Disease resistance of SlJAZ7 and its interaction with SlTGA7 in tomato

LIN Chenyu; GUO Xin; WANG Wenjuan; ZHAI Min; CAO Yingjie; WANG Mengyue; ZHAO Pingjuan; YU Xiaohui

doi:10.7671/j.issn.1001-411X.202312017

Journal of South China Agricultural University > 2024 > 45(4): 525-534. > DOI: 10.7671/j.issn.1001-411X.202312017

LIN Chenyu, GUO Xin, WANG Wenjuan, et al. Disease resistance of SlJAZ7 and its interaction with SlTGA7 in tomato[J]. Journal of South China Agricultural University, 2024, 45(4): 525-534. DOI: 10.7671/j.issn.1001-411X.202312017

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Disease resistance of SlJAZ7 and its interaction with SlTGA7 in tomato

LIN Chenyu^{1, 2,},
GUO Xin¹,
WANG Wenjuan^{1, 2},
ZHAI Min²,
CAO Yingjie^{1, 2},
WANG Mengyue^{1, 2},
ZHAO Pingjuan^2, ,,
YU Xiaohui^1, ,

1.
College of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
2.
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences/National Key Laboratory for Tropical Crop Breeding, Haikou 571101, China

More Information

Received Date: December 13, 2023
Available Online: March 26, 2024
Published Date: April 07, 2024

Abstract

Abstract

Objective
Bacterial spot disease is one of the main factors leading to tomato (Solanum lycopersicum) yield reduction, and Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) is one of the pathogenic factors of bacterial spot disease. Transient silencing of JAZ7 gene in tomato results in its increased susceptibility to Pst DC3000. At present, there are a few reports to verify the resistance function and mechanism of JAZ7 gene in tomato. In this study, we cloned SlJAZ7 gene in tomato leaves, and a stable genetic transgenic tomato with overexpression of SlJAZ7 was created to analyze its resistance function and mechanism, and the relationship between SlJAZ7 and SlTGA7, which is closely related to disease resistance, was studied at the transcriptional and protein levels, providing a theoretical basis for effective prevention and control of bacterial spot disease.
Method
The resistance function of SlJAZ7 gene was determined by the phenotypic difference between wild type and transgenic tomato inoculated with Pst DC3000. RT-qPCR was used to analyze the tissue-specific expression of SlJAZ7 gene and SlTGA7 gene under Pst DC3000, methyl jasmonate (MeJA) and salicylic acid (SA) treatments. The subcellular localization of SIJAZ7 and SlTGA7 proteins was studied by transient expression of tobacco. The interaction between SlJAZ7 and SlTGA7 was verified by Y2H, BiFC and pull down experiments, to verify the disease resistance function and possible mechanism of SlJAZ7.
Result
Transgenic tomato leaves overexpressing SlJAZ7 gene had lower peroxidation damage than wild type when treated with pathogens, the expression level of SlTGA7 in transgenic lines increased. SlJAZ7 gene was highly expressed in nutrient tissues. SlJAZ7 gene was induced by Pst DC3000 and in response to MeJA and SA treatments. SlTGA7 gene showed opposite change trend under the same treatment. Both SlJAZ7 and SlTGA7 proteins were located in the nucleus. All Y2H, BiFC and pull down experiments proved the interaction between SlJAZ7 and SlTGA7.
Conclusion
Overexpression of SlJAZ7 gene can reduce the accumulation of reactive oxygen species and improve tomato disease resistance. SlJAZ7 positively regulates SlTGA7 gene expression at the transcriptional level. SlJAZ7 interacts with SlTGA7. It is speculated that SIJAZ7 gene may improve the disease resistance of transgenic tomato by increasing the expression level of SITGA7 gene during pathogen infection, initiating the expression of downstream resistance genes of SITGA7, or by binding with SITGA7 protein, affecting its regulation of MYC and other transcription factors. The result lays a foundation for further research on the mechanism of SlJAZ7.
- Tomato,
- SlJAZ7,
- Protein interaction,
- Disease resistance,
- Methyl jasmonate,
- Salicylic acid

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References (36)

