Effect of complex microbial inoculation on growth, and nitrogen and phosphorus uptake of maize and soybean in intercropping system

HUANG Huozhi; LIU Yang; CHEN Kang; WANG Xiurong

doi:10.7671/j.issn.1001-411X.202412008

Journal of South China Agricultural University > 2025 > Uncorrected proof > DOI: 10.7671/j.issn.1001-411X.202412008

HUANG Huozhi, LIU Yang, CHEN Kang, et al. Effect of complex microbial inoculation on growth, and nitrogen and phosphorus uptake of maize and soybean in intercropping system[J]. Journal of South China Agricultural University, 2025, 46(5): 1-12. DOI: 10.7671/j.issn.1001-411X.202412008

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Effect of complex microbial inoculation on growth, and nitrogen and phosphorus uptake of maize and soybean in intercropping system

Root Biology Center, South China Agricultural University/State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangzhou 510642, China

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Abstract

Abstract

Objective
To investigate the effects of inoculation with synthetic microbial communities on growth, and nitrogen (N) and phosphorus (P) uptake of monocultured or intercropped maize and soybean, and provide a theoretical basis for utilizing efficiently microbial inoculants to enhance crop yield.
Method
Using unsterilized soil in monoculture and intercropping systems of maize and soybean, a pot experiment, including eight treatments of non-inoculated control (CK), single inoculation with arbuscular mycorrhizal (AM) fungi (A), rhizobia (R) or Bacillus (B), dual inoculation with AM fungi and rhizobia (A+R), AM fungi and Bacillus (A+B), or rhizobia and Bacillus (R+B), and triple inoculation with AM fungi, rhizobia and Bacillus (A+R+B), was conducted to determine plant dry weight, N and P contents, root traits, mycorrhizal colonization rate, nodule traits, pH and acid phosphatase activity in the rhizosphere.
Result
The different combinations of dual inoculation exhibited better inoculation effects. Compared with CK, dual inoculations of A+B and R+B increased plant dry weight by 14.11% and 13.31%, N content by 30.02% and 20.56%, and P content by 17.77% and 16.84% in the monocultured maize, respectively. Dual inoculation of R+B increased plant dry weight, N and P contents by 28.53%, 33.55% and 17.29% in the monocultured soybean, respectively. Compared with dual inoculation of A+B, plant dry weight of the monocultured or intercropped maize with triple inoculation of A+R+B significantly reduced. Compared with dual inoculation of R+B, plant dry weight and N and P contents of the monocultured soybean with triple inoculation of A+R+B also significantly reduced. Intercropping significantly increased plant dry weight and N and P contents of maize (except for the A+R+B treatment); Dual inoculation of R+B promoted N and P uptake and plant growth of the intercropped soybean, and partially alleviated the inhibitory effect of intercropping on soybean growth. Compared with CK, Dual inoculation of R+B promoted mycorrhizal colonization rate of the intercropped maize and nodulation of monocultured or intercropped soybean plant. Additionally, dual inoculation of A+B and R+B, as well as intercropping promoted maize root growth, and altered rhizosphere processes of both maize and soybean, thereby enhanced crop acquisition of N and P.
Conclusion
In the complex environment of soil, single inoculation has limited effectiveness, while dual inoculations with complex microbial communities and intercropping play a significant role in improving N and P acquisition and promoting maize growth.
- Maize,
- Soybean,
- Nitrogen and phosphorus uptake,
- Intercropping,
- complex microbial community,
- Crop growth,
- Microbial agent

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References (48)

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