PREPARATION OF MONOCLONAL ANTIBODIES AGA-INST EGG DROP SYNDROME 1976 VIRUS AND THEIR PRELIMINARY CHARACTERIZATION

BLAB/C mice were immunized with chromatographically purified EDS'76 virusV205 strain,and splenocytes were prepared three days after the final boosterimmunization. Fusion of lymphocyte and myeloma SP2/0 cell line was carriedout with the fusogen 50% polyethylene glycol(M.W. 4 000).Five antibodysecreting cel lines,named M2,M3,M10,M14 and M15,were finally obtained afterselective cultivation,specific antibody detection and cloning.It was shown thatM15 monoclonal antibody had haemagglutination inhibition activity with a titerof 6(log2)in microplate HI test.All five McAbs were determined to be stronglyspecific to EDS'76 virus with no reaction to IBV,ILTV,influenza A virus,CELOvirus,NDV and IBDV.