Effect of initiation codon mutation within <i>tva</i> gene on Qingyuan partridge chicken resistance to infection by avian leukemia virus subgroup K

XU Huijuan; KUANG Zhixiang; ZUO Kejing; WU Chengyang; ZHENG Honghao; XIE Qingmei; CHEN Weiguo

doi:10.7671/j.issn.1001-411X.202408023

Journal of South China Agricultural University > 2025 > Corrected proof > DOI: 10.7671/j.issn.1001-411X.202408023

XU Huijuan, KUANG Zhixiang, ZUO Kejing, et al. Effect of initiation codon mutation within tva gene on Qingyuan partridge chicken resistance to infection by avian leukemia virus subgroup K[J]. Journal of South China Agricultural University, 2025, 46(3): 1-7. DOI: 10.7671/j.issn.1001-411X.202408023

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Effect of initiation codon mutation within tva gene on Qingyuan partridge chicken resistance to infection by avian leukemia virus subgroup K

1.
College of Animal Science, South China Agricultural University/Guangdong Provincial Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, China
2.
Guangdong Love-Health Biotechnology Co., Ltd., Qingyuan 511800, China
3.
Guangzhou Zoo/Guangzhou Wildlife Research Center, Guangzhou 510070, China

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Abstract

Abstract

Objective
To investigate the effect of initiation codon mutation within tva receptor gene (tva c.3G>A) on Qingyuan partridge chicken resistance to infection by avian leukosis virus subgroup K (ALV-K).
Method
The tva c.3G>A mutation site within Qingyuan partridge chicken lines was genotyped by using direct sequencing method. The infection of RCASBP(K)-EGFP fluorescent reporter virus and ALV-K GD1601 virus to chicken embryo fibroblasts (CEFs) with different genotypes of tva c.3G>A mutation site were detected by flow cytometry and RT-qPCR, respectively. The infection of ALV-K GD1601 virus to Qingyuan partridge chicks with different genotypes of tva c.3G>A mutation site were detected by RT-qPCR.
Result
The genotyping results of tva c.3G>A mutation site showed that the heterozygous mutant genotype tva c.3G>A and the homozygous mutant genotype tva c.3A/A existed in Qingyuan partridge chicken lines K and M, with the frequencies of 0.183, 0.133 for tva c.3G/A and 0.116, 0.033 for tva c.3A/A, respectively. The results of flow cytometry and RT-qPCR showed that the tva c.3G>A mutation site wild-type tva c.3G/G CEFs were susceptible to infection by both RCASBP(K)-EGFP and ALV-K GD1601 virus, while the homozygous mutant genetype tva c.3A/A CEFs were effectively resistant to infection by RCASBP(K)-EGFP and ALV-K GD1601 viruses, demonstating that tva c.3G>A mutation caused Qingyuan partridge chicken resistance to infection by ALV-K in vitro. The results of ALV-K challenge test indicated that tva c.3G>A mutation led to Qingyuan partridge chicks resistance to infection by ALV-K in vivo.
Conclusion
The tva c.3G>A mutation confers Qingyuan partridge chicken resistance to infection by ALV-K both in vitro and in vivo, indicating that it can be used as a genetic marker for ALV-K resistance selection in Qingyuan partridge chicken.
- Qingyuan partridge chicken,
- Avian leukosis virus subgroup K,
- tva receptor gene,
- Mutation,
- Genetic resistance

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References

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Effect of initiation codon mutation within tva gene on Qingyuan partridge chicken resistance to infection by avian leukemia virus subgroup K

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