Isolation and identification of endophytic bacteria from banana fusarium wilt resistant strains and their inhibitory and growth-promoting effects

LI Jin; TAN Dedong; QIU Jiping; YANG Changsheng; XU Gang; TAN Zhiyuan

doi:10.7671/j.issn.1001-411X.202308003

Journal of South China Agricultural University > 2024 > 45(2): 256-265. > DOI: 10.7671/j.issn.1001-411X.202308003

LI Jin, TAN Dedong, QIU Jiping, et al. Isolation and identification of endophytic bacteria from banana fusarium wilt resistant strains and their inhibitory and growth-promoting effects[J]. Journal of South China Agricultural University, 2024, 45(2): 256-265. DOI: 10.7671/j.issn.1001-411X.202308003

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Isolation and identification of endophytic bacteria from banana fusarium wilt resistant strains and their inhibitory and growth-promoting effects

1.
College of Agriculture, South China Agricultural University, Guangzhou 510642, China
2.
School of Resources Environment and Safety Engineering, University of South China, Hengyang 421001, China

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Received Date: August 03, 2023
Available Online: December 13, 2023
Published Date: November 27, 2023

Abstract

Abstract

Objective
To screen and identify endophytic bacteria that can inhibit Fusarium oxysporum f. sp. cubense (Foc) isolated from roots, bulbs and pseudostems of wilt-resistant strains of Williams banana, and study their inhibitory and growth-promoting effects.
Method
Through 16S rRNA gene sequence and physiological and biochemical tests, species identification and phylogenetic analysis of endophytic bacteria in banana fusarium wilt resistant strains were carried out. The plate confrontation test was carried out to detect the antibacterial ability of strain and explore the types of antibacterial related active substances secreted by antagonist. The biocontrol and growth-promoting functions of endophytic bacteria in banana resistant strains to wilt disease were analyzed by pot experiment. The endophytes with good biocontrol and growth promoting effects were selected and verified in field.
Result
Thirteen representative strains were isolated, among which, there were 8 strains with antibacterial activity against Foc 1 and 7 strains with antibacterial activity against Foc 4. Three biocontrol strains with high inhibitory activity and growth promotion ability were selected by the comprehensive results of biocontrol and growth promoting effects in pot and field experiments, including Bacillus velezensis XG1028, Klebsiella mutans XQJ0301 and Bacillus siamensis XG0103.
Conclusion
Three screened strains can enrich the germplasm resources of microorganisms resistant to banana wilt disease, and provide a reference for finding the new safe and environmentally friendly biological control agents controlling banana wilt disease.
- Banana wilt,
- Antagonistic bacteria,
- Antagonistic factor,
- Biocontrol function,
- Growth promoting effect

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References (38)

