Effect of feeding level on feeding ecology of <i>Pelodiscus sinensis</i> cultured in rice field

WU Benli; HUANG Long; WU Cangcang; CHEN Jing; CHEN Xiajun; MEI Yaoping; HE Jixiang

doi:10.7671/j.issn.1001-411X.202405028

Journal of South China Agricultural University > 2024 > 45(6): 929-938. > DOI: 10.7671/j.issn.1001-411X.202405028

WU Benli, HUANG Long, WU Cangcang, et al. Effect of feeding level on feeding ecology of Pelodiscus sinensis cultured in rice field[J]. Journal of South China Agricultural University, 2024, 45(6): 929-938. DOI: 10.7671/j.issn.1001-411X.202405028

Citation:

PDF (915 KB)

Effect of feeding level on feeding ecology of Pelodiscus sinensis cultured in rice field

Anhui Key Laboratory of Aquaculture and Stock Enhancement/Fishery Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China

More Information

Received Date: May 16, 2024
Available Online: September 22, 2024
Published Date: September 17, 2024

Graphical Abstract

Abstract

Abstract

Objective
The purpose of this study was to investigate the species and abundance of natural food for Chinese soft-shelled turtles (Pelodiscus sinensis) cultured in paddy fields under different feeding levels, explore the feeding ecology of P. sinensis in the rice-turtle co-culture mode, and provide a basis for optimizing the feeding strategy of comprehensive planting and breeding in rice fields and revealing the formation process and mechanism of ecological benefits.
Method
The field feeding experiment was conducted with three feeding levels of 0.7%, 1.4% and 2.1%(w) for studying rice-turtle co-culture. Morphological analysis of stomach contents and environmental DNA analysis were conducted to determine the feeding habit of the natural food and commercial feeds for P. sinensis in different treatment groups. The relative importance index of different foods in treatments was also analyzed.
Result
The feeding rate of P. sinensis increased with the increase of feeding level. In morphological analysis of stomach contents, 8 species of fish, 2 species of shrimp, 2 species of molluscs, 9 species of insects and rotifers were identified. The species with high occurrence rate included Abbottina rivularis, Pseudorasbora parva, Macrobrachium nipponense, Cipangopaludina chinensis, Polyarthra sp., Chironomidae larvae and Limnodrilus sp.. The occurrence rate and relative importance index proportion (IRIP) of commercial feeds increased with the increase of feeding level, while the IRIP of natural food decreased. The IRIP of commercial feeds was 91.97% when the feeding level was 2.1%, and the added IRIP of multiple natural foods was only 8.03%. In environmental DNA analysis, there were 16 species of fish with DNA relative abundance higher than 0.5%, and among them, A. rivularis, P. parva, Rhodeus sinensis, Hemiculter tchangi, Misgurnus anguillicaudatus, Paramisgurnus dabryanus, Carassius auratus and Hemisalanx brachyrostralis were also identified in morphology analysis of stomach contents. A total of 36 species of large aquatic vertebrates were identified, and the species with DNA relative abundance higher than 1.0% in stomach content samples mainly included Pseudochaeta sp. Janzen14, Palaemom modestus, Cipangopaludina chinensis, Chironomidae larvae, Limnodrilus sp., rotifer, etc.
Conclusion
The natural food of P. sinensis includes small fish, molluscs, arthropods and rotifers, etc. Higher feeding level of commercial feeds results in lower feeding rate and IRIP of natural food, which may reduce the ecological benefits of co-culture.
- Rice-turtle co-culture,
- Feeding level,
- Feeding ecology,
- Stomach content,
- Environmental DNA

FullText(HTML)

References (38)

