Antifungal activity of Mikania micrantha extract against Magnaporthe oxyzae

Objective Mikania micrantha, an invasive species, was used as a material to investigate the antifungal activity of different extracts and components against Magnaporthe oxyzae.

Method The antifungal activity of M. micrantha extract from Dehong of Yunnan Province was determined by growth rate method with M. oxyzae as the test pathogen, and the antifungal active components of the extract were traced by column chromatography.

Result At the initial screening mass concentration of 1 mg/mL, M. micrantha ethyl acetate extract showed potent antifungal activity against M. oxyzae, with the inhibition ratio of 49.84%. The antifungal activity tracking of 14 column chromatography components of the ethyl acetate extract showed that the antifungal activities of Fr₅, Fr₆, Fr₁₂ and Fr₁₃ were significant, EC₅₀ values on day 9 after inoculation were 1.691, 2.134, 0.865 and 0.818 mg/mL, respectively. All the four active components resulted in the reduction of mycelial mass, elevation of MDA content, aberration of mycelial morphology.

Conclusion This study finds that M. micrantha can significantly inhibit M. oxyzae, which provides a new idea for comprehensive development and utilization of M. micrantha, and also provides a scientific basis for green control of M. oxyzae.