References

[1]	PEGG K G, COATES L M, O'NEILL W T, et al. The epidemiology of Fusarium wilt of banana[J]. Frontiers in Plant Science, 2019, 10(1): 1395.
[2]	GARCIA R O, RIVERA-VARGAS L I, PLOETZ R, et al. Characterization of Fusarium spp. isolates recovered from bananas (Musa spp. ) affected by Fusarium wilt in Puerto Rico[J]. European Journal Plant Pathology, 2018, 152(3): 599-611. doi: 10.1007/s10658-018-1503-y
[3]	孙雪丽, 郝向阳, 王天池, 等. 香蕉枯萎病防控和抗病育种研究进展[J]. 果树学报, 2018, 35(7): 870-879.
[4]	王伟英, 邹晖, 林江波, 等. 香蕉抗枯萎病育种研究进展[J]. 东南园艺, 2019, 7(4): 56-60.
[5]	CHOW Y Y, RAHMAN S, TING Y S A. Evaluating the host defense responses in oil palm to complex biocontrol endophyte-pathogen-host plant interaction via Fluidigm^® real-time polymerase chain reaction (RT-PCR)[J]. Biological Control, 2019, 129(2): 148-157.
[6]	周登博, 井涛, 张锡炎, 等. 香蕉枯萎病拮抗菌筛选及其抑菌活性[J]. 植物保护学报, 2016, 43(6): 913-921.
[7]	甘林, 杜宜新, 郑加协, 等. 抗病品种在香蕉枯萎病绿色防控上的应用[J]. 热带作物学报, 2016, 37(10): 1945-1948.
[8]	PAPIK J, FOLKMANOVA M, POLIVKOVA-MAJOROVA M, et al. The invisible life inside plants: Deciphering the riddles of endophytic bacterial diversity[J]. Biotechnology Advances, 2020, 44(7): 107614.
[9]	DINI-ANDREOTE F. Endophytes: The second layer of plant defense[J]. Trends in Plant Science, 2020, 25(4): 319-322. doi: 10.1016/j.tplants.2020.01.007
[10]	SANTOYO G, MORENO-HAGELSIEB G, OROZCO-MOSQUEDA M C, et al. Plant growth-promoting bacterial endophytes[J]. Microbiological Research, 2016, 183(2): 92-99.
[11]	罗琼, 叶小莉, 郑冰雅, 等. 植物内生甲基营养型芽孢杆菌防治番茄枯萎病[J]. 泉州师范学院学报, 2017, 35(6): 17-20.
[12]	石玉莹, 宋海慧, 苗爽, 等. 番茄灰霉病和叶霉病拮抗细菌WXCDD51的筛选鉴定及其生防促生作用[J]. 园艺学报, 2017, 44(10): 1925-1936.
[13]	刘元元, 庞学兵, 李国, 等. 棉花黄萎病生防菌的筛选及挥发性抑菌物质检测[J]. 西北农业学报, 2019, 28(5): 820-829.
[14]	LIU Y, TENG K, WANG T, et al. Antimicrobial Bacillus velezensis HC6: Production of three kinds of lipopeptides and biocontrol potential in maize[J]. Journal of Applied Microbiology, 2020, 128(1): 242-254. doi: 10.1111/jam.14459
[15]	XIANG D, YANG X, LIU B, et al. Bio-priming of banana tissue culture plantlets with endophytic Bacillus velezensis EB1 to improve Fusarium wilt resistance[J]. Frontiers in Microbiology, 2023, 14(1): 1146331.
[16]	FAN H, LI S, ZENG L, et al. Biological control of Fusarium oxysporum f. sp. cubense tropical Race 4 using natively isolated Bacillus spp. YN0904 and YN1419[J]. Journal of Fungi, 2021, 7(10): 795.
[17]	周维, 田丹丹, 李朝生, 等. 香蕉内生细菌G9R-3分离鉴定及其拮抗香蕉枯萎病菌活性评价[J]. 西南农业学报, 2020, 33(7): 1493-1498.
[18]	REDBURN A C, PATEL B K. Phylogenetic analysis of Desulfotomaculum thermobenzoicum using polymerase chain reaction-amplified 16S rRNA-specific DNA[J]. FEMS Microbiology Letters, 1993, 113(1): 81-86. doi: 10.1111/j.1574-6968.1993.tb06492.x
[19]	SCHWYN B, NEILANDS J B. Universal chemical assay for the detection and determination of siderophores[J]. Analytical Biochemistry, 1987, 160(1): 47-56. doi: 10.1016/0003-2697(87)90612-9
[20]	范晓静, 杨瑞先, 邱思鑫, 等. 内生芽孢杆菌 BS2的β−1, 4−内切葡聚糖酶基因与定殖相关性[J]. 中国农业科学, 2014, 47(2): 262-272.
[21]	陈秀兰, 张玉忠, 高培基, 等. 渤海湾浅表海水中产低温蛋白酶适冷菌的筛选[J]. 海洋科学, 2000, 24(9): 42-45.
[22]	黄志强, 林白雪, 谢联辉. 产碱性蛋白酶海洋细菌的筛选与鉴定[J]. 福建农林大学学报(自然科学版), 2006, 35(4): 416-420.