References

[1]	LIN T, ZHU G T, ZHANG J H, et al. Genomic analyses provide insights into the history of tomato breeding[J]. Nature Genetics, 2014, 46(11): 1220-1226. doi: 10.1038/ng.3117
[2]	FRIEDMAN M. Tomato glycoalkaloids: Role in the plant and in the diet[J]. Journal of Agricultural and Food Chemistry, 2002, 50(21): 5751-5780. doi: 10.1021/jf020560c
[3]	WAI K P P, SIDDIQUE M I, MO H S, et al. Pathotypes of bacterial spot pathogen infecting capsicum peppers in Korea[J]. Plant Pathology Journal, 2015, 31(4): 428-432. doi: 10.5423/PPJ.NT.05.2015.0074
[4]	XIN X F, HE S Y. Pseudomonas syringae pv. tomato DC3000: A model pathogen for probing disease susceptibility and hormone signaling in plants[J]. Annual Review of Phytopathology, 2013, 51: 473-498. doi: 10.1146/annurev-phyto-082712-102321
[5]	BEALE M H, WARD J L. Jasmonates: Key players in the plant defence[J]. Natural Product Reports, 1998, 15(6): 533-548. doi: 10.1039/a815533y
[6]	CREELMAN R A, MULLET J E. Oligosaccharins, brassinolides, and jasmonates: Nontraditional regulators of plant growth, development, and gene expression[J]. Plant Cell, 1997, 9(7): 1211-1223. doi: 10.1105/tpc.9.7.1211
[7]	BROWSE J. Jasmonate passes muster: A receptor and targets for the defense hormone[J]. Annual Review of Plant Biology, 2009, 60: 183-205. doi: 10.1146/annurev.arplant.043008.092007
[8]	ZHANG F, YAO J, KE J Y, et al. Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling[J]. Nature, 2015, 525(7568): 269-273. doi: 10.1038/nature14661
[9]	SUN Y G, LIU C X, LIU Z B, et al. Characterization and expression analysis of the JAZ gene family in resistance to gray leaf spots in tomato[J]. International Journal of Molecular Sciences, 2021, 22(18): 9974. doi: 10.3390/ijms22189974
[10]	裴童. JAZ3在番茄抗叶霉病免疫应答过程中的功能及调控机制研究[D]. 哈尔滨: 东北农业大学, 2021.
[11]	THATCHER L F, CEVIK V, GRANT M, et al. Characterization of a JAZ7 activation-tagged Arabidopsis mutant with increased susceptibility to the fungal pathogen Fusarium oxysporum[J]. Journal of Experimental Botany, 2016, 67(8): 2367-2386. doi: 10.1093/jxb/erw040
[12]	ISHIGA Y, ISHIGA T, UPPALAPATI S R, et al. Jasmonate ZIM-domain (JAZ) protein regulates host and nonhost pathogen-induced cell death in tomato and Nicotiana benthamiana[J]. PLoS One, 2013, 8(9): e75728. doi: 10.1371/journal.pone.0075728
[13]	ALLU A D, BROTMAN Y, XUE G P, et al. Transcription factor ANAC032 modulates JA/SA signalling in response to Pseudomonas syringae infection[J]. EMBO Reports, 2016, 17(11): 1578-1589. doi: 10.15252/embr.201642197
[14]	ZHANG N L, ZHOU S, YANG D Y, et al. Revealing shared and distinct genes responding to JA and SA signaling in Arabidopsis by Meta-Analysis[J]. Frontiers in Plant Science, 2011, 11: 908.
[15]	田义, 张彩霞, 康国栋, 等. 植物TGA转录因子研究进展[J]. 中国农业科学, 2016, 49(4): 632-642.
[16]	KATAGIRI F, LAM E, CHUA N H. Two tobacco DNA-binding proteins with homology to the nuclear factor CREB[J]. Nature, 1989, 340(6236): 727-730. doi: 10.1038/340727a0
[17]	SHEARER H L, WANG L P, DELONG C, et al. NPR1 enhances the DNA binding activity of the Arabidopsis bZIP transcription factor TGA7[J]. Botany, 2009, 87(6): 561-570. doi: 10.1139/B08-143
[18]	ZHOU J M, TRIFA Y, SILVA H, et al. NPR1 differentially interacts with members of the TGA/OBF family of transcription factors that bind an element of the PR-1 gene required for induction by salicylic acid[J]. Molecular Plant-Microbe Interactions, 2000, 13(2): 191-202. doi: 10.1094/MPMI.2000.13.2.191
[19]	JOHNSON C, BODEN E, ARIAS J. Salicylic acid and NPR1 induce the recruitment of trans-activating TGA factors to a defense gene promoter in Arabidopsis[J]. Plant Cell, 2003, 15(8): 1846-1858. doi: 10.1105/tpc.012211
