Effects of nitrogen and phosphorus additions on arbuscular mycorrhizal fungi community in the alpine meadow in Qinghai-Tibetan plateau

LIN Xin; DONG Qiang; WANG Ping; JIANG Lili; HU Jiali; WANG Shiping; JI Baoming

doi:10.7671/j.issn.1001-411X.201903017

Journal of South China Agricultural University > 2020 > 41(2): 95-103. > DOI: 10.7671/j.issn.1001-411X.201903017

LIN Xin, DONG Qiang, WANG Ping, et al. Effects of nitrogen and phosphorus additions on arbuscular mycorrhizal fungi community in the alpine meadow in Qinghai-Tibetan plateau[J]. Journal of South China Agricultural University, 2020, 41(2): 95-103. DOI: 10.7671/j.issn.1001-411X.201903017

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Effects of nitrogen and phosphorus additions on arbuscular mycorrhizal fungi community in the alpine meadow in Qinghai-Tibetan plateau

1.
College of Grassland Science, Beijing Forestry University, Beijing 100083, China
2.
Key Institute of Tibetan Plateau, Chinese Academy of Sciences/Laboratory of Alpine Ecology, Chinese Academy of Sciences, Beijing 100101, China
3.
Center for Excellence in Tibetan Plateau Earth Sciences, Chinese Academy of Sciences, Beijing 100101, China

More Information

Received Date: March 11, 2019
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To investigate the responses of arbuscular mycorrhizal fungi (AMF) community to nitrogen and phosphorus additions and the driven factors in the Qinghai-Tibetan alpine meadow, and have a better knowledge of the effects of fertilization on alpine meadow AMF community.

Method
Through regular analyses and high-throughput sequencing (Illumina Miseq), we studied the effects of nitrogen addition (0, 7.5, 15.0 g·m⁻²) and phosphorus addition (0, 7.5, 15.0 and 30.0 g·m⁻² P₂O₅) for three year on soil chemical property, AMF colonization rate, OTU richness, Shannon diversity index and AMF community composition in Qinghai-Tibetan alpine meadow.

Result
The 36 AMF OTUs from seven families were detected. The addition of nitrogen, phosphorus and their interaction had no influence on AMF colonization rate, OTU richness and Shannon diversity index. Compared with low nitrogen addition treatment, high nitrogen input treatment significantly decreased the relative abundance of Glomeraceae. Soil organic carbon, nitrate nitrogen, available phosphorus and total phosphorus contents were the factors which impacted AMF community.

Conclusion
Root AMF community in Qinghai-Tibetan alpine Kobresia meadow wasn’ t affected by nitrogen or phosphorus addition. However, the distribution of community was significantly correlated with edaphic factors.
- arbuscular mycorrhizal fungi,
- microbial community,
- nitrogen addition,
- phosphorus addition,
- alpine meadow,
- Qinghai-Tibetan plateau

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References (37)

References

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Effects of nitrogen and phosphorus additions on arbuscular mycorrhizal fungi community in the alpine meadow in Qinghai-Tibetan plateau

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