Antifungal activity of <i>Mikania micrantha</i> extract against <i>Magnaporthe oxyzae</i>

YANG Taijiao; ZHANG Wei; LUO Jianmei; LI Qing; NIE Shanshan; LIAO Shimei; FU Tao; YAN Xiaohui

doi:10.7671/j.issn.1001-411X.202311015

Journal of South China Agricultural University > 2025 > 46(1): 81-88. > DOI: 10.7671/j.issn.1001-411X.202311015

YANG Taijiao, ZHANG Wei, LUO Jianmei, et al. Antifungal activity of Mikania micrantha extract against Magnaporthe oxyzae[J]. Journal of South China Agricultural University, 2025, 46(1): 81-88. DOI: 10.7671/j.issn.1001-411X.202311015

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Antifungal activity of Mikania micrantha extract against Magnaporthe oxyzae

Key Laboratory of Forest Disaster Warning and Control in Universities of Yunnan Province/College of Forestry, Southwest Forestry University, Kunming 650224, China

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Received Date: November 14, 2023
Available Online: December 10, 2024
Published Date: December 12, 2024

Graphical Abstract

Abstract

Abstract

Objective
Mikania micrantha, an invasive species, was used as a material to investigate the antifungal activity of different extracts and components against Magnaporthe oxyzae.

Method
The antifungal activity of M. micrantha extract from Dehong of Yunnan Province was determined by growth rate method with M. oxyzae as the test pathogen, and the antifungal active components of the extract were traced by column chromatography.
Result
At the initial screening mass concentration of 1 mg/mL, M. micrantha ethyl acetate extract showed potent antifungal activity against M. oxyzae, with the inhibition ratio of 49.84%. The antifungal activity tracking of 14 column chromatography components of the ethyl acetate extract showed that the antifungal activities of Fr₅, Fr₆, Fr₁₂ and Fr₁₃ were significant, EC₅₀ values on day 9 after inoculation were 1.691, 2.134, 0.865 and 0.818 mg/mL, respectively. All the four active components resulted in the reduction of mycelial mass, elevation of MDA content, aberration of mycelial morphology.
Conclusion
This study finds that M. micrantha can significantly inhibit M. oxyzae, which provides a new idea for comprehensive development and utilization of M. micrantha, and also provides a scientific basis for green control of M. oxyzae.
- Plant extract,
- Magnaporthe oxyzae,
- Mikania micrantha,
- Growth rate method,
- Antifungal activity

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References

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