Functional analysis of <i>OsRAC3</i> regulating seed traits in rice

LIU Huili; GUO Ziming; CHENG Tairan; ZHANG Zhicheng

doi:10.7671/j.issn.1001-411X.202304033

Journal of South China Agricultural University > 2023 > 44(5): 690-695. > DOI: 10.7671/j.issn.1001-411X.202304033

LIU Huili, GUO Ziming, CHENG Tairan, et al. Functional analysis of OsRAC3 regulating seed traits in rice[J]. Journal of South China Agricultural University, 2023, 44(5): 690-695. DOI: 10.7671/j.issn.1001-411X.202304033

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Functional analysis of OsRAC3 regulating seed traits in rice

College of Life Sciences, South China Agricultural University, Guangzhou 510642, China

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Received Date: April 21, 2023
Available Online: November 12, 2023
Published Date: June 29, 2023

Graphical Abstract

Abstract

Abstract

Objective
RAC/ROPs are a class of plant-specific small G proteins, which are involved in multiple signaling pathways as molecular switches. OsRAC3 regulates rice (Oryza sativa L.) crown root development by interacting with cytokinin signaling components. However whether it affects traits in the aboveground parts of rice remains unclear. This study mainly aims to analyze the effects of OsRAC3 on spike development and yield traits of rice.
Method
The inflorescence tissue of rice OsRAC3promoter::GUS transgenic plants were stained with GUS to analyze the expression pattern of OsRAC3. We also analyzed the main agronomic traits such as number of grains per spike, grain length, grain width and thousand grain weight of two experimental materials, constitutively activated mutant (CA-osrac3) and dominant negative mutant (DN-osrac3).
Result
OsRAC3 was strongly expressed in floral meristems, stamen and pistill. CA-osrac3 had fewer grains but its grain length, grain width and thousand grain weight were significantly higher than those of wild-type. DN-osrac3 had a low number of spike branches and poor fertility, while the grain length, grain width and thousand grain weight were significantly lower than those of wild-type.
Conclusion
OsRAC3 affects the developmental process of rice spikes, and the constitutively activated mutant CA-osrac3 promotes the growth of rice seeds. OsRAC3 has important applications in high-yield breeding and genetic improvement of rice.
- Oryza sativa L.,
- RAC/ROP,
- OsRAC3,
- Spike,
- Grain

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References (45)

References

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