Screening and identification of rice salt-tolerant mutants at germination stage induced by space mutagenesis and heavy ion radiation

ZHAO Zhe; LUO Zengtong; PU Na; ZHAO Ersheng; XIANG Song; ZHANG Bo; HE Linrui; ZHOU Chuqiao; GUO Tao; XIAO Wuming

doi:10.7671/j.issn.1001-411X.202310034

Journal of South China Agricultural University > 2025 > 46(1): 25-33. > DOI: 10.7671/j.issn.1001-411X.202310034

ZHAO Zhe, LUO Zengtong, PU Na, et al. Screening and identification of rice salt-tolerant mutants at germination stage induced by space mutagenesis and heavy ion radiation[J]. Journal of South China Agricultural University, 2025, 46(1): 25-33. DOI: 10.7671/j.issn.1001-411X.202310034

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Screening and identification of rice salt-tolerant mutants at germination stage induced by space mutagenesis and heavy ion radiation

1.
National Engineering Research Center of Plant Space Breeding Technology, South China Agricultural University, Guangzhou 510642, China
2.
Guangdong Tianhong Seed Industry Co., Ltd., Zhanjiang 524006, China

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Received Date: October 26, 2023
Available Online: August 19, 2024
Published Date: August 25, 2024

Graphical Abstract

Abstract

Abstract

Objective
To screen salt-tolerant mutants during germination, provide excellent germplasm resources for creating and breeding new salt-tolerant rice varieties.
Method
The dried seeds of the high quality indica rice variety ‘Hangjuxiangsimiao’, were treated with spatial mutagenesis and heavy ion mutagenesis, and the mutagenised progeny were subjected to targeted screening for salt-tolerant mutants. Wild type (WT) ‘Hangjuxiangsimiao’ seeds were treated with different concentrations of NaCl solution to explore the appropriate concentration for screening salt-tolerant mutants at the germination stage, and then 5 205 M₂ mutant materials were screened using germination rate as an index and verified in the M₃ generation. WT and mutants were measured and analysed for salt-tolerance-related physiological indices and agronomic traits. 48 SSR markers were used to assess the genetic similarity between the mutant progeny and WT.
Result
The appropriate NaCl concentration for screening salt-tolerant mutants at the germination stage was 340 mmol/L, and 12 salt-tolerant mutants were screened at the germination stage. The results of physiological indices showed that the SOD, POD, CAT and APX activities of two representative mutants 9-8 and 22-7 were significantly higher than those of WT, and the MDA, H₂O₂ and O²⁻ contents were significantly lower, the salt-tolerant mutants exhibited stronger antioxidant capacity. The results of SSR marker detection showed that there were no SSR differentiation locus between WT seedlings and mutants of 19-49, 20-15, 20-49, 20-62, 15-6, 16-65, 22-7, 20-19 and 20-38, and one differentiation SSR locus between them and mutants of 9-8, 19-18, 42-113.
Conclusion
Twelve salt-tolerant mutants were screened at the germination stage, which were highly consistent with the genetic background of wild-type ‘Hangjuxiangsimiao’. It has certain theoretical and practical significance for breeding new salt-tolerant rice varieties and studing the genetic mechanism of salt tolerance.
- Rice,
- Space mutation,
- Heavy ion radiation,
- SSR marker,
- Mutant screening,
- Germination stage,
- Salt tolerance

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References (37)

References

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Screening and identification of rice salt-tolerant mutants at germination stage induced by space mutagenesis and heavy ion radiation