Study on the disease suppression mechanism of Bacillus halotolerans against Colletotrichum siamense

ZHU Youjiao; CHEN Jianxin; WU Fengjinglin; PENG Ruiqi; WEN Mingjia; MA Huancheng; WU Jianrong

doi:10.7671/j.issn.1001-411X.202411023

Journal of South China Agricultural University > 2025 > Uncorrected proof > DOI: 10.7671/j.issn.1001-411X.202411023

ZHU Youjiao, CHEN Jianxin, WU Fengjinglin, et al. Study on the disease suppression mechanism of Bacillus halotolerans against Colletotrichum siamense[J]. Journal of South China Agricultural University, 2025, 46(4): 1-10. DOI: 10.7671/j.issn.1001-411X.202411023

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Study on the disease suppression mechanism of Bacillus halotolerans against Colletotrichum siamense

1.
Key Laboratory of Forest Disaster Warning and Control in Universities of Yunnan Province/College of Forestry, Southwest Forestry University, Kunming 650224, China
2.
Key Laboratory of Biodiversity Conservation in Southwest China State Forestry Administration/College of Forestry, Southwest Forestry University, Kunming 650224, China

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Abstract

Abstract

Objective
To analyze the disease prevention mechanisms of endophytic bacteria against Colletotrichum siamense causing camellia anthracnose.
Method
The inhibitory effect of the endophytic bacterial strain B11 on C. siamense was evaluated using the dual-culture method. The disease-suppressing and induced resistance mechanisms of the endophytic bacteria were investigated through scanning electron microscopy, amplification of compound synthesis genes and RT-qPCR.
Result
Based on morphological characteristics and multi-gene phylogenetic analysis, the endophytic bacterial strain B11 was identified as Bacillus halotolerans. This strain exhibited broad-spectrum in vitro antimicrobial activity, with significant inhibitory effects on C. siamense. Its fermentation broth caused disruption of the cell wall of C. siamense strain CA17, leakage of intracellular contents and abnormal swelling of hyphae. Additionally, after treatment with the fermentation broth of strain B11, the marker genes PR1 and PR5 in the salicylic acid (SA) signaling pathway were upregulated in Arabidopsis leaves. Following treatment of Camellia leaves with the fermentation broth, the activities of defense-related enzymes POD and SOD were significantly increased.
Conclusion
This study provides important theoretical support for the research and development of green prevention and control technologies for C. siamense and the development and application of biological control agents.
- Colletotrichum siamense,
- Endophytic bacteria,
- Bacillus halotolerans,
- Biological control

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References

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Study on the disease suppression mechanism of Bacillus halotolerans against Colletotrichum siamense

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