- Chinese Core Journal
- Chinese Science Citation Database (CSCD) Source journal
- Journal of Citation Report of Chinese S&T Journals (Core Edition)
| Citation: |
YANG Tianmu, WANG Yiheng, XIONG Wenguang, et al. A fast method for detecting Salmonella enteritidis based on PCR-CRISPR/Cas12a[J]. Journal of South China Agricultural University, 2025, 46(3): 311-318. DOI: 10.7671/j.issn.1001-411X.202403028
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To realize early detection, prevention and control of Salmonella enteritidis, a detection method was established by combining CRISPR(Clustered regularly interspaced short palindromic repeat) technology with polymerase chain reaction (PCR).
PCR primers were screened based on the conserved sdfI gene sequence. The sensitivity test was conducted using S. enteritidis genomes at different concentrations, while the specificity tests were conducted using the genomes of Escherichia coli, Staphylococcus aureus, Pasteurella multocida, Salmonella choleraesuis and Enterococcus faecalis. Subsequently, the developed method was applied for the detection of S. enteritidis in small intestinal samples.
This method was capable of identifying S. enteritidis in small intestinal samples, with a detection limit of 1.72 × 100 μL−1, showing excellent specificity and no cross-reactivity with E. coli, S. aureus, P. multocida, S. choleraesuis and E. faecalis.
This study develops a compact diagnostic method for the early detection of S. enteritidis, showing excellent sensitivity and specificity. This innovation presents a fresh perspective for the swift identification of S. enteritidis.
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