Metabolites of a symbiotic fungus <i>Aspergillus clavatus</i> XCE02 from <i>Plutella xylostella</i> and their antifungal activities

DING Weijia; LI Mingzhe; CHEN Zelin; CAI Jiachun; ZHANG Zina; LI Yueting; SUN Chenhao; LI Chunyuan

doi:10.7671/j.issn.1001-411X.201904034

Journal of South China Agricultural University > 2020 > 41(2): 76-81. > DOI: 10.7671/j.issn.1001-411X.201904034

DING Weijia, LI Mingzhe, CHEN Zelin, et al. Metabolites of a symbiotic fungus Aspergillus clavatus XCE02 from Plutella xylostella and their antifungal activities[J]. Journal of South China Agricultural University, 2020, 41(2): 76-81. DOI: 10.7671/j.issn.1001-411X.201904034

Citation:

PDF (712 KB)

Metabolites of a symbiotic fungus Aspergillus clavatus XCE02 from Plutella xylostella and their antifungal activities

College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China

More Information

Received Date: April 27, 2019
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To study the metabolites of a symbiotic fungus Aspergillus clavatus XCE02 from Plutella xylostella and their antifungal activities against plant pathogens.

Method
The metabolites were isolated and purified by column chromatography. Their structures were elucidated by spectral analysis. The antifungal activities of the metabolites against Colletotrichum musae and Fusarium graminearum were investigated using paper disc-agar diffusion method.

Result
Ten compounds were isolated and identified as gliomasolide A, Sch725674, gliomasolide C, clavatustide A, clavatustide B, 20-hydroxyergosta-4, 6, 8(14), 22-tetraen-3-one, xanthine, ergosterol, ergosterol peroxide and butanedioic acid. At the concentration of 250 μg/mL, gliomasolide A, Sch725674 and gliomasolide C exhibited high inhibitory activities against C. musae and F. graminearum, clavatustide A and clavatustide B showed moderate inhibitory activities against C.musae and F.graminearum and 20-hydroxyergosta-4, 6, 8(14), 22-tetraen-3-one showed moderate inhibitory activity against F.graminearum.

Conclusion
Gliomasolide A, gliomasolide C and 20-hydroxyergosta-4, 6, 8(14), 22-tetraen-3-one have been obtained from the genus Aspergillus in this study. Gliomasolide A, Sch725674 and gliomasolide C can be used as leading compounds for developing antifungal pesticide.
- insect symbiotic fungus,
- Aspergillus clavatus,
- metabolite,
- antifungal activity,
- column chromatography,
- spectral analysis,
- paper disc-agar diffusion method

FullText(HTML)

References (19)

