CHEN Weiguo, KUANG Zhixiang, ZHANG Xiangyu, et al. Effect of initiation codon mutation within tva receptor gene on chicken resistance to infection by avian leukemia virus subgroup A[J]. Journal of South China Agricultural University, 2023, 44(3): 367-373. DOI: 10.7671/j.issn.1001-411X.202206019
    Citation: CHEN Weiguo, KUANG Zhixiang, ZHANG Xiangyu, et al. Effect of initiation codon mutation within tva receptor gene on chicken resistance to infection by avian leukemia virus subgroup A[J]. Journal of South China Agricultural University, 2023, 44(3): 367-373. DOI: 10.7671/j.issn.1001-411X.202206019

    Effect of initiation codon mutation within tva receptor gene on chicken resistance to infection by avian leukemia virus subgroup A

    More Information
    • Received Date: June 14, 2022
    • Available Online: May 17, 2023
    • Objective 

      To explore the effect of initiation codon mutation within tva receptor gene (tva c.3G>A) on resistance of chickens to infection by avian leukemia virus subgroup A (ALV-A).

      Method 

      Sanger sequencing and RT-PCR were used to verify the presence of tva c.3G>A mutation in Chinese yellow-feathered broilers. The effect oftva c.3G>A mutation on infection of chickens by RCASBP(A)-GFP fluorescence reporter virusin vitro was detected using flow cytometry. The effect of tva c.3G>A mutation on infection of chickens by ALV-A was investigated using ALV-A challenge testin vivo. Direct sequencing was used to genotypetva c.3G>A mutation site within Chinese yellow-feathered broiler lines.

      Result 

      The results of Sanger sequencing and RT-PCR identified the mutation from G to A on the third base in the coding region of tva gene of Chinese yellow-feathered broilers, which caused the mutation from ATG to ATA in the initial codon sequence of tva gene. The result of flow cytometry showed that chicken embryo fibroblasts (CEFs) of wild-type tva c.3G/G were susceptible to infection by RCASBP(A)-GFP, while the homozygous mutant tva c.3A/A CEFs were resistant to infection by RCASBP(A)-GFP, indicating that tva c.3G>A mutation led to chicken resistance to infection by RCASBP(A)-EGFPin vitro. The results of ALV-A challenge test in vivo also indicated that tva c.3G>A mutation led to chicken resistance to infection by ALV-A. Genotyping oftva c.3G>A revealed that homozygous resistance genotypetva c.3A/A was present in lines CB01, CB08, CB10 and CB15, with the frequencies of 0.10, 0.15, 0.23 and 0.08, respectively.

      Conclusion 

      The tva c.3G>A mutation causes chicken resistance to infection by ALV-Ain vitro and in vivo, and the tva c.3G>A mutation site can be used as the genetic resistance site of ALV-A.

    • [1]
      李德娟, 崔梦笛, 王学静, 等. 禽白血病病毒及其受体研究进展[J]. 黑龙江畜牧兽医, 2022(9): 34-41.
      [2]
      王鑫, 赵鹏, 崔治中. 我国地方品种鸡分离到的一个禽白血病病毒新亚群的鉴定[J]. 病毒学报, 2012, 28(6): 609-614. doi: 10.13242/j.cnki.bingduxuebao.002329
      [3]
      PAYNE L N, NAIR V. The long view: 40 years of avian leukosis research[J]. Avian Pathology, 2012, 41(1): 11-19. doi: 10.1080/03079457.2011.646237
      [4]
      崔治中. 禽白血病病毒研究的过去、现在和将来[J]. 生命科学, 2012, 24(4): 305-309. doi: 10.13376/j.cbls/2012.04.001
      [5]
      崔治中. 种鸡场禽白血病防控和净化技术方案[J]. 中国家禽, 2015, 37(23): 1-7. doi: 10.16372/j.issn.1004-6364.2015.23.001
      [6]
      俞燕. 地方品种鸡群禽白血病流行病学调查及检测净化技术研究[D]. 扬州: 扬州大学, 2019.
      [7]
      SU Q, LI Y, LI W, et al. Molecular characteristics of avian leukosis viruses isolated from indigenous chicken breeds in China[J]. Poultry Science, 2018, 97(8): 2917-2925. doi: 10.3382/ps/pex367
      [8]
      冯敏, 谭利强, 代曼曼, 等. 种禽场A亚群禽白血病病原学调查及分离株遗传进化分析[J]. 华南农业大学学报, 2014, 35(4): 11-15. doi: 10.7671/j.issn.1001-411X.2014.04.003
      [9]
      钱琨, 朱钰峰, 沈海玉, 等. 地方蛋鸡群A亚群禽白血病病毒的分离与全基因组序列分析[J]. 中国兽医科学, 2011, 41(10): 1005-1010.
      [10]
      杨波, 高玉龙, 高宏雷, 等. 我国东北地区野生鸟类A亚群禽白血病病毒分子流行病学调查及env基因序列分析[J]. 中国预防兽医学报, 2013, 35(3): 245-247. doi: 10.3969/j.issn.1008-0589.2013.03.18
      [11]
      BACON L D, HUNT H D, CHENG H H. A review of the development of chicken lines to resolve genes determining resistance to diseases[J]. Poultry Science, 2000, 79(8): 1082-1093. doi: 10.1093/ps/79.8.1082
      [12]
      JIE H, LIU Y P. Breeding for disease resistance in poultry: Opportunities with challenges[J]. World’s Poultry Science Journal, 2011, 67(4): 687-696. doi: 10.1017/S0043933911000766
      [13]
      CHENG H H, KAISER P, LAMONT S J. Integrated genomic approaches to enhance genetic resistance in chickens[J]. Annual Review of Animal Biosciences, 2013, 1: 239-260. doi: 10.1146/annurev-animal-031412-103701
      [14]
      BATES P, YOUNG J A, VARMUS H E. A receptor for subgroup A Rous sarcoma virus is related to the low density lipoprotein receptor[J]. Cell, 1993, 74(6): 1043-1051. doi: 10.1016/0092-8674(93)90726-7
      [15]
      BARNARD R J, ELLEDER D, YOUNG J A. Avian sarcoma and leukosis virus-receptor interactions: From classical genetics to novel insights into virus-cell membrane fusion[J]. Virology, 2006, 344(1): 25-29. doi: 10.1016/j.virol.2005.09.021
      [16]
      ELLEDER D, MELDER D C, TREJBALOVA K, et al. Two different molecular defects in the Tva receptor gene explain the resistance of two tvar lines of chickens to infection by subgroup A avian sarcoma and leukosis viruses[J]. Journal of Virology, 2004, 78(24): 13489-13500. doi: 10.1128/JVI.78.24.13489-13500.2004
      [17]
      REINISOVA M, PLACHY J, TREJBALOVA K, et al. Intronic deletions that disrupt mRNA splicing of the tva receptor gene result in decreased susceptibility to infection by avian sarcoma and leukosis virus subgroup A[J]. Journal