Study on pollen vigor of <i>Schima superba</i> under different storage conditions

YE Jingxin; NING Huagong; ZHONG Zizheng; ZHANG Fangyou; HE Qian; LIU Tianyi

doi:10.7671/j.issn.1001-411X.202410017

Journal of South China Agricultural University > 2025 > Uncorrected proof > DOI: 10.7671/j.issn.1001-411X.202410017

YE Jingxin, NING Huagong, ZHONG Zizheng, et al. Study on pollen vigor of Schima superba under different storage conditions[J]. Journal of South China Agricultural University, 2025, 46(4): 1-8. DOI: 10.7671/j.issn.1001-411X.202410017

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Study on pollen vigor of Schima superba under different storage conditions

1.
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
2.
Yingde Institute of Forestry Science, Qingyuan 513055, China

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Graphical Abstract

Abstract

Abstract

Objective
In order to explore the best preservation conditions of Schima superba pollen, an efficient storage system of S. superba pollen was constructed.
Method
In this study, 9-year-old S. superba individual plants were used as the research object, and pollen collection was carried out at different flowering stages. The changes of pollen vigor under different collection time, pollen vigor detection methods and different storage conditions were analyzed.
Result
The flower branches at different flower bud stages were hydroponically cultured. The flowers opened on the first day had high pollen vigor and no significant difference. It was appropriate to collect pollen from 10:00 to 12:00 in the morning. The TTC staining method was the most suitable for rapid identification of S. superba pollen vigor, and the in vitro germination method was more accurate. The pollen germination effect was the best when the in vitro germination medium was 0.1 g·L⁻¹ H₃BO₃ + 100 g·L⁻¹ sucrose. Under the same storage time, reducing the storage temperature can effectively slow down the decline rate of pollen vigor, and different temperatures had a significant effect on the pollen vigor. When short-term storage was required, the low temperature condition of 4 ℃ could ensure that the pollen could maintain its vitality within one month and complete the cross-pollination work of the year. For long-term storage, the −20 °C and −80 ℃ storage conditions are better.
Conclusion
The results of this study not only provide a scientific basis for the collection and storage of S. superba pollen, but also provide a reference for solving the problem of artificial hybridization breeding caused by inconsistent flowering periods in seed orchards.
- Schima superba,
- Pollen vigor,
- Low temperature storage,
- Pollen collection

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References (41)

References

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