QTL mapping and epistatic effect analysis  of seedling height  based on single segment substitution lines in rice

ZHANG Longting; WU Jing; XIONG Xijuan; DONG Jingfang; ZHANG Shaohong; ZHAO Junliang; LIU Ziqiang; YANG Tifeng

doi:10.7671/j.issn.1001-411X.202306067

Journal of South China Agricultural University > 2023 > 44(6): 881-888. > DOI: 10.7671/j.issn.1001-411X.202306067

ZHANG Longting, WU Jing, XIONG Xijuan, et al. QTL mapping and epistatic effect analysis of seedling height based on single segment substitution lines in rice[J]. Journal of South China Agricultural University, 2023, 44(6): 881-888. DOI: 10.7671/j.issn.1001-411X.202306067

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QTL mapping and epistatic effect analysis of seedling height based on single segment substitution lines in rice

1.
Rice Research Institute/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering Laboratory/Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
2.
College of Agriculture, South China Agricultural University, Guangzhou 510642, China

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Received Date: July 10, 2023
Available Online: November 12, 2023
Published Date: September 13, 2023

Graphical Abstract

Abstract

Abstract

Objective
To find out the stable QTLs controlling rice seedling height, analyze their epistatic effects, and provide QTL and theoretical references for molecular breeding of rice seedling height.
Method
The single segment substitution lines (SSSLs) with IR65598-112-2 as donor and ‘Huajingxian 74’ as receptor were used as materials. The difference of seedling height between SSSL and ‘Huajingxian 74’ was measured, and the QTLs of seedling height were mapped. The QTL interval was narrowed by substitution mapping, and the epistatic effects of two seedling height QTLs were also analyzed.
Result
Two adjacent QTLs (qSH3-1 and qSH3-2) for seedling height were mapped on the long arm of chromosome 3, which were located in the intervals of 32.59−33.08 and 33.16−34.81 Mb, with the lengths of 0.49 and 1.65 Mb, respectively. The additive effects were −0.86 and −1.09 cm, respectively. The phenotypic contribution rate of additive effects were −4.14% and −5.15%, respectively. However, there was no significant difference of seedling height between SSSL harboring these two QTLs and ‘Huajingxian 74’.
Conclusion
Two QTLs for seedling height were identified, and there may be significant epistasis effects between the two QTLs.
- Seedling height,
- Single segment substitution line,
- Substitution mapping,
- QTL mapping,
- Epistatic effect,
- Oryza sativa L.

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References (34)

References

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QTL mapping and epistatic effect analysis of seedling height based on single segment substitution lines in rice

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