洪伟, 堵国成, 周景文, 等. 微生物发酵生产α−酮戊二酸研究进展[J]. 生物工程学报, 2013, 29(2): 141-152.
|
| [9] |
刘艳新, 刘占英, 倪慧娟, 等. 微生物发酵饲料的研究进展与前景展望[J]. 饲料博览, 2017(2): 15-22. doi: 10.3969/j.issn.1001-0084.2017.02.005
|
| [10] |
MORGUNOV I G, KAMZOLOVA S V, PEREVOZNIKOVA O A, et al. Pyruvic acid production by a thiamine auxotroph of Yarrowia lipolytica[J]. Process Biochem, 2004, 39(11): 1469-1474. doi: 10.1016/S0032-9592(03)00259-0
|
| [11] |
CHERNYAVSKAYA O G, SHISHKANOVA N V, IL'CHENKO A P, et al. Synthesis of alpha-ketoglutaric acid by Yarrowia lipolytica yeast grown on ethanol[J]. Appl Microbiol Biotechnol, 2000, 53(2): 152-158. doi: 10.1007/s002530050002
|
| [12] |
LOCKWOOD L B, STODOLA F H. Preliminary studies on the production of alpha-ketoglutaric acid by Pseudomonas fluorescens[J]. J Biol Chem, 1946, 164(1): 81-83.
|
| [13] |
LIU Y, HUANG J, HOU Y, et al. Dietary arginine supplementation alleviates intestinal mucosal disruption induced by Escherichia coli lipopolysaccharide in weaned pigs[J]. Br J Nutr, 2008, 100(3): 552-560. doi: 10.1017/S0007114508911612
|
| [14] |
BERGEN W G, WU G. Intestinal nitrogen recycling and utilization in health and disease[J]. J Nutr, 2009, 139(5): 821-825. doi: 10.3945/jn.109.104497
|
| [15] |
FLYNN N E, BIRD J G, GUTHRIE A S. Glucocorticoid regulation of amino acid and polyamine metabolism in the small intestine[J]. Amino acids, 2009, 37(1): 123-129. doi: 10.1007/s00726-008-0206-7
|
| [16] |
KRISTENSEN N B, JUNGVID H, FERNANDEZ J A, et al. Absorption and metabolism of alpha-ketoglutarate in growing pigs[J]. J Anim Physiol Ani N, 2002, 86(7/8): 239-245.
|
| [17] |
HOU Y, YAO K, WANG L, et al. Effects of alpha-ketoglutarate on energy status in the intestinal mucosa of weaned piglets chronically challenged with lipopolysaccharide[J]. Br J Nutr, 2011, 106(3): 357-363. doi: 10.1017/S0007114511000249
|
| [18] |
HOU Y, WANG L, DING B, et al. Dietary alpha-ketoglutarate supplementation ameliorates intestinal injury in lipopolysaccharide-challenged piglets[J]. Amino acids, 2010, 39(2): 555-564. doi: 10.1007/s00726-010-0473-y
|
| [19] |
PARRY-BILLINGS M, EVANS J, CALDER P C, et al. Does glutamine contribute to immunosuppression after major burns[J]. Lancet, 1990, 336(8714): 523-525. doi: 10.1016/0140-6736(90)92083-T
|
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