Identification of endophytic fungus <i>Chaetomium</i> sp. Eef-10 from <i>Eucalyptus exserta</i> and analysis of its active ingredients

OUYANG Jinkui; WU Chunyin; WANG Yuyang; ZHANG Chengbin; MAO Ziling; SHAN Tijiang

doi:10.7671/j.issn.1001-411X.201905004

Journal of South China Agricultural University > 2020 > 41(2): 104-110. > DOI: 10.7671/j.issn.1001-411X.201905004

OUYANG Jinkui, WU Chunyin, WANG Yuyang, et al. Identification of endophytic fungus Chaetomium sp. Eef-10 from Eucalyptus exserta and analysis of its active ingredients[J]. Journal of South China Agricultural University, 2020, 41(2): 104-110. DOI: 10.7671/j.issn.1001-411X.201905004

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Identification of endophytic fungus Chaetomium sp. Eef-10 from Eucalyptus exserta and analysis of its active ingredients

College of Forestry and Landscape Architecture, South China Agricultural University/Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou 510642, China

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Received Date: May 04, 2019
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To determine the classification status of endophytic fungus Eef-10. To isolate and identify secondary metabolites of Eef-10 and evaluate their antibacterial and antitumor activities in order to obtain natural active compounds.

Method
The endophytic fungus was identified by combining morphology and molecular biology methods. The secondary metabolites were mainly separated and purified by vacuum silica gel column chromatography, sephadex LH-20 column chromatography and semi-preparative HPLC. The compounds were identified mainly based on ¹H NMR and ¹³C NMR spectral data as well as related references. The antibacterial activities against five different test bacteria were determined by the MTT method and the antitumor activities against two cancer cells were determined by the CCK8 method.

Result
Three compounds were isolated from the endophytic fungus Eef-10, namely atraric acid (Compound Ⅰ), ethyl 2, 4-dihydroxy-3, 6-dimethylbenzoate (Compound Ⅱ) and 4-methyl-5, 6-dihydro-2 H-pyran-2-one (Compound Ⅲ). Compound Ⅱ displayed strong inhibitory activities against five test gram-negative bacteria, and the IC₅₀ values were 35.87−55.50 μg/mL. The IC₅₀ values of compound Ⅰ were 67.25−130.55 μg/mL, while compound Ⅲ had IC₅₀ values of more than 200 μg/mL for all five bacteria. The IC₅₀ value of compound Ⅱ for Hep-G2 was 1.50 μg/mL, which was stronger than the positive control camptothecin of 3.6 μg/mL.

Conclusion
Compounds Ⅰ−Ⅲ were isolated from the endophytic fungus Chaetomium sp. Eef-10 and compound Ⅱ showed great antibacterial and antitumor activities on R. solanacearum and Hep-G2 tumor cells.
- endophytic fungus,
- Chaetomium sp. Eef-10,
- antibacterial activity,
- antitumor activity

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References (31)

References

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Identification of endophytic fungus Chaetomium sp. Eef-10 from Eucalyptus exserta and analysis of its active ingredients

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