Effects of fasting on intestinal barrier and sensory vagus receptor expression in yellow-feathered broilers

SUI Chengrong; LI Qiqi; PAN Linghui; GUO Yafei; SHI Cuihan; JIANG Qingyan; SHU Gang; ZHU Canjun

doi:10.7671/j.issn.1001-411X.202211048

Journal of South China Agricultural University > 2024 > 45(2): 172-178. > DOI: 10.7671/j.issn.1001-411X.202211048

SUI Chengrong, LI Qiqi, PAN Linghui, et al. Effects of fasting on intestinal barrier and sensory vagus receptor expression in yellow-feathered broilers[J]. Journal of South China Agricultural University, 2024, 45(2): 172-178. DOI: 10.7671/j.issn.1001-411X.202211048

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Effects of fasting on intestinal barrier and sensory vagus receptor expression in yellow-feathered broilers

College of Animal Science, South China Agricultural University, Guangzhou 510642, China

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Received Date: November 01, 2022
Available Online: December 17, 2023
Published Date: December 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
The aim of this study was to investigate the changes of intestinal inflammatory signals and vagal nerve related receptors in yellow-feathered broilers under starvation.
Method
Yellow-feathered broilers were used in the experiment. They were randomly divided into control group and fasting group according to the principle of consistent body weight. The expressions of appetite peptides, intestinal tight junction proteins and inflammatory factors in hypothalamus and receptors related to inflammation and appetite regulation in nodose ganglia (NG) of yellow-feathered broilers were detected by q-PCR. The morphological changes of intestinal tract were observed and statistically analyzed by electron microscopy and HE staining.
Result
Compared with the control group, short-term fasting increased the expressions of hypothalamic orexigenic peptides AgRP (P<0.05) and NPY (P<0.01). Short-term fasting increased the concentration of intestinal H₂O₂, promoted the expressions of small intestinal tight junction protein (P<0.05), decreased the proportion of jejunal injury (P<0.01), and increased the absorption capacity of jejunal villi (P<0.01). Short-term fasting increased the expressions of anti-inflammatory factors IL-4 and IL-10 in jejunal mucosa (P<0.01). In addition, short-term fasting not only promoted the expressions of FFAR2 and NPY2R in NG (P<0.01), but also increased the expression of IL-4R (P<0.01).
Conclusion
Starvation can increase the levels of intestinal anti-inflammatory factors, enhance intestinal barrier function, and promote the expressions of receptors such as vagal anti-inflammatory factors in yellow-feathered broilers.
- Yellow-feathered broiler,
- Food intake,
- Intestinal inflammation,
- Intestinal barrier,
- Nodose ganglion

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References (33)

References

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