References

[1]	于秀娟, 郝向举, 党子乔, 等. 中国稻渔综合种养产业发展报告(2023)[J]. 中国水产, 2023, 573(8): 19-26.
[2]	WANG C, SHI X, QI Z, et al. How does rice-animal co-culture system affect rice yield and greenhouse gas? A meta-analysis[J]. Plant and Soil, 2023, 493: 325-340. doi: 10.1007/s11104-023-06233-x
[3]	何志刚. 稻渔综合种养生态系统中生物多样性, 营养与食物[J]. 湖南饲料, 2018(4): 13-17. doi: 10.3969/j.issn.1673-7539.2018.04.006
[4]	SIG H, PENG C L, YUAN J F, et al. Changes in soil microbial community composition and organic carbon fractions in an integrated rice–crayfish farming system in subtropical China[J]. Scientific Reports, 2017, 7: 2856. doi: 10.1038/s41598-017-02984-7
[5]	刘其根, 李丰, 罗衡, 等. 引入养殖鳖对稻田土壤固氮菌的影响[J]. 应用与环境生物学报, 2018, 24: 483-492.
[6]	罗衡, 赵良杰, 李丰, 等. 养殖鳖的引入对稻田土壤细菌群落结构的影响[J]. 水产学报, 2018, 42: 720-732.
[7]	CAI S M, LV W G, ZHU H T, et al. Effect of nitrogen application rate on soil fungi community structure in a rice-fish mutualistic system[J]. Scientific Reports, 2019, 9: 16188. doi: 10.1038/s41598-019-52602-x
[8]	HU L L, REN W Z, TANG J J, et al. The productivity of traditional rice-fish co-culture can be increased without increasing nitrogen loss to the environment[J]. Agriculture, Ecosystems & Environment, 2013, 177: 28-34.
[9]	KOIZUMI N, MORI A, MINETA T, et al. Plant species identification using fecal DNAs from red-eared slider and Reeves’ pond turtle in agricultural canals for rural ecosystem conservation[J]. Paddy and Water Environment, 2017, 15(4): 723-730. doi: 10.1007/s10333-016-0576-5
[10]	盛瀛. 稻鳖共作对稻飞虱的防控效果[J]. 浙江农业科学, 2019, 60(6): 933-934.
[11]	WAN N F, LI S X, LI T, et al. Ecological intensification of rice production through rice-fish co-culture[J]. Journal of Cleaner Production, 2019, 234: 1002-1012. doi: 10.1016/j.jclepro.2019.06.238
[12]	XIE J, HU L L, TANG J J, et al. Ecological mechanisms underlying the sustainability of the agricultural heritage rice-fish coculture system[J]. Proceedings of the National Academy of Sciences of the United States of America, 2011, 108(50): 1381-1387.
[13]	GE L, SUN Y, LI Y, et al. Ecosystem sustainability of rice and aquatic animal co-culture systems and a synthesis of its underlying mechanisms[J]. Science of the Total Environment, 2023, 880: 163314. doi: 10.1016/j.scitotenv.2023.163314
[14]	梁玉刚. 垄作稻鱼鸡共生系统水稻产量形成及其生态学效应研究[D]. 长沙: 湖南农业大学, 2020.
[15]	SUN C X, ZHANG D D, LIU W B, et al. Growth performance, digestion and metabolism to fish meal replacement by rice protein concentrate in Chinese soft-shelled turtle Pelodiscus sinensis[J]. Aquaculture, 2018, 492: 321-326. doi: 10.1016/j.aquaculture.2018.04.032
[16]	ZHANG J, HU L, REN W, et al. Rice-soft shell turtle coculture effects on yield and its environment[J]. Agriculture, Ecosystems & Environment, 2016, 224: 116-122.
[17]	GUO L, ZHAO L F, YE J L, et al. Using aquatic animals as partners to increase yield and maintain soil nitrogen in the paddy ecosystems[J]. eLife, 2022, 11: 73869. doi: 10.7554/eLife.73869
[18]	ZHAO J, ZOU Y, CHEN H, et al. Effects of stocking density of Chinese soft-shelled turtle and interactions between cultivated species on growth performance and the environment in a turtle-rice coculture system[J]. Journal of the World Aquaculture Society, 2020, 51(3): 788-803. doi: 10.1111/jwas.12665
[19]	WU B L, CHEN J, HUANG L, et al. Dynamics of soil fertility and microbial community response to stocking density in rice-turtle co-culture[J]. Aquaculture Reports, 2021, 20: 100765. doi: 10.1016/j.aqrep.2021.100765
[20]	WU B L, HUANG L, WU C C, et al. Comparative analysis of the growth, physiological responses, and gene expression of Chinese soft-shelled turtles cultured in different modes[J]. Animals, 2024, 14(6): 962. doi: 10.3390/ani14060962
[21]	ASSAN D, HUANG Y, MUSTAPHA U F, et al. Fish feed intake, feeding behavior, and the physiological response of apelin to fasting and refeeding[J]. Frontiers in Endocrinology, 2021, 12: 798903. doi: 10.3389/fendo.2021.798903
[22]	KAUSSHIK S J. Feed management and on-farm feeding practices of temperate fish with special reference to salmonids[J]. FAO Fisheries and Aquaculture Technical Paper, 2013, 583: 519-551.
[23]	LUO L, LI T L, XING W, et al. Effects of feeding rates and feeding frequency on the growth performances of juvenile hybrid sturgeon, Acipenser schrenckii Brandt♀×A. baeri Brandt♂[J]. Aquaculture, 2015, 448: 229-233. doi: 10.1016/j.aquaculture.2015.06.005
[24]	李笑天, 刘宝良, 费凡, 等. 投喂策略对水产动物生长生理及行为特征影响研究进展[J]. 渔业现代化, 2020, 47(2): 7-15. doi: 10.3969/j.issn.1007-9580.2020.02.002
[25]	魏凤. 秸秆还田和投食对稻虾共作系统小龙虾食源和水生生物多样性的影响[D]. 武汉: 华中农业大学, 2023.
[26]	PARK J C, KODAMA K, OYAMA M, et al. Changes in feeding habits of the starspotted smooth-hound, Mustelus manazo, in Tokyo Bay between periods with different stock size levels[J]. Marine Pollution Bulletin, 2020, 152: 110863. doi: 10.1016/j.marpolbul.2019.110863
[27]	HU Y, YANG T, LIU Y, et al. High fish stocking density weakens the effects of rice-fish co-culture on water eutrophication and greenhouse gas emissions[J]. Water, Air, & Soil Pollution, 2022, 233(6): 222.
[28]	周勇, 李皓轩, 董洪瑞, 等. 基于小龙虾田间分布的稻虾共生机制探究[J]. 华中农业大学学报, 2022, 41(4): 202-209. doi: 10.3969/j.issn.1000-2421.2022.4.hznydx202204026
[29]	刘雯, 李娜, 鞠民, 等. 在不同稻渔共作模式下中小型土壤动物群落特征研究[J]. 江西师范大学学报(自然科学版), 2021, 45(2): 188-193.
[30]	ZHANG K, PENG H H, XIA Y, et al. Evaluating ecological mechanisms and optimization strategy of rice-fish co-culture system by ecosystem approach[J]. Aquaculture, 2022, 560: 738561. doi: 10.1016/j.aquaculture.2022.738561
[31]	DUAN M C, HU W H, LIU Y H, et al. The influence of landscape alterations on changes in ground beetle (Carabidae) and spider (Araneae) functional groups between 1995 and 2013 in an urban fringe of China[J]. Science of the Total Environment, 2019, 689: 516-525. doi: 10.1016/j.scitotenv.2019.06.198
[32]	申祺, 李蔚然, 于小彭, 等. 稻蟹共作对稻田生物影响的研究进展[J]. 北方水稻, 2021, 51(5): 59-62. doi: 10.3969/j.issn.1673-6737.2021.05.019
[33]	桑海旭, 马晓慧, 王井士, 等. 稻蟹共养对水稻有害生物控制效果评价[J]. 北方水稻, 2021, 51(2): 17-20. doi: 10.3969/j.issn.1673-6737.2021.02.004
[34]	蔡羽娟. 江汉平原稻农施药次数的影响因素及其对稻田节肢动物的影响[D]. 武汉: 华中农业大学, 2023.
[35]	陈炼, 吴琳, 刘燕, 等. 环境DNA metabarcoding及其在生态学研究中的应用[J]. 生态学报, 2016, 36(15): 4573-4582.
[36]	ZHANG S, ZHENG Y T, ZHAN A B, et al. Environmental DNA captures native and non-native fish community variations across the lentic and lotic systems of a megacity[J]. Science Advances, 2022, 8(6): eabk0097. doi: 10.1126/sciadv.abk0097
[37]	MÉHEUST E, ALFONSI E, LE MÉNEC P, et al. DNA-barcoding for the identification of soft remains of prey in the stomach contents of grey seals (Halichoerus grypus) and harbour porpoises (Phocoena phocoena)[J]. Marine Biology Research, 2015, 11(4): 385-395. doi: 10.1080/17451000.2014.943240
[38]	MORAN Z, ORTH D J, SCHMITT J D, et al. Effectiveness of DNA barcoding for identifying piscine prey items in stomach contents of piscivorous catfishes[J]. Environmental Biology of Fishes, 2016, 99(1): 161-167. doi: 10.1007/s10641-015-0448-7

