STUDIES ON THE PREVENTION OF VITRIFICATION OF IN VITRO CULTURE OF CARNATION BY USING ETHYLENE RELEASERS

Experiments were carried out with carnation by using ethylene releasers as growth regulators suppiemellted in the media. Results showed that ACC, the immediate precursor of ethylene, exhibited significant effect on preventing the vitrification of in vitro culture of carnation, and obtaining full - grown plantlets without subculture. The induction frequency of normal planllets was 98%. A compact stem and leaf and good root system was observd in in vitro culture. Under microscopical examination, closely arranged cells and well developed sclerenchyma tissue were found in cross sections of the stem. Biochemical analysis revealed that the dry matter and chlorophyll contents were higher than those of vitrified ones by 1.7 and 5. 5 times, respectively. The growth of plantlets induced by CEPA were similar to those by ACC. The quantity of ethylene released in the culture tubes where the media was supplemented with ACC or CEPA was found to be much higher than that in the control lubes, implying that ethylene had good effect on vitrification prevention.