Effects of increasing ditches and pits in rice field on intestinal structure, digestive enzyme activity and microbial community of <i>Cyprinus carpio</i> var. <i>Jinbei</i>

MA Lan; MENG Qingmi; LI Jiayao; QIN Guobing; YAO Junjie; WANG Xiulong; YANG Li

doi:10.7671/j.issn.1001-411X.202405036

Journal of South China Agricultural University > 2024 > 45(6): 889-897. > DOI: 10.7671/j.issn.1001-411X.202405036

MA Lan, MENG Qingmi, LI Jiayao, et al. Effects of increasing ditches and pits in rice field on intestinal structure, digestive enzyme activity and microbial community of Cyprinus carpio var. Jinbei[J]. Journal of South China Agricultural University, 2024, 45(6): 889-897. DOI: 10.7671/j.issn.1001-411X.202405036

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Effects of increasing ditches and pits in rice field on intestinal structure, digestive enzyme activity and microbial community of Cyprinus carpio var. Jinbei

1.
Fishery Resources and Environment Research Center/Key Laboratory of Genetic Breeding and Reproduction of Highland Mountain Animals, Ministry of Education, Guizhou University, Guiyang 550025, China
2.
Key Laboratory of Rice-Fishery Integrated Breeding and Ecology, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China
3.
Guiyang Fisheries Station, Guiyang 550081, China

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Received Date: May 26, 2024
Available Online: August 20, 2024
Published Date: August 22, 2024

Graphical Abstract

Abstract

Abstract

Objective
To explore the rice field adaptive characteristics of Cyprinus carpio var. Jinbei (goldenback carp), as well as the relationship between their gut and different rice field environments.
Method
The study focused on the growth of goldenback carp in flat plate, “single line” ditch, and “cross-shaped” ditch rice fields, using morphological, enzymatic and bioinformatics methods to investigate the effects of adding ditches and pits in rice fields on the intestinal structure, digestive enzyme activities and microbial communities of the goldenback carp.
Result
The villus width of goldenback carp intestine in “cross-shaped” ditch group was the widest (173.59 μm), followed by the “single line” ditch group (157.72 μm), and the flat plate group was the narrowest (139.69 μm). The trypsin activities of the “cross-shaped” ditch group and the “single line” ditch group were 4 662.65 and 4 676.12 U·mg⁻¹ respectively, significantly higher than that of the flat plate group (3 752.34 U·mg⁻¹) (P<0.05). At the phylum level, after adding ditches and pits in rice fields, Proteobacteria and Firmicutes remained dominant, but Actinobacteria replaced the Bacteroidetes as the dominant phylum. At the genus level, the dominant genera in the flat plate group were Streptococcus, Cetobacterium and Rhodobacter. There were obvious changes in the dominant genera of the “single line” ditch and “cross-shaped” ditch groups, among the top three dominant bacterial genera in each group, one changed in the “single line” ditch, and two changed in the “cross-shaped” ditch.
Conclusion
The intestinal structure, digestive enzyme activity, microbial community and dominant bacterial groups of the goldenback carp change with the addition of ditches and pits in rice fields, regulating metabolism to adapt to the new rice field water environments. Despite the changes, the composition of the core intestinal flora of the goldenback carp remains relatively stable.
- Rice field,
- Ditch and pit,
- Cyprinus carpio var. Jinbei,
- Intestinal structure,
- Digestive enzyme,
- Microbial community

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References (25)

References

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Effects of increasing ditches and pits in rice field on intestinal structure, digestive enzyme activity and microbial community of Cyprinus carpio var. Jinbei

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