Effects of altitude on gut structure, digestive enzyme activity and gut microbiota of <i>Cyprinus carpio</i> var. <i>Jinbei</i> in rice field

YANG Li; MENG Qingmi; BAI Tianquan; LI Jiayao; YAO Junjie; MA Lan

doi:10.7671/j.issn.1001-411X.202406044

Journal of South China Agricultural University > 2024 > 45(6): 898-907. > DOI: 10.7671/j.issn.1001-411X.202406044

YANG Li, MENG Qingmi, BAI Tianquan, et al. Effects of altitude on gut structure, digestive enzyme activity and gut microbiota of Cyprinus carpio var. Jinbei in rice field[J]. Journal of South China Agricultural University, 2024, 45(6): 898-907. DOI: 10.7671/j.issn.1001-411X.202406044

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Effects of altitude on gut structure, digestive enzyme activity and gut microbiota of Cyprinus carpio var. Jinbei in rice field

1.
Fishery Resources and Environment Research Center, Guizhou University/Key Laboratory of Genetic Breeding and Reproduction of Highland Mountain Animals, Ministry of Education, Guiyang 550025, China
2.
Dushan County Aquaculture Development Center, Dushan 558200, China
3.
Key Laboratory of Rice-Fishery Integrated Breeding and Ecology, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China

More Information

Received Date: June 26, 2024
Available Online: September 18, 2024
Published Date: September 17, 2024

Graphical Abstract

Abstract

Abstract

Objective
In order to explore the gut structure, digestive enzyme activity and gut microbiota characteristics of Cyprinus carpio var. Jinbei at different altitudes.
Method
C. carpio var. Jinbei in rice fields at low (580 m), middle (830 m) and high (1340 m) altitudes were taken as the research objects. The histology, enzymology and molecular biology methods were used to compare and analyze the gut tissue structure, digestive enzyme activity and gut microbiota characteristics.
Result
The villus height of C. carpio var. Jinbei of the low altitude group (521.04 μm) was significantly higher than those of the middle altitude group (438.01 μm) and high altitude group (419.39 μm) (P< 0.05). The trypsin activity of C. carpio var. Jinbei of the middle altitude group (4541.65 U/mg) was significantly higher than those of the low altitude group (4023.72 U/mg) and high altitude group (3722.10 U/mg) (P<0.05). Actinobacteria, Firmicutes, Proteobacteria, Chloroflexi and Tenericutes were the main gut microbiota of C. carpio var. Jinbei in rice field.
Conclusion
Different altitudes affect the intestinal morphology, digestive enzyme activity, and gut microbiota structure of C. carpio var. Jinbei in rice field. Although the gut microbiota of C. carpio var. Jinbei in rice field changes at different altitudes, it still maintains a relatively stable gut microbiota dominated by Actinobacteria, indicating that C. carpio var. Jinbei gut has been adapted to the ecological environment in rice field.
- Altitude,
- Rice field,
- Cyprinus carpio var. Jinbei,
- Intestine,
- Digestive enzyme,
- Gut microbiota

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References (36)

References

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