Identification of pathogen causing leaf spot disease of <i>Parthenocissus tricuspidata</i> and establishment of LAMP rapid detection system

HUANG Chengyu; CHEN Jianxin; ZHENG Xingyue; WU Fengjinglin; MA Huancheng; WU Jianrong

doi:10.7671/j.issn.1001-411X.202401012

Journal of South China Agricultural University > 2025 > 46(1): 89-96. > DOI: 10.7671/j.issn.1001-411X.202401012

HUANG Chengyu, CHEN Jianxin, ZHENG Xingyue, et al. Identification of pathogen causing leaf spot disease of Parthenocissus tricuspidata and establishment of LAMP rapid detection system[J]. Journal of South China Agricultural University, 2025, 46(1): 89-96. DOI: 10.7671/j.issn.1001-411X.202401012

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Identification of pathogen causing leaf spot disease of Parthenocissus tricuspidata and establishment of LAMP rapid detection system

1.
College of Biodiversity Conservation, Southwest Forestry University/Key Laboratory of Forest Disaster Warning and Control in Universities of Yunnan Province, Kunming 650224, China
2.
College of Forestry, Southwest Forestry University/Key Laboratory of Biodiversity Conservation in Southwest China, National Forestry and Grassland Administration, Kunming 650224, China

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Received Date: January 09, 2024
Available Online: March 26, 2024
Published Date: March 19, 2024

Graphical Abstract

Abstract

Abstract

Objective
To identify the pathogens causing leaf spot disease of Parthenocissus tricuspidata in Yunnan Yulong County, and establish a rapid detection method.
Method
The pathogens were isolated by tissue separation method and verified by Koch’s postulate. Through morphological characteristics and multi-gene joint construction of phylogenetic tree, the taxonomic status of pathogenic bacteria was clarified. A loop-mediated isothermal amplification (LAMP) assay was established by designing specific primers based on the Alt a1 gene sequence of the pathogen.
Result
The pathogen causing P. tricuspidata leaf spot was identified as Alternaria alternata. The established LAMP reaction system could specifically and effectively detect A. alternata. The optimal reaction temperature of the system was 65 ℃, the optimal reaction time was 50 min, and the minimum detection sensitivity was 1 pg/μL. The system was used to detect A. alternata in the leaves of the P. tricuspidata artificially inoculated with pathogens at different time, and the detection time was 12 h or more.
Conclusion
This study provides a theoretical basis for early detection, scientific prevention and control of the leaf spot of P. tricuspidata.
- Parthenocissus tricuspidata,
- Leaf spot,
- Alternaria alternata,
- Pathogen identification,
- LAMP system

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References (24)

References

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Identification of pathogen causing leaf spot disease of Parthenocissus tricuspidata and establishment of LAMP rapid detection system

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