Isolation and identification of endophytic bacteria from banana fusarium wilt resistant strains and their inhibitory and growth-promoting effects

LI Jin; TAN Dedong; QIU Jiping; YANG Changsheng; XU Gang; TAN Zhiyuan

doi:10.7671/j.issn.1001-411X.202308003

Journal of South China Agricultural University > 2024 > 45(2): 256-265. > DOI: 10.7671/j.issn.1001-411X.202308003

LI Jin, TAN Dedong, QIU Jiping, et al. Isolation and identification of endophytic bacteria from banana fusarium wilt resistant strains and their inhibitory and growth-promoting effects[J]. Journal of South China Agricultural University, 2024, 45(2): 256-265. DOI: 10.7671/j.issn.1001-411X.202308003

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Isolation and identification of endophytic bacteria from banana fusarium wilt resistant strains and their inhibitory and growth-promoting effects

1.
College of Agriculture, South China Agricultural University, Guangzhou 510642, China
2.
School of Resources Environment and Safety Engineering, University of South China, Hengyang 421001, China

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Received Date: August 03, 2023
Available Online: December 13, 2023
Published Date: November 27, 2023

Graphical Abstract

Abstract

Abstract

Objective
To screen and identify endophytic bacteria that can inhibit Fusarium oxysporum f. sp. cubense (Foc) isolated from roots, bulbs and pseudostems of wilt-resistant strains of Williams banana, and study their inhibitory and growth-promoting effects.
Method
Through 16S rRNA gene sequence and physiological and biochemical tests, species identification and phylogenetic analysis of endophytic bacteria in banana fusarium wilt resistant strains were carried out. The plate confrontation test was carried out to detect the antibacterial ability of strain and explore the types of antibacterial related active substances secreted by antagonist. The biocontrol and growth-promoting functions of endophytic bacteria in banana resistant strains to wilt disease were analyzed by pot experiment. The endophytes with good biocontrol and growth promoting effects were selected and verified in field.
Result
Thirteen representative strains were isolated, among which, there were 8 strains with antibacterial activity against Foc 1 and 7 strains with antibacterial activity against Foc 4. Three biocontrol strains with high inhibitory activity and growth promotion ability were selected by the comprehensive results of biocontrol and growth promoting effects in pot and field experiments, including Bacillus velezensis XG1028, Klebsiella mutans XQJ0301 and Bacillus siamensis XG0103.
Conclusion
Three screened strains can enrich the germplasm resources of microorganisms resistant to banana wilt disease, and provide a reference for finding the new safe and environmentally friendly biological control agents controlling banana wilt disease.
- Banana wilt,
- Antagonistic bacteria,
- Antagonistic factor,
- Biocontrol function,
- Growth promoting effect

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References (38)

References

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Isolation and identification of endophytic bacteria from banana fusarium wilt resistant strains and their inhibitory and growth-promoting effects

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