| Citation: |
CHEN Huilin, WU Yutong, GAO Jie, et al. Resistance to beta cypermethrin and its metabolic pathway in Spodoptera frugiperda population in Sichuan[J]. Journal of South China Agricultural University, 2025, 46(4): 1-11. DOI: 10.7671/j.issn.1001-411X.202501008
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To investigate the resistance and metabolic pathways of beta cypermethrin in fall armyworm (Spodoptera frugiperda) populations in Sichuan.
The resistance levels of fall armyworm to beta cypermethrin in MY, DC, CX, HD and RH populations in Sichuan were determined by drip method. The activities and gene expressions of three kinds of detoxification metabolic enzymes, such as cytochrome P450 enzymes (P450s), carboxylesterases (CarE) and glutathione S-transferase (GST), were determined by photoelectric colorimetric method and RT-qPCR. The Pearson correlation coefficient was used to analyze the correlation of beta cypermethrin resistance with enzyme activity and gene expressions, and the differences in beta cypermethrin metabolites were analyzed by liquid chromatography-mass spectrometry (LC-MS).
The MY population showed the highest relative resistance ratio (RR) to beta cypermethrin, which was 4.02 times, and the LD50 value was 84.201 μg/g. The activities of CarE, GST and P450s increased with the increase of beta cypermethrin resistance, and the expression levels of CES12, GST epsilon9 and CYP6B50 genes were significantly up-regulated and significantly correlated with beta cypermethrin resistance. The metabolism differential products in the control group (MY) and the experimental group (MY + LD50 beta cypermethrin) were 3-phenoxybenzoic acid (3-PBA), pyro catechol, capric acid, methyl-2, 3-dihydro-3, 5-dihydroxy-2-oxo-3-indoleacetic acid. Among them, 3-PBA was only detected in the experimental group (MY + LD50 beta cypermethrin) and not in the control group (MY). It was inferred that beta cypermethrin was metabolized into 3-PBA through the enzyme activity of fall armyworm, and then pyro catechol was finally generated through oxidation and hydrolysis.
Spodoptera frugiperda may up-regulate the expression of genes associated with detoxification enzymes, leading to increased enzyme activities. This enhanced detoxification capacity accelerates the metabolic degradation of lamb da-cyhalo thrin, thereby reducing the pesticide’s efficacy.
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