STUDY ON THE PROCEDURE OF WHOLE MOUNT FLUORESCENCE IN SITU HYBRIDIZATION FOR DETECTING SPECIFIC DNA IN RICE

A procedure of whole mount flurescence in situ hybridization (WFISH) for detecting specific DNA in rice was reported. The techique was demonstrated on root and anther of rice with rDNA as probe, labelled by DIG. It was found that the hybridization efficiency depended on the methods further used after fixation, types of tissue and of fixative. Fixation in 1% formalin plus enzyme digestion could yield excellent results of DNA:DNA in root tips. Fixation in 1% formalin (no need enzyme digestion) yielded the excellent results with respect to morphology and hybridization efficiency in rice anther.