[20]	ZANDER M, LA CAMERA S, LAMOTTE O, et al. Arabidopsis thaliana class-II TGA transcription factors are essential activators of jasmonic acid/ethylene-induced defense responses[J]. Plant Journal, 2009, 61(2): 200-210. doi: 10.1111/j.1365-313X.2009.04044.x
[21]	JIANG H, GU S Y, LI K, et al. Two TGA transcription factor members from hyper-susceptible soybean exhibiting significant basal resistance to soybean mosaic virus[J]. International Journal of Molecular Sciences, 2021, 22(21): 11329. doi: 10.3390/ijms222111329
[22]	LIU W, ZHAO C, LIU L, et al. Genome-wide identification of the TGA gene family in kiwifruit (Actinidia chinensis spp. ) and revealing its roles in response to Pseudomonas syringae pv. actinidiae (Psa) infection[J]. International Journal of Biological Macromolecules, 2022, 222: 101-113. doi: 10.1016/j.ijbiomac.2022.09.154
[23]	OLSON B J S C. Assays for determination of protein concentration[J]. Current Protocols in Pharmacology, 2016, 73. doi: 10.1002/cpph.3.
[24]	ZHANG Y, FAN W, KINKEMA M, et al. Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene[J]. Proceedings of the National Academy of Sciences of the United States of America, 1996, 96(11): 6523-6528.
[25]	SUN T J, BUSTA L, ZHANG Q, et al. TGACG-BINDING FACTOR 1 (TGA1) and TGA4 regulate salicylic acid and pipecolic acid biosynthesis by modulating the expression of SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1 (SARD1) and CALMODULIN-BINDING PROTEIN 60g (CBP60g)[J]. New Phytologist, 2017, 217(1): 344-354.
[26]	段军叶. 转录因子TGA7参与拟南芥响应干旱胁迫的分子机制研究[D]. 北京: 中国农业大学, 2016.
[27]	吴娟娟. 拟南芥转录因子TGA7参与植物响应干旱胁迫的机制研究[D]. 北京: 中国农业大学, 2014.
[28]	侯佳音. 番茄TGA2转录因子在农药百菌清代谢降解中的功能及调控机制[D]. 杭州: 浙江大学, 2019.
[29]	SHEARER H L, CHENG Y T, WANG L P, et al. Arabidopsis clade I TGA transcription factors regulate plant defenses in an NPR1-independent fashion[J]. Molecular Plant-Microbe Interactions, 2012, 25(11): 1459-1468. doi: 10.1094/MPMI-09-11-0256
[30]	高苗苗, 郭鑫, 朱寿松, 等. 番茄SlJAZ11的表达分析及互作蛋白的筛选与验证[J]. 核农学报, 2023, 37(3): 483-494.
[31]	KOORNNEEF A, LEON-REYES A, RITSEMA T, et al. Pieterse, kinetics of salicylate-mediated suppression of jasmonate signaling reveal a role for redox modulation[J]. Plant Physiology, 2008, 147(3): 1358-1368. doi: 10.1104/pp.108.121392
[32]	NIKI T, MITSUHARA I, SEO S, et al. Antagonistic effect of salicylic acid and jasmonic acid on the expression of pathogenesis-related (PR) protein genes in wounded mature tobacco leaves[J]. Plant Cell Physiology, 1998, 39(5): 500-507. doi: 10.1093/oxfordjournals.pcp.a029397
[33]	VAN WEES S C, DE SWART E A, VAN PELT J A, et al. Enhancement of induced disease resistance by simultaneous activation of salicylate- and jasmonate-dependent defense pathways in Arabidopsis thaliana[J]. Proceedings of the National Academy of Sciences of the United States of America, 2000, 97(15): 8711-8716.
[34]	HU Y, LIU Y, TAO J J, et al. GmJAZ3 interacts with GmRR18a and GmMYC2a to regulate seed traits in soybean[J]. Journal of Integrative Plant Biology, 2023, 65(8): 1983-2000. doi: 10.1111/jipb.13494
[35]	CHINI A, GIMENEZ-IBANEZ S, GOOSSENS A, et al. Redundancy and specificity in jasmonate signalling[J], Current Opinion in Plant Biology, 2016, 33: 147-156.
[36]	YANG J, DUAN G H, LI C Q, et al. The crosstalks between jasmonic acid and other plant hormone signaling highlight the involvement of jasmonic acid as a core component in plant response to biotic and abiotic stresses[J]. Frontiers in Plant Science, 2019, 10: 1349. doi: 10.3389/fpls.2019.01349

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Corresponding author: YU Xiaohui, xiaohuiyu@hainanu.edu.cn

1.
College of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China

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Disease resistance of SlJAZ7 and its interaction with SlTGA7 in tomato

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