Evaluation of heat stress resistance and its molecular machanism of four <i>indica</i> rice cultivars at seedling stage

YE Chanjuan; CHEN Ke; ZHOU Xinqiao; CHEN Dagang; GUO Jie; LIU Juan; CAO Zhe; LIU Chuanguang; CHEN Youding; CHEN Guorong

doi:10.7671/j.issn.1001-411X.202306020

Journal of South China Agricultural University > 2023 > 44(6): 906-914. > DOI: 10.7671/j.issn.1001-411X.202306020

YE Chanjuan, CHEN Ke, ZHOU Xinqiao, et al. Evaluation of heat stress resistance and its molecular machanism of four indica rice cultivars at seedling stage[J]. Journal of South China Agricultural University, 2023, 44(6): 906-914. DOI: 10.7671/j.issn.1001-411X.202306020

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Evaluation of heat stress resistance and its molecular machanism of four indica rice cultivars at seedling stage

1.
Rice Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering Laboratory, Guangzhou 510640, China
2.
College of Life Sciences, South China Agricultural University, Guangzhou 510642, China

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Received Date: June 24, 2023
Available Online: November 12, 2023
Published Date: September 10, 2023

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Abstract

Abstract

Objective
To identify the variation in heat stress resistance among seedlings of four different indica rice cultivars grown in South China, and provide theoretical reference and technical support for the breeding and promotion of rice varieties.
Method
We performed whole-genome re-sequencing, phenotype identification, and transcriptional level analysis to comprehensively evaluate the heat stress resistance for seedlings of high-yield conventional rice cultivar ‘Nan Xiu Mei Zhan’ (NXMZ), hybrid rice restorer cultivar ‘R5518’, and aromatic rice cultivars ‘Jiu Li Xiang’ (JLX) and ‘Nan Jing Xiang Zhan’ (NJXZ) in South China.
Result
The ‘NJXZ’ was sensitive to heat stress. The ‘R5518’ showed medium resistance to heat stress. The resistances to heat stress of ‘JLX’ and ‘NXMZ’at seedling stage were relatively high in comparison to other two rice cultivars. We compared the haplotypes of heat resistance related genes, the relative expression levels and phenotypes, and found that many SNPs appeared in OsTT1 from‘JLX’, while the haplotypes of four cultivars with other heat-related QTLs remained relatively consistent, suggesting that the OsTT1 might contribute to partial heat stress resistance in ‘JLX’. The gene expression patterns in OsHSF7, OsHSP71.1 and OsHTS1 were consistent with the evaluation in heat stress resistance of rice cultivars, indicating that these three genes might associate with regulation in heat stress resistance of four indica rice cultivars.
Conclusion
The gene expression difference, gene shift and transcript error in certain genes result in variations in heat stress resistance of different indica rice cultivars at seedling stage. These results can provide new ideas for genome-wide selection for heat tolerance breeding in rice.
- Rice cultivar,
- Heat stress resistance,
- Seedling stage,
- Whole genome re-sequence,
- Molecular mechanism,
- Genomic variation

