EMS mutagenesis breeding of <i>Lagerstroemia speciosa</i>

LIANG Jian; ZHANG Baojin; LIU Shihan; SUN Xugao; FANG Shuyi; DENG Xiaomei

doi:10.7671/j.issn.1001-411X.202404028

Journal of South China Agricultural University > 2025 > 46(2): 203-211. > DOI: 10.7671/j.issn.1001-411X.202404028

LIANG Jian, ZHANG Baojin, LIU Shihan, et al. EMS mutagenesis breeding of Lagerstroemia speciosa[J]. Journal of South China Agricultural University, 2025, 46(2): 203-211. DOI: 10.7671/j.issn.1001-411X.202404028

Citation:

PDF (1013 KB)

EMS mutagenesis breeding of Lagerstroemia speciosa

LIANG Jian^{1, 2,},
ZHANG Baojin^{1, 2},
LIU Shihan^{1, 2},
SUN Xugao^{1, 2},
FANG Shuyi¹,
DENG Xiaomei^{1, 2, ,}

1.
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
2.
Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou 510642, China

More Information

Received Date: April 16, 2024
Available Online: October 29, 2024
Published Date: October 28, 2024

Graphical Abstract

Abstract

Abstract

Objective
In order to enrich the breeding methods of Lagerstroemia speciosa, the optimum conditions of ethyl methane sulfonate (EMS) mutagenesis were studied.
Method
The seeds of L. speciosa were used as the material to compare and analyze the effects of different combinations of EMS concentrations and treatment time on seed germination and seedling growth of L. speciosa.
Result
EMS treatment could inhibit the germination of L. speciosa seeds. The EMS concentration and treatment time had significant impacts on the degree of inhibition. All seeds were killed by treating with 20 g·L⁻¹ EMS for 12 h. The starting time of seed germination was delayed and the germination duration became longer with the increase of EMS concentration and treatment time, and the inhibition of hypocotyl and radicle growth was more significant. The relative lethal rates of the 16 g·L⁻¹ EMS treatment for 12 h and the 18 g·L⁻¹ EMS treatment for 10 h were 47.99% and 55.02%, respectively, which were the closest to the lethal concentration 50(LC50), and these treatment combinations could be chosen for EMS mutagenesis of L. speciosa. The growth of L. speciosa seedlings was inhibited by EMS treatment, with the greatest impact observed at 20 g·L⁻¹ EMS treatment for 8 h. The plant height, crown width, ground diameter and leaf area were 8.28 cm, 9.66 cm, 1.70 mm and 29.32 cm² respectively. The leaf shape index was found to be the highest after 20 g·L⁻¹ EMS treatment for 8 h, with a value of 2.44.
Conclusion
In this study, the treatment combinations with relative lethal rates close to the LC50 in EMS mutagenesis of L. speciosa seeds are determined, which could provide a basis for the cultivation of new varieties of this plant.
- Lagerstroemia speciosa,
- EMS mutagenesis,
- Breeding,
- Seed germination,
- Seedling growth

FullText(HTML)

References (35)

