Cloning and functional verification of circadian clock gene <i>MtTOC1a</i> in <i>Medicago truncatula</i>

DUAN Tingting; YANG Mingkang; HUANG Ke; HUANG Wei

doi:10.7671/j.issn.1001-411X.202211001

Journal of South China Agricultural University > 2023 > 44(5): 803-809. > DOI: 10.7671/j.issn.1001-411X.202211001

DUAN Tingting, YANG Mingkang, HUANG Ke, et al. Cloning and functional verification of circadian clock gene MtTOC1a in Medicago truncatula[J]. Journal of South China Agricultural University, 2023, 44(5): 803-809. DOI: 10.7671/j.issn.1001-411X.202211001

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Cloning and functional verification of circadian clock gene MtTOC1a in Medicago truncatula

1.
College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
2.
Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms/ Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China

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Received Date: October 31, 2022
Available Online: November 12, 2023
Published Date: August 27, 2023

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Abstract

Abstract

Objective
The goal of this study is to analyze the protein structure of Medicago truncatula MtTOC1a, explore the biological function of MtTOC1a in the circadian clock system, and compare its similarities and differences in function with its ortholog AtTOC1 in Arabidopsis thaliana.
Method
The orthologous genes of TOC1 in Medicago were identified through bioinformatics analysis, the expression vector of MtTOC1a gene was constructed and introduced into Arabidopsis wild-type Col and the corresponding loss-of-function mutant toc1-2 by Agrobacterium mediated method for genetic complementation analysis. Both MtTOC1a and MtTOC1b have conserved functional domains and protein structures. The genetic analysis indicated that during early photomorphogenesis, exogenously transformed MtTOC1a fully restored the hypocotyl elongation phenotype of toc1-2, but had no significant effect on the premature flowering phenotype of toc1-2. In the CAB::LUC reporter lines, MtTOC1a lengthened the period of the short period mutant toc1-2 under continuous light conditions, yet the mutant could not fully recover to the wild-type level.
Conclusion
MtTOC1a and AtTOC1 have similar functions, but their roles in drownstream pathways are still different. The results provide a theoretical basis for further exploring the function of MtTOC1a gene and using MtTOC1a gene to modify the important traits in Medicago.
- Medicago truncatula,
- Arabidopsis thaliana,
- MtTOC1a,
- Circadian clock,
- Circadian rhythm,
- Agronomic trait

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Cloning and functional verification of circadian clock gene MtTOC1a in Medicago truncatula

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