<i>Pcgf2</i> promotes the  reprogramming of chicken embryonic  fibroblasts  into induced pluripotent stem cells

YAO Ziqi; LIAO Liqin; SONG Jiabei; ZHANG Xinheng; XIE Qingmei

doi:10.7671/j.issn.1001-411X.202304045

Journal of South China Agricultural University > 2024 > 45(3): 311-320. > DOI: 10.7671/j.issn.1001-411X.202304045

YAO Ziqi, LIAO Liqin, SONG Jiabei, et al. Pcgf2 promotes the reprogramming of chicken embryonic fibroblasts into induced pluripotent stem cells[J]. Journal of South China Agricultural University, 2024, 45(3): 311-320. DOI: 10.7671/j.issn.1001-411X.202304045

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Pcgf2 promotes the reprogramming of chicken embryonic fibroblasts into induced pluripotent stem cells

College of Animal Science, South China Agricultural University/Heyuan Branch, Guangdong Provincial Laboratory of Modern Agricultural Science and Technology of Lingnan/Key Laboratory of Genomics and Molecular Breeding of Agricultural Animals in Guangdong Province, Guangzhou 510642, China

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Received Date: April 29, 2023
Available Online: March 03, 2024
Published Date: March 10, 2024

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Abstract

Abstract

Objective
We aimed to improve the stemness of chicken induced pluripotent stem cells using the Pcgf2 gene together with conventional reprogramming factors to induce chicken pluripotent stem cells.
Method
Six conventional reprogramming factors including Oct4, Sox2, Nanog, Lin28, C-myc and Klf4 as well as Pcgf2 gene were amplified and reconstituted with lentiviral vectors as lentiviral plasmids using chicken early embryos as templates. After packaging the lentivirus and measuring the corresponding viral titers, chicken embryonic fibroblasts were infected in groups and stem cell markers were assayed during the induction process.
Result
The cells induced by the combination of conventional reprogramming factors and Pcgf2 all expressed phosphatase activity early. Inducing the pluripotent stem cells with Pcgf2 could make the cells appear stem-like morphology earlier, and the expressions of endogenous genes Oct4, Sox2, Nanog and Lin28 increased more stably.
Conclusion
Pcgf2 can effectively enhance the reprogramming of cells to approach the stem cell state after being added to the induction system. The research provides a theoretical reference for the selection of reprogramming factors to induce complete reprogramming of cells in chickens and the mechanism study.
- Chicken,
- Lentivirus,
- Pluripotent transcription factor,
- Chicken embryonic fibroblast,
- Somatic cell reprogramming,
- Induced pluripotent stem cell

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Pcgf2 promotes the reprogramming of chicken embryonic fibroblasts into induced pluripotent stem cells

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