[1]	WU Liji, HU Fei. Rainfall characteristics of growth duration of early and late rice in South China[J]. Journal of South China Agricultural University, 2019, 40(1): 1-7. DOI: 10.7671/j.issn.1001-411X.201804034
[2]	ZHOU Duo'en, LIU Dewu, LIAO Yingxin, LIU Guangbin. Study on adaptabilities of Hu sheep and Chuanzhong black goat in Southern China[J]. Journal of South China Agricultural University, 2016, 37(5): 19-23. DOI: 10.7671/j.issn.1001-411X.2016.05.004
[3]	CHENG Yanbo, MA Qibin, MU Yinghui, TAN Zhiyuan, WU Hong, NIAN Hai. Resistance evaluation of soybean varieties to phytophthora root rot in South China[J]. Journal of South China Agricultural University, 2015, 36(4): 69-75. DOI: 10.7671/j.issn.1001-411X.2015.04.013
[4]	ZHAO Yunyun, ZHONG Caixia, FANG Xiaolong, HUANG Yi’an, MA Qibin, NIAN Hai, YANG Cunyi. Genotypic differences of cadmium tolerance among spring-sowing soybean varieties in South China[J]. Journal of South China Agricultural University, 2014, 35(3): 111-113. DOI: 10.7671/j.issn.1001-411X.2014.03.020
[5]	LIU Chuan-guang, ZHOU Xin-qiao, CHEN Da-gang, ZHOU Han-qin, FENG Dao-ji, LI Li-jun, LI Ju-chang, ZHANG Gui-quan, CHEN You-ding. Analysis of Coefficient of Parentage Among Major Commercial Inbred Indica Rice Cultivars in South China[J]. Journal of South China Agricultural University, 2012, 33(3): 277-281. DOI: 10.7671/j.issn.1001-411X.2012.03.002
[6]	ZHANG Yu-ping,ZENG Ling,LU Yong-yue,LIANG Guang-wen. Monitoring of Insecticide Resistance of Bactrocera dorsalis Adults in South China[J]. Journal of South China Agricultural University, 2007, 28(3): 20-23. DOI: 10.7671/j.issn.1001-411X.2007.03.005
[7]	Journal of South China Agricultural University[J]. Journal of South China Agricultural University, 2006, 27(4): F0003-F0003.
[8]	Cloning and Sequencing of HN Gene of NDV WS99 Strain Isolated in South China[J]. Journal of South China Agricultural University, 2001, 22(4): 75-77. DOI: 10.7671/j.issn.1001-411X.2001.04.021
[9]	XIAO Hong-sheng,JI Xian-hua,ZHAO Yu-guang,LUO Fu-he,CHEN Yi-gang. The Application of GPS in South China Forest[J]. Journal of South China Agricultural University, 2000, (2): 5-7. DOI: 10.7671/j.issn.1001-411X.2000.02.002
[10]	Wu Zhimin ,Huang Shaowei ,Li Bingtao ,Luo Fuhe. STUDY ON NUMERICAL TAXONOMY OF ULMACEAE PLANTS FROM SOUTH CHINA[J]. Journal of South China Agricultural University, 1995, (3): 51-55.

