Effects of <i>Fusarium oxysporum</i> on the biological characteristics and expression transcriptome of <i>Caenorhabditis elegans</i>

YI Xingying; XIAO Yue; ZHANG Donghua; LIU Li; YAN Xiaohui; WU Jianrong

doi:10.7671/j.issn.1001-411X.202308007

Journal of South China Agricultural University > 2024 > 45(3): 381-389. > DOI: 10.7671/j.issn.1001-411X.202308007

YI Xingying, XIAO Yue, ZHANG Donghua, et al. Effects of Fusarium oxysporum on the biological characteristics and expression transcriptome of Caenorhabditis elegans[J]. Journal of South China Agricultural University, 2024, 45(3): 381-389. DOI: 10.7671/j.issn.1001-411X.202308007

Citation:

PDF (2016 KB)

Effects of Fusarium oxysporum on the biological characteristics and expression transcriptome of Caenorhabditis elegans

1.
College of Biodiversity Conservation, Southwest Forestry University/Key Laboratory of Forest Disaster Warning and Control in Universities of Yunnan, Kunming 650224, China
2.
College of Forestry, Southwest Forestry University/Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, Kunming 650224, China

More Information

Received Date: August 11, 2023
Available Online: February 25, 2024
Published Date: February 26, 2024

Graphical Abstract

Abstract

Abstract

Objective
To investigate how Fusarium oxysporum affects the fundamental biological traits of nematodes (Caenorhabditis elegans), and identify the critical mechanism of nematode responding to F. oxysporum infection.
Method
The active and inactive spores were cocultured with C. elegans to determine the biological characteristics of C. elegans, such as lifespan, body length, motility/reproductive ability and pharyngeal pumping rate. The whole genome sequencing was performed to find the key pathways and biological functions of nematode responding to F. oxysporum infection by GO and KEGG analysis.
Result
The coculture with active spores shortened the longevity of nematodes, significantly decreased the body size and reduced the reproductive ability of nematodes. However, the coculture did not obviously affect the pharyngeal pumping rate and motility ability of nematodes. GO analysis showed that the differentially expressed genes were enriched in biological functions such as innate immune response and oxidoreductase activity. KEGG analysis showed that the differentially expressed genes were enriched in cytochrome P450 metabolizing drug, cytochrome P450 metabolizing xenobiotics, fatty acid metabolism and other pathways.
Conclusion
F. oxysporum has adverse effects on the basic life activities of C. elegans, which provides insight into the natural immune mechanism of nematodes in response to F. oxysporum and the similarity with plant immunity, so as to find plant-related disease resistance genes.
- Fusarium oxysporum,
- Caenorhabditis elegans,
- Biological characteristic,
- Natural immunity,
- Transcriptome,
- Pathogenesis

FullText(HTML)

References (24)

