Metabolites of a symbiotic fungus <i>Aspergillus clavatus</i> XCE02 from <i>Plutella xylostella</i> and their antifungal activities

DING Weijia; LI Mingzhe; CHEN Zelin; CAI Jiachun; ZHANG Zina; LI Yueting; SUN Chenhao; LI Chunyuan

doi:10.7671/j.issn.1001-411X.201904034

Journal of South China Agricultural University > 2020 > 41(2): 76-81. > DOI: 10.7671/j.issn.1001-411X.201904034

DING Weijia, LI Mingzhe, CHEN Zelin, et al. Metabolites of a symbiotic fungus Aspergillus clavatus XCE02 from Plutella xylostella and their antifungal activities[J]. Journal of South China Agricultural University, 2020, 41(2): 76-81. DOI: 10.7671/j.issn.1001-411X.201904034

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Metabolites of a symbiotic fungus Aspergillus clavatus XCE02 from Plutella xylostella and their antifungal activities

College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China

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Received Date: April 27, 2019
Available Online: May 17, 2023

Abstract

Abstract

Objective
To study the metabolites of a symbiotic fungus Aspergillus clavatus XCE02 from Plutella xylostella and their antifungal activities against plant pathogens.

Method
The metabolites were isolated and purified by column chromatography. Their structures were elucidated by spectral analysis. The antifungal activities of the metabolites against Colletotrichum musae and Fusarium graminearum were investigated using paper disc-agar diffusion method.

Result
Ten compounds were isolated and identified as gliomasolide A, Sch725674, gliomasolide C, clavatustide A, clavatustide B, 20-hydroxyergosta-4, 6, 8(14), 22-tetraen-3-one, xanthine, ergosterol, ergosterol peroxide and butanedioic acid. At the concentration of 250 μg/mL, gliomasolide A, Sch725674 and gliomasolide C exhibited high inhibitory activities against C. musae and F. graminearum, clavatustide A and clavatustide B showed moderate inhibitory activities against C.musae and F.graminearum and 20-hydroxyergosta-4, 6, 8(14), 22-tetraen-3-one showed moderate inhibitory activity against F.graminearum.

Conclusion
Gliomasolide A, gliomasolide C and 20-hydroxyergosta-4, 6, 8(14), 22-tetraen-3-one have been obtained from the genus Aspergillus in this study. Gliomasolide A, Sch725674 and gliomasolide C can be used as leading compounds for developing antifungal pesticide.
- insect symbiotic fungus,
- Aspergillus clavatus,
- metabolite,
- antifungal activity,
- column chromatography,
- spectral analysis,
- paper disc-agar diffusion method

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References (19)

References

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Corresponding author: LI Chunyuan, chunyuanli@scau.edu.cn

College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China

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Metabolites of a symbiotic fungus Aspergillus clavatus XCE02 from Plutella xylostella and their antifungal activities