- Chinese Core Journal
- Chinese Science Citation Database (CSCD) Source journal
- Journal of Citation Report of Chinese S&T Journals (Core Edition)
| Citation: |
LIN Qiupeng, ZHU Xiuli, MA Linsha, et al. Recent advances in prime editing system[J]. Journal of South China Agricultural University, 2024, 45(2): 159-171. DOI: 10.7671/j.issn.1001-411X.202309002
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LIN Qiupeng: 林秋鹏，华南农业大学首聘教授，博士生导师，研究方向为作物基因组编辑技术开发及应用，主要围绕开发高效、安全的精准编辑体系并将该技术应用于农业育种及医疗领域等开展工作。在国际上率先建立了适用于植物的引导编辑技术体系，并开发了多套提升该系统效率的全新方法，此外还开发了一系列基因组编辑新策略并应用于植物基因功能研究或分子育种；相关工作已申请4项PCT国际专利。获博士后创新人才支持计划(博新计划)及中国博士后科学基金一等资助，获中国科学院优秀博士毕业论文。近年来以第一作者(含共同)在《Cell》《Nature Biotechnology》《Nature Protocols》《Molecular Cell》等国际权威杂志发表SCI论文10篇，累计影响因子超过350
Prime editing (PE) system is a newly developed and greatly revolutionized genome editing technology. The system is based on prime editors, which are composed of two components: A fusion protein of nCas9 (H840A) and reverse transcriptase (RT), and a pegRNA which contains a PBS (Primer binding site) sequence and an RT template (RTT) sequence. The PE system can realize all 12 types of base substitutions and small fragment DNA additions and deletions without double-strand breaks, which is a new paradigm for precision editing. In less than 4 years since its development in 2019, the PE system, as a universal technology platform, has been widely used in various fields such as healthcare and agriculture, generating a large number of excellent application cases such as new germplasm resources and gene therapy drugs. PE, as the most flexible and promising new means of precision genome editing, still suffers from low efficiency, insufficient ability to manipulate large fragments, complex design of system components (such as pegRNAs), incomplete evaluation of safety, and still requires in-depth research. This paper described in detail the technical principles and constraints of PE systems, comprehensively summarized the optimization strategies of PE systems since their development, and the current status of PE applications on animal and plant systems and medical fields. It also gave an outlook on the development prospects of PE.
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