- Chinese Core Journal
- Chinese Science Citation Database (CSCD) Source journal
- Journal of Citation Report of Chinese S&T Journals (Core Edition)
| Citation: |
LIANG Wenxuan, WANG Yueping, CHEN Shengjie, et al. Proteomics study of mature rice seeds based on 4D label-free technology[J]. Journal of South China Agricultural University, 2023, 44(5): 742-749. DOI: 10.7671/j.issn.1001-411X.202307015
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To reveal the molecular basis of rice seed maturation at the protein level, and explore the key proteins and metabolic pathways involved in regulating rice seed maturation.
The experiment used mature rice seeds at 30 days after pollination, and conducted mass spectrum identification using 4D label-free quantitative proteomics. Bioinformatics techniques were used to analyze the subcellular localization, domains, GO annotations, and KEGG pathway annotations of the proteins.
A total of 3 484 seed maturation proteins were identified, with most having a relative molecular mass between 10 000 and 100 000. They were mainly distributed in the cytoplasm, nucleus, chloroplasts, mitochondria, and plasma membrane. The protein domain mainly involved the RNA recognition motifs of protein translation and the protein kinase domains of protein phosphorylation modification. GO analysis showed that the proteins of mature seeds were mainly involved in cellular and metabolic processes, mainly related to catalytic activity and binding functions, and were mostly distributed in cell, cellular component, organelle, and cytomembrane. KEGG analysis revealed that proteins were mainly enriched in pathways such as ribosome, protein processing in endoplasmic reticulum, oxidative phosphorylation, and glycolysis. It was speculated that protein translation, processing, and energy metabolism were the main molecular events of rice seed maturation. Furthermore, proteins related to abscisic acid (ABA) signaling and indoleacetic acid (IAA) metabolism were identified, and transcription factors of the NAM, ATAF1/2 and CUC2 (NAC) family were also discovered.
The accumulation of storage substances and energy metabolism are typical characteristics of rice seed maturation, and ABA and IAA signaling pathways are involved in the process of seed maturation.
| [1] |
余泓, 王冰, 陈明江, 等. 水稻分子设计育种发展与展望[J]. 生命科学, 2018, 30(10): 1032-1037.
|
| [2] |
SERA Y, HANAMATA S, SAKAMOTO S, et al. Essential roles of autophagy in metabolic regulation in endosperm development during rice seed maturation[J]. Scientific Reports, 2019, 9: 18544. doi: 10.1038/s41598-019-54361-1.
|
| [3] |
YOU C C, CHEN L, HE H B, et al. iTRAQ-based proteome profile analysis of superior and inferior spikelets at early grain filling stage in japonica rice[J]. BMC Plant Biology, 2017, 17: 100. doi: 10.1186/s12870-017-1050-2.
|
| [4] |
ZHANG H D, CHEN J N, SHAN S L, et al. Proteomic profiling reveals differentially expressed proteins associated with amylose accumulation during rice grain filling[J]. BMC Genomics, 2020, 21: 714. doi: 10.1186/s12864-020-07105-9.
|
| [5] |
TAPPIBAN P, YING Y N, XU F F, et al. Proteomics and post-translational modifications of starch biosynthesis-related proteins in developing seeds of rice[J]. International Journal of Molecular Sciences, 2021, 22(11): 5901. doi: 10.3390/ijms22115901.
|
| [6] |
XU E S, CHEN M M, HE H, et al. Proteomic analysis reveals proteins involved in seed imbibition under salt stress in rice[J]. Frontiers in Plant Science, 2017, 7: 2006. doi: 10.3389/fpls.2016.02006.
|
| [7] |
CHU C, YAN N, DU Y M, et al. iTRAQ-based proteomic analysis reveals the accumulation of bioactive compounds in Chinese wild rice (Zizania latifolia) during germination[J]. Food Chemistry, 2019, 289: 635-644. doi: 10.1016/j.foodchem.2019.03.092
|
| [8] |
XU H B, LIAN L, JIANG M R, et al. Proteomic analysis reveals different involvement of proteins during the maintenance and release of rice seed dormancy[J]. Molecular Breeding, 2019, 39: 60. doi: 10.1007/s11032-019-0963-x.
|
| [9] |
TAN B C, LIM Y S, LAU S. Proteomics in commercial crops: An overview[J]. Journal of Proteomics, 2017, 169: 176-188. doi: 10.1016/j.jprot.2017.05.018
|
| [10] |
AN L, TAO Y, CHEN H, et al. Embryo-endosperm interaction and its agronomic relevance to rice quality[J]. Frontiers in Plant Science, 2020, 11: 587641. doi: 10.3389/fpls.2020.587641.
|
| [11] |
HAKATA M, KURODA M, MIYASHITA T, et al. Suppression of α-amylase genes improves quality of rice grain ripened under high temperature[J]. Plant Biotechnology Journal, 2012, 10(9): 1110-1117. doi: 10.1111/j.1467-7652.2012.00741.x
|
| [12] |
DAMARIS R N, LIN Z Y, YANG P F, et al. The rice alpha-amylase, conserved regulator of seed maturation and germination[J]. International Journal of Molecular Sciences, 2019, 20(2): 450. doi: 10.3390/ijms20020450.
|
| [13] |
PENG L L, SUN S, YANG B, et al. Genome-wide association study reveals that the cupin domain protein OsCDP3.10 regulates seed vigour in rice[J]. Plant Biotechnology Journal, 2022, 20(3): 485-498. doi: 10.1111/pbi.13731
|
| [14] |
宋松泉, 刘军, 唐翠芳, 等. 种子耐脱水性的生理及分子机制研究进展[J]. 中国农业科学, 2022, 55(6): 1047-1063.
|
| [15] |
SMOLIKOVA G, LEONOVA T, VASHURINA N, et al. Desiccation tolerance as the basis of long-term seed viability[J]. International Journal of Molecular Sciences, 2021, 22(1): 101. doi: 10.3390/ijms22010101.
|
| [16] |
ALI F, QANMBER G, LI F G, et al. Updated role of ABA in seed maturation, dormancy, and germination[J]. Journal of Advanced Research, 2022, 35: 199-214. doi: 10.1016/j.jare.2021.03.011
|
| [17] |
宋松泉, 唐翠芳, 雷华平, 等. ABA调控种子发育的研究进展[J/OL]. 广西植物, 2023 [2023-07-29]. https://kns.cnki.net/kcms2/detail/45.1134.Q.20230620.1121.004.html.
|
| [18] |
MATILLA A J. Auxin: hormonal signal required for seed development and dormancy[J]. Plants, 2020, 9(6): 705. doi: 10.3390/plants9060705.
|
| [19] |
LIU X D, ZHANG H, ZHAO Y, et al. Auxin controls seed dormancy through stimulation of abscisic acid signaling by inducing ARF-mediated ABI3 activation in Arabidopsis[J]. Proceedings of the National Academy of Sciences, 2013, 110(38): 15485-15490. doi: 10.1073/pnas.1304651110
|
| [20] |
田淑兰, 王熹. IAA与亚种间杂交稻籽粒发育的关系及烯效唑的调节[J]. 中国水稻科学, 1998, 12(2): 99-104.
|
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