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References

[1]	ZHAO C, LIU B, PIAO S, et al. Temperature increase reduces global yields of major crops in four independent estimates[J]. Proceedings of the National Academy of Sciences of the United States of America, 2017, 114(35): 9326-9331.
[2]	陈祖龙, 郑珍珍, 王慧, 等. 水稻耐热育种研究及其展望[J]. 滁州学院学报, 2023, 25(2): 23-28.
[3]	LIU H, ZENG B, ZHAO J, et al. Genetic research progress: Heat tolerance in rice[J]. International Journal of Molecular Sciences, 2023, 24(8): 7140.
[4]	NUBANKOH P, WANCHANA S, SAENSUK C, et al. QTL-seq reveals genomic regions associated with spikelet fertility in response to a high temperature in rice (Oryza sativa L.)[J]. Plant Cell Reports, 2020, 39(1): 149-162. doi: 10.1007/s00299-019-02477-z
[5]	YE C, TENORIO F A, ARGAYOSO M A, et al. Identifying and confirming quantitative trait loci associated with heat tolerance at flowering stage in different rice populations[J]. BMC Genetics, 2015, 16: 41.
[6]	ZHU S, HUANG R, WAI H P, et al. Mapping quantitative trait loci for heat tolerance at the booting stage using chromosomal segment substitution lines in rice[J]. Physiology and Molecular Biology of Plants, 2017, 23(4): 817-825. doi: 10.1007/s12298-017-0465-4
[7]	王丰, 程方民, 刘奕, 等. 不同温度下灌浆期水稻籽粒内源激素含量的动态变化[J]. 作物学报, 2006, 32(1): 25-29.
[8]	LI X M, CHAO D Y, WU Y, et al. Natural alleles of a proteasome alpha2 subunit gene contribute to thermotolerance and adaptation of African rice[J]. Nature Genetics, 2015, 47(7): 827-833. doi: 10.1038/ng.3305
[9]	KAN Y, MU X R, ZHANG H, et al. TT2 controls rice thermotolerance through SCT1-dependent alteration of wax biosynthesis[J]. Nature Plants, 2022, 8(1): 53-67.
[10]	ZHANG H, ZHOU J F, KAN Y, et al. A genetic module at one locus in rice protects chloroplasts to enhance thermotolerance[J]. Science, 2022, 376(6599): 1293-1300. doi: 10.1126/science.abo5721
[11]	CAO Z B, TANG H W, CAI Y H, et al. Natural variation of HTH5 from wild rice, Oryza rufipogon Griff., is involved in conferring high-temperature tolerance at the heading stage[J]. Plant Biotechnology Journal, 2022, 20(8): 1591-1605.
[12]	WANG D, QIN B X, LI X, et al. Nucleolar DEAD-Box RNA helicase TOGR1 regulates thermotolerant growth as a Pre-rRNA chaperone in rice[J]. PLoS Genetics, 2016, 12(2): e1005844. doi: 10.1371/journal.pgen.1005844
[13]	SHEN H, ZHONG X B, ZHAO F F, et al. Overexpression of receptor-like kinase ERECTA improves thermotolerance in rice and tomato[J]. Nature Biotechnology, 2015, 33(9): 996-1003.
[14]	YANG Y, YU J, QIAN Q, et al. Enhancement of heat and drought stress tolerance in rice by genetic manipulation: A systematic review[J]. Rice, 2022, 15(1): 67. doi: 10.1186/s12284-022-00614-z
[15]	YU H, LIU Y, SUN B, et al. Genetic diversity and breeding signatures for regional Indica rice improvement in Guangdong of Southern China[J]. Rice, 2023, 16(1): 25. doi: 10.1186/s12284-023-00642-3
[16]	WANG J, YANG W, ZHANG S, et al. A pangenome analysis pipeline provides insights into functional gene identification in rice[J]. Genome Biology, 2023, 24(1): 19.
[17]	WEI X, QIU J, YONG K, et al. A quantitative genomics map of rice provides genetic insights and guides breeding[J]. Nature Genetics, 2021, 53(2): 243-253. doi: 10.1038/s41588-020-00769-9
[18]	刘传光, 周新桥, 陈达刚, 等. 广东丝苗米品种南晶香占的选育与栽培技术[J]. 农业与技术, 2022, 42(7): 94-97.
[19]	LETUNIC I, BORK P. Interactive tree of Life (iTOL) v5: An online tool for phylogenetic tree display and annotation[J]. Nucleic Acids Research, 2021, 49(W1): W293-W296. doi: 10.1093/nar/gkab301
[20]	CHEN K, YE C, GUO J, et al. Agrobacterium-mediated transformation efficiency and grain phenotypes in six indica and japonica rice cultivars[J]. Seed Biology, 2023, 2: 4. doi: 10.48130/SeedBio-2023-0004.
[21]	ROBINSON J T, THORVALDSDOTTIR H, TURNER D, et al. Igv. js: An embeddable JavaScript implementation of the Integrative Genomics Viewer (IGV)[J]. Bioinformatics, 2023, 39(1): btac830.
[22]	GOPALAKRISHNAN N, KANG I, MOON B, et al. Effects of low temperature stress on rice (Oryza sativa L. ) plastid ω-3 desaturase gene, OsFAD8 and its functional analysis using T-DNA mutants[J]. Plant Cell, Tissue and Organ Culture, 2009, 98(1): 87-96. doi: 10.1007/s11240-009-9541-y
[23]	MOON J, HAM D, HWANG S, et al. Molecular characterization of a heat inducible rice gene, OsHSP1, and implications for rice thermotolerance[J]. Genes & Genomics, 2014, 36(2): 151-161.
[24]	LIU J G, QIN Q L, ZHANG Z, et al. OsHSF7 gene in rice, Oryza sativa L., encodes a transcription factor that functions as a high temperature receptive and responsive factor[J]. BMB Reports, 2009, 42(1): 16-21. doi: 10.5483/BMBRep.2009.42.1.016

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