Effects of compound Chinese medicine ultrafine powders on egg production, reproductive hormone, and related gene expression in laying hens

HAN Kunliang; LIN Wenchao; MENG Chengwen; LAN Wei; HU Xin; CUI Yadong; KONG Xiangfeng

doi:10.7671/j.issn.1001-411X.202206047

Journal of South China Agricultural University > 2023 > 44(3): 374-381. > DOI: 10.7671/j.issn.1001-411X.202206047

HAN Kunliang, LIN Wenchao, MENG Chengwen, et al. Effects of compound Chinese medicine ultrafine powders on egg production, reproductive hormone, and related gene expression in laying hens[J]. Journal of South China Agricultural University, 2023, 44(3): 374-381. DOI: 10.7671/j.issn.1001-411X.202206047

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Effects of compound Chinese medicine ultrafine powders on egg production, reproductive hormone, and related gene expression in laying hens

1.
Institute of Subtropical Agriculture, Chinese Academy of Sciences/Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Processes, Changsha 410125, China
2.
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730030, China
3.
School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China

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Corresponding author:
professor, E-mail: nnkxf@isa.ac.cn
Received Date: June 28, 2022
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
This study was aimed to determine the effect of compound Chinese medicine ultrafine powders on egg laying performance, reproductive hormone, and related gene expression in laying hens.

Method
A total of 216 Xinyang black-feather laying hens (307-day-old) were selected and randomly divided into three groups with eight replicates in each group and nine in each replicate. The control group was fed the basal diet, and the experimental groups were fed basal diets supplemented with 0.5% Salvia miltiorrhiza (SM) + 0.25% Ligustrum lucidum (LL) + 0.25% Taraxacum mongolicum (TM)(compound 1), and 0.3% Leonurus japonicus (LJ) + 0.2% SM + 0.25% LL + 0.25% TM (compound 2) ultrafine powders, respectively. The pre-trial period was seven days, and the formal trial period was 120 days. Egg production performance, egg quality, and plasma reproductive hormone level were determined. The expressions of genes related to egg quality were detected by RT-PCR technology.

Result
Compared with the control group, during days 1−30, 31−60, and 1−120 of the trial, the egg laying rates of the compound 1 group increased by 7.56%, 4.00%, and 5.31% (P<0.05), respectively, and during 1−120 days the egg laying rate of the compound 2 group increased by 5.22% (P<0.05). On day 60 after treatment the plasma estradiol level and relative expression of oviductPMCA1 mRNA increased by 21.45% and 77.00% (P<0.05), respectively, in the compound 1 group, and the relative expression of oviductKCNA1 mRNA increased by 70.00% (P<0.05) in the compound 2 group. On day 90, the thickness of the eggshells increased by 12.12% and 9.09% (P<0.05), respectively, in the compound 1 and 2 groups. On day 120, the relative expressions of oviductCA2 mRNA increased by 86.00% and 66.00% (P<0.05), respectively, in the compound 1 and 2 groups, and the relative expressions of oviductCDH6,KCNA1 andSLC26A9 mRNA increased by 99.00%, 86.00% and 99.00% (P<0.05), respectively, in the compound 2 group.

Conclusion
Dietary supplementation with compound Chinese medicine ultrafine powders consisting of LJ, SM, LL, and TM could increase the egg production rate, improve egg quality of laying hen, increase the level of reproductive hormone, and up-regulate mRNA expressions of genes associated with eggshell formation. In addition, compound 1 presents the better effect.
- Chinese medicine ultrafine powders,
- Laying hen,
- Egg production performance,
- Egg quality,
- Reproductive hormone,
- Gene expression

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References (25)

References

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