References

[1]	POULIN R, KEENEY D B. Host specificity under molecular and experimental scrutiny[J]. Trends in Parasitology, 2008, 24(1): 24-28. doi: 10.1016/j.pt.2007.10.002
[2]	SCHULZE-LEFERT P, PANSTRUGA R. A molecular evolutionary concept connecting nonhost resistance, pathogen host range, and pathogen speciation[J]. Trends in Plant Science, 2011, 16(3): 117-125. doi: 10.1016/j.tplants.2011.01.001
[3]	THINES M. An evolutionary framework for host shifts - jumping ships for survival[J]. New Phytologist, 2019, 224(2): 605-617. doi: 10.1111/nph.16092
[4]	JIANPING X. Assessing global fungal threats to humans[J]. mLife, 2022, 1(3): 223-240. doi: 10.1002/mlf2.12036
[5]	FIGTREE M, WEEKS K, CHAN L, et al. Colletotrichum gloeosporioides sensu lato causing deep soft tissue mycosis following a penetrating injury[J]. Medical Mycology Case Reports, 2013, 2: 40-43. doi: 10.1016/j.mmcr.2013.01.003
[6]	杜宾. 镰刀菌的分类和研究现状[J]. 太原学院学报(自然科学版), 2017, 35(4): 68-70.
[7]	叶旭红, 林先贵, 王一明. 尖孢镰刀菌致病相关因子及其分子生物学研究进展[J]. 应用与环境生物学报, 2011, 17(5): 759-762.
[8]	NÜRNBERGER T, BRUNNER F, KEMMERLING B, et al. Innate immunity in plants and animals: Striking similarities and obvious differences[J]. Immunological Reviews, 2004, 198(1): 249-266. doi: 10.1111/j.0105-2896.2004.0119.x
[9]	DARDICK C, RONALD P. Plant and animal pathogen recognition receptors signal through non-RD kinases[J]. PLoS Pathogens, 2006, 2(1): e2. doi: 10.1371/journal.ppat.0020002.
[10]	AUSUBEL F M. Are innate immune signaling pathways in plants and animals conserved?[J]. Nature Immunology, 2005, 6(10): 973-979. doi: 10.1038/ni1253
[11]	郭军英. 关于血球计数板的使用及注意事项[J]. 教学仪器与实验, 2009, 25(4): 26-28.
[12]	樊金华, 薛皎亮, 谢映平, 等. 布氏白僵菌液体培养条件的优化研究[J]. 山西大学学报(自然科学版), 2013, 36(2): 271-274.
[13]	STIERNAGLE T. Maintenance of C. elegans[J]. WormBook, 2006: 1-11. doi: 10.1895/wormbook.1.101.1
[14]	台莲梅, 许艳丽, 高凤昌. 尖孢镰刀菌毒素的初步研究[J]. 黑龙江八一农垦大学学报, 2004, 16(4): 9-12.
[15]	周鸿媛, 唐莉莉, 路勇, 等. 脱氧雪腐镰刀菌烯醇、黄曲霉毒素B1和玉米赤霉烯酮对秀丽隐杆线虫的联合毒性研究[J]. 生态毒理学报, 2018, 13(3): 112-121.
[16]	迟东泽, 何源, 刘芳芳, 等. 鹿鞭醇提物对秀丽隐杆线虫衰老的影响[J]. 食品工业科技, 2021, 42(10): 327-335.
[17]	YANG J, WAN Q L, MU Q Z, et al. The lifespan-promoting effect of otophylloside B in Caenorhabditis elegans[J]. Natural Products and Bioprospecting, 2015, 5(4): 177-183. doi: 10.1007/s13659-015-0064-4
[18]	PUKKILA-WORLEY R, AUSUBEL F M, MYLONAKIS E. Candida albicans infection of Caenorhabditis elegans induces antifungal immune defenses [J]. PLoS Pathogens, 2011, 7(6): e1002074. doi: 10.1371/journal.ppat.1002074.
[19]	肖月, 易杏盈, 张东华, 等. 胶孢炭疽菌侵染秀丽隐杆线虫的特性研究[J]. 生物学杂志, 2023, 40(5): 24-28.
[20]	郭倩楠, 周正富, 徐玉泉, 等. 圆锥掘氏梅里霉侵染秀丽隐杆线虫周期的扫描电镜观察[J]. 中国农业科技导报, 2015, 17(2): 81-86.
[21]	HUANG X, LI D, XI L, et al. Caenorhabditis elegans: A simple nematode infection model for Penicillium marneffei[J]. PLoS One, 2014, 9(9): e108764. doi: 10.1371/journal.pone.0108764.
[22]	OKOLI I, BIGNELL E M. Caenorhabditis elegans: Aspergillus fumigatus (nematode-mould) model for study of fungal pathogenesis[J]. British Microbiology Research Journal, 2015, 7(2): 93-99. doi: 10.9734/BMRJ/2015/15838
[23]	ARNONI M V, PAULA C R, AULER M E, et al. Infections caused by Fusarium species in pediatric cancer patients and review of published literature[J]. Mycopathologia, 2018, 183(6): 941-949. doi: 10.1007/s11046-018-0257-6
[24]	郭亦晨. 霉变槟榔中霉菌分离、鉴定及其对秀丽隐杆线虫的影响[D]. 长沙: 湖南农业大学, 2020.

Cited By

Cited by

Periodical cited type(2)

1.	张兴楠，罗龙辉，黄裕鑫，刘吉平. 桑褐斑病病原菌的分离鉴定及高通量测序分析. 蚕业科学. 2024(01): 9-16 .
2.	胡昌雄，杨伟克，刘增虎，李涛，李琼艳，范永慧，董占鹏. 桑蓟马种群动态及异色瓢虫对其的捕食功能反应. 蚕业科学. 2023(03): 225-233 .

Other cited types(0)

Get Citation

PDF

XML

Article views (218) PDF downloads (42) Cited by(2)

Turn off MathJax

Article Contents

Abstract

References

Effects of Fusarium oxysporum on the biological characteristics and expression transcriptome of Caenorhabditis elegans

Abstract

References

Cited by

Periodical cited type(2)

Other cited types(0)

Catalog

Export File

Citation

Format

Content