Cited By

Cited by

Periodical cited type(7)

1.	李旭，孙文松，刘莹，杨正书. 北五味子有效成分含量与土壤因子的相关性分析. 园艺与种苗. 2024(01): 68-71 .
2.	郭城峰，曲娜，吕丽娟. 防风种子不同部位浸提液对白菜种子发芽及幼苗生长的影响. 种子科技. 2024(09): 39-42 .
3.	马丙如，崔静轩，王雨懿，刘子君，宁凝，王云贺，杨利民，韩忠明. 防风药材质量的多指标综合评价. 浙江农林大学学报. 2024(04): 715-723 .
4.	李安辉，吕泽良，韩忠明，韩梅，杨利民. 不同碱性盐处理对防风种子萌发、幼苗生长及其生理特性的影响. 中药材. 2023(08): 1870-1875 .
5.	郝佳，刘宇航，殷洁，刘晶，田新，韩忠明，王云贺，韩梅，杨利民. 不同磷浓度对土壤理化性质及防风生长和药材品质的影响. 华南农业大学学报. 2022(03): 59-67 . 本站查看
6.	王浩，秦义杰，钟荣荣，马东来，曾燕，王继永. 基于MaxEnt模型的防风潜在种植区预测. 中国现代中药. 2022(04): 606-610 .
7.	岳超，徐普，刘柱，施贝，张文婷，王峰. HPLC法同时测定玉屏风口服液中12种成分及质量一致性评价. 中成药. 2022(11): 3434-3439 .

Other cited types(4)

Get Citation

PDF

XML

Article views (334) PDF downloads (16) Cited by(11)

Turn off MathJax

Article Contents

Abstract

References

Effect of feeding level on feeding ecology of Pelodiscus sinensis cultured in rice field