[23]	THOMAS L, RAM H, SINGH V P. Inducible cellulase production from an organic solvent tolerant Bacillus sp. SV1 and evolutionary divergence of endoglucanase in different species of the genus Bacillus[J]. Brazilian Journal of Microbiology, 2018, 49(2): 429-442. doi: 10.1016/j.bjm.2017.05.010
[24]	CASTRO G R, STETTLER A O, FERRERO M A. et al. Selection of an extracellular esterase-producing microorganism[J]. Journal of Industrial Microbiology, 1992, 10(3/4): 165-168. doi: 10.1007/BF01569761
[25]	BANO N, MUSARRAT J. Characterization of a new Pseudomonas aeruginosa strain NJ-15 as a potential biocontrol agent[J]. Current Microbiology, 2003, 46(5): 324-328. doi: 10.1007/s00284-002-3857-8
[26]	何齐庄, 金迪, 彭清静. 一株钾长石分解菌的分离·鉴定及系统发育[J]. 安徽农业科学, 2010, 38(1): 23-25.
[27]	狄义宁, 刘鲁峰, 谢林艳, 等. 一株甘蔗内生菌鉴定及其溶磷能力的研究[J]. 作物杂志, 2018(6): 68-75.
[28]	方中达. 植病研究方法[M]. 3版. 北京: 中国农业出版社, 1998.
[29]	林玲, 乔勇升, 顾本康, 等. 植物内生细菌及其生物防治植物病害的研究进展[J]. 江苏农业学报, 2008, 24(6): 969-974.
[30]	PRIYANKA, AGRAWAL T, KOTASTHANE A S, et al. Crop specific plant growth promoting effects of ACCd enzyme and siderophore producing and cynogenic fluorescent Pseudomonas[J]. 3 Biotech, 2017, 7: 27. doi: 10.1007/s13205-017-0602-3
[31]	WOJTKOWIAK A, WITEK K, HENNIG J, et al. Structures of an active-site mutant of a plant 1, 3-β-glucanase in complex with oligosaccharide products of hydrolysis[J]. Acta Crystallographica Section D: Biological Crystallography, 2013, 69(1): 52-62. doi: 10.1107/S0907444912042175
[32]	柴庆凯, 张斌, 常若葵, 等. 解淀粉芽孢杆菌 LJ02 对黄瓜抗灰霉病菌的生防效果及其诱导抗性机理的初步研究[J]. 植物病理学报, 2019, 49(6): 828-835.
[33]	宁爽. 内生假单胞菌BTa14、Bar25促生抗病作用及机理的研究[D]. 烟台: 烟台大学, 2019.
[34]	WOO S M, KIM S D. Confirmation of non-siderophore antifugal substance and cellulase from Bacillus lichemiformis Kll containing antagonistic ability and plant growth promoting activity[J]. Journal of Life Science, 2007, 17(7): 983-989. doi: 10.5352/JLS.2007.17.7.983
[35]	RAMARATHNAM R, SHEN B, YU C, et al. Molecular and biochemical detection of fengycin-and bacillomycin D-producing Bacillus spp., antagonistic to fungal pathogens of canola and wheat[J]. Canadian Journal of Microbiology, 2007, 53(7): 901-911. doi: 10.1139/W07-049
[36]	SUMPAVAPOL P, TONGYONK L, TANASUPAWAT S, et al. Bacillus siamensis sp. nov., isolated from salted crab (poo-khem) in Thailand[J]. International Journal of Systematic and Evolutionary Microbiology, 2010, 60(10): 2364-2370. doi: 10.1099/ijs.0.018879-0
[37]	HUANG Y H, ZHANG X R, XU H, et al. Isolation of lipopeptide antibiotics from Bacillus siamensis: A potential biocontrol agent for Fusarium graminearum[J]. Canadian Journal of Microbiology, 2022, 68(6): 403-411. doi: 10.1139/cjm-2021-0312
[38]	朱亚珠, 夏率博, 陈琳, 等. 一株贝莱斯芽孢杆菌的生长特性及抑菌活性研究[J]. 食品科学技术学报, 2022, 40(1): 85-92.

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Corresponding author: TAN Zhiyuan, zytan@scau.edu.cn

1.
College of Agriculture, South China Agricultural University, Guangzhou 510642, China

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Isolation and identification of endophytic bacteria from banana fusarium wilt resistant strains and their inhibitory and growth-promoting effects