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Gao Wentong, Gao Qiaowan, Fan Huaizhong. STUDY ON PURIFICArION OF PAPAYA RINGSPOT VIRUS[J]. Journal of South China Agricultural University, 1996, (1): 15-20.
Citation: Gao Wentong, Gao Qiaowan, Fan Huaizhong. STUDY ON PURIFICArION OF PAPAYA RINGSPOT VIRUS[J]. Journal of South China Agricultural University, 1996, (1): 15-20.
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STUDY ON PURIFICArION OF PAPAYA RINGSPOT VIRUS

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  • Published Date: January 09, 1996
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  • Every bes t cho ice of several main factors affecting the crude purification of p ap ayaringspot virus(PRV)(including kinds of propagation host,organic clarification agent,dis pers io n agent,centrifugal force fo removing sediment before coneentrating thevirus,supereentrifugal foree for concentrating the virus)as reported detailedly in thispaper.Jelly melon(Cu cum is metuliferus)was found to be a better propagation hostthan the Zucchini Squash(Cucurb itapepo).A simple proeedure of one cycle of diffe-rent speed contrifugation and one cycle of Cs_2SO_4 continuous density gradientsupereentrifugation with sugar cushion was designed.The virus preparation showed atypical ultraviolet absorption curve for nucleoproteins,A260/A280=1. 68,the yield ofvirus was 2.56 mg/100 g of infected squash leaves and the virus preparation couldbe suitable for the further research on the serology and molecular biology of PRV.
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