References

[1]	INTAN SAKINAH M A, SUZIANTI I V, LATIFFAH Z. Phenotypic and molecular characterization of Colletotrichum species associated with anthracnose of banana (Musa spp.) in Malaysia[J]. Genet Mol Res, 2014, 13(2): 3627-3637. doi: 10.4238/2014.May.9.5
[2]	AVOZANI A, REIS E M, TONIN R B. In vitro sensitivity reduction of Fusarium graminearum to DMI and QoIfungicides[J]. Summa Phytopathol, 2014, 40(4): 358-364. doi: 10.1590/0100-5405/1970
[3]	MAHLO S M, MCGAW L J, ELOFF J N. Antifungal activity of leaf extracts from South African trees against plant pathogens[J]. Crop Prot, 2010, 29(12): 1529-1533. doi: 10.1016/j.cropro.2010.08.015
[4]	刘高强, 魏美才. 昆虫资源开发与利用的新进展[J]. 西北林学院学报, 2008, 23(6): 142-146.
[5]	谭周进, 肖启明, 谢丙炎, 等. 昆虫内共生菌研究概况[J]. 微生物学通报, 2005, 32(4): 140-143. doi: 10.3969/j.issn.0253-2654.2005.04.028
[6]	戈惠明, 谭仁祥. 共生菌: 新活性天然产物的重要来源[J]. 化学进展, 2009, 21(1): 30-46.
[7]	马春森, 马罡, 杨和平. 小菜蛾在温带地区越冬研究进展[J]. 生态学报, 2010, 30(13): 3628-3636.
[8]	周丹丽, 徐嘉欣, 朱新伟, 等. 小菜蛾内生菌Bacillus sp.-B03产生的Iturin类代谢产物的研究[C]//中国化学会. 第十一届全国天然有机化学学术会议. 上海: 中国化学会, 2016: 344.
[9]	孙静, 谢淑娜, 刘佳中, 等. 河南省玉米茎基部镰刀菌的形态和分子鉴定[J]. 植物病理学报, 2014, 44(1): 8-16.
[10]	王娜, 钱冠兰, 李晓捷, 等. 海带配子体克隆中一株镰刀菌的分离鉴定[J]. 微生物学通报, 2010, 37(10): 1491-1494.
[11]	郭鹏豪, 刘秀丽, 崔颖鹏, 等. 真菌通用引物ITS1和ITS4在丝状真菌鉴定中的价值评价[J]. 中国微生态学杂志, 2013, 25(8): 922-924.
[12]	李文生, 周丹丽, 陆盈池, 等. 1株红树内生真菌Fusarium sp.代谢产物的研究[J]. 华南农业大学学报, 2017, 38(3): 64-69. doi: 10.7671/j.issn.1001-411X.2017.03.011
[13]	CHEN M, ZHOU J R, LI C Y, et al. Isolation, identification and bioactivity of allele chemicals of Streptomyces sp. strain 6803[J]. Allelopathy J, 2009, 23(2): 411-424.
[14]	章婷, BEDELKHAN A, 郑婕施, 等. 高产洛伐他汀棒曲霉菌株的筛选、鉴定及发酵条件优化[J]. 工业微生物, 2016, 46(6): 24-33. doi: 10.3969/j.issn.1001-6678.2016.06.005
[15]	ZHANG J, LIN X P, LI L C, et al. Gliomasolides A-E, unusual macrolides from a sponge-derived fungus Gliomastix sp. ZSDS1-F7-2[J]. RSC Adv, 2015, 5(67): 54645-54648. doi: 10.1039/C5RA08559D
[16]	YANG S W, CHAN T M, TERRACCIANO J, et al. Structure elucidation of Sch725674 from Aspergillus sp.[J]. J Antibiot, 2005, 58(8): 535-538. doi: 10.1038/ja.2005.74
[17]	JIANG W, YE P, CHEN C, et al. Two novel hepatocellular carcinoma cycle inhibitory cyclodepsipeptides from a hydrothermal vent crab-associated fungus Aspergillus clavatus C2WU[J]. Mar Drugs, 2013, 11(12): 4761-4772. doi: 10.3390/md11124761
[18]	AI H L, ZHANG L M, CHEN Y P, et al. Two new compounds from an endophytic fungus Alternaria solani[J]. J Asian Nat Prod Res, 2012, 14(12): 1144-1148. doi: 10.1080/10286020.2012.733701
[19]	晏小云, 殷晓静, 王国凯, 等. 南苜蓿的化学成分研究[J]. 中国药学杂志, 2012, 47(6): 415-417.

Cited By

Cited by

Periodical cited type(1)

汲蕾，李兆锟，王鹏越，李倩倩，刘为忠，刘德胜. 毛蚶共附生Aspergillus clavatonanicus JL001含氮类次级代谢产物的研究. 中国海洋药物. 2023(05): 65-70 .

Other cited types(3)

Get Citation

PDF

XML

Article views (2414) PDF downloads (2742) Cited by(4)

Turn off MathJax

Article Contents

Abstract

References

Metabolites of a symbiotic fungus Aspergillus clavatus XCE02 from Plutella xylostella and their antifungal activities

Abstract

References

Cited by

Periodical cited type(1)

Other cited types(3)

Catalog

Export File

Citation

Format

Content