References

[1]	ZHANG Q X. Studies on cultivars of crape-myrtles (Lagerstroemia indica) and their uses in urban greening[J]. Journal of Beijing Forestry University, 1991, 4: 57-66.
[2]	POUNDERS C, RINEHART T, SAKHANOKHO H. Evaluation of interspecific hybrids between Lagerstroemia indica and L. speciosa[J]. HortScience, 2007, 42(6): 1317-1322.
[3]	袁娟. 大花紫薇生殖生物学研究[D]. 南宁: 广西大学, 2017: 73.
[4]	中国科学院中国植物志编辑委员会. 中国植物志[M]. 北京: 科学出版社, 1983: 97.
[5]	袁汕, 林嘉蓓, 张恒, 等. 大花紫薇及其新品种‘紫婵’光合特性的比较研究[J]. 热带作物学报, 2023, 44(4): 757-765. doi: 10.3969/j.issn.1000-2561.2023.04.012
[6]	陈卓梅, 沈鸿明, 张冬林, 等. 从美国专利品种看中国紫薇育种发展趋势[J]. 浙江林业科技, 2018, 38(1): 49-61. doi: 10.3969/j.issn.1001-3776.2018.01.009
[7]	陈之琳. 大花紫薇MADS-box家族B类和C类基因克隆及表达模式研究[D]. 北京: 北京林业大学, 2017: 78.
[8]	焦垚. 大花紫薇与紫薇杂交后代重要观赏性状遗传分析[D]. 北京: 北京林业大学, 2017: 96.
[9]	林嘉蓓, 方姝懿, 邓小梅, 等. 大花紫薇新品种‘紫婵’[J]. 园艺学报, 2021, 48(S2): 2987-2988.
[10]	HE L F. Progress of EMS on application in crop breeding[J]. Seed, 2008, 27(2): 47-49.
[11]	LETHIN J, SHAKIL S S M, HASSAN S, et al. Development and characterization of an EMS-mutagenized wheat population and identification of salt-tolerant wheat lines[J]. BMC Plant Biology, 2020, 20(1): 18. doi: 10.1186/s12870-019-2137-8
[12]	KUMAR S P, KUMARI B D R. Impact of ethyl methane sulphonate mutagenesis in Artemisia vulgaris L. under NaCl stress[J]. BioTech, 2021, 10(3): 18. doi: 10.3390/biotech10030018
[13]	LEYSER H, FURNER I J. EMS mutagenesis of Arabidopsis[J]. Methods in Moleculer Biolgy, 2006, 323: 101-103.
[14]	高文杰, 陈敏健, 刘爱琴, 等. 木本植物EMS诱变育种研究进展[J]. 分子植物育种, 2019, 17(20): 6768-6774.
[15]	尚静, 张会, 王圣洁, 等. EMS诱变对茄子种子萌发、幼苗生长和抗氧化系统的影响[J]. 江西农业学报, 2020, 32(2): 33-37.
[16]	翟永琪, 贾利, 马绍鋆, 等. EMS诱变对西瓜种子萌发及农艺性状的影响[J]. 中国蔬菜, 2023(1): 52-59.
[17]	刘世晗, 梁建, 方姝懿, 等. 大花紫薇果实特征和种子萌发特性[J]. 种子, 2022, 41(11): 85-90.
[18]	降云峰, 刘永忠, 李万星, 等. 甲基磺酸乙酯诱变技术在大豆育种上的应用[J]. 园艺与种苗, 2012, 32(6): 12-15.
[19]	HENRY I M, NAGALAKSHMI U, LIEBERMAN M C, et al. Efficient genome-wide detection and cataloging of EMS-induced mutations using exome capture and next-generation sequencing[J]. The Plant Cell, 2014, 26(4): 1382-1397. doi: 10.1105/tpc.113.121590
[20]	王育川, 董艳辉, 温鑫, 等. 藜麦种子EMS诱变条件初探[J]. 种子, 2021, 40(1): 88-94.
[21]	伊风艳, 孙海莲, 王晓娟, 等. EMS诱变对蒙古国黄花苜蓿种子萌发和幼苗生长的影响[J]. 种子, 2018, 37(10): 41-44.
[22]	赵塔, 王华磊, 李丹丹, 等. 党参种子EMS诱变条件分析[J]. 种子, 2021, 40(6): 20-25.
[23]	李颜方, 杜艳伟, 王高鸿, 等. EMS诱变对晋谷21号种子萌发的影响[J]. 种子, 2020, 39(11): 83-87.
[24]	刘丹阳. 利用EMS诱变紫粒小麦创制小麦新种质[D]. 泰安: 山东农业大学, 2022: 75.
[25]	TALEBI A B, TALEBI A B, SHAHROKHIFAR B. Ethyl methane sulphonate (EMS) induced mutagenesis in Malaysian rice (cv. MR219) for lethal dose determination[J]. American Journal of Plant Sciences, 2012, 3(12): 1661-1665.
[26]	JAIN S M. Mutagenesis in crop improvement under the climate change[J]. Romanian Biotechnological Letters, 2010, 15(2): 88-106.
[27]	ADEKOLA O F, OLULEYE F. Induced morphological variations among Capsicum annum ‘Tatase’ mutants[J]. Production Agriculture and Technology, 2012, 8(2): 50-58.
[28]	ALCANTARA T P, BOSLAND P W, SMITH D W. Ethyl methanesulfonate-induced seed mutagenesis of Capsicum annuum[J]. Journal of Heredity, 1996, 87(3): 239-241. doi: 10.1093/oxfordjournals.jhered.a022992
[29]	伍汉斌, 万志兵, 刘继虎. 不同浓度EMS对紫薇幼苗的诱变效应[J]. 凯里学院学报, 2017, 35(3): 88-91. doi: 10.3969/j.issn.1673-9329.2017.03.22
[30]	BIRD R M, NEUFFER M G. Plant breeding reviews[M]. New York: Van Nostrand Reinhold, 1987: 139-180.
[31]	续言, 王景芸, 吴宜静, 等. EMS处理对尾叶紫薇种子萌发的影响及半致死剂量的研究[J]. 种子, 2022, 41(3): 1-5.
[32]	常媚瑕. ⁶⁰Co-γ、EMS及秋水仙素对朝天椒种子诱变效应的影响[D]. 贵阳: 贵州大学, 2021: 104.
[33]	谢玲玲, 周火强, 弭宝彬, 等. EMS诱变技术研究概况及应用进展[J]. 湖南农业科学, 2020(6): 92-95.
[34]	张慧, 阮明菊, 王韦, 等. 辣木甲基磺酸乙酯(EMS)诱变及表型变异的初步研究[J]. 云南农业大学学报(自然科学), 2022, 37(2): 302-310.
[35]	GREENE E A, CODOMO C A, TAYLOR N E, et al. Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis[J]. Genetics, 2003, 164(2): 731-740. doi: 10.1093/genetics/164.2.731

Cited By

Get Citation

PDF

XML

Article views (104) PDF downloads (35)

Turn off MathJax

Article Contents

Abstract

References

EMS mutagenesis breeding of Lagerstroemia speciosa

Abstract

References

Catalog

Export File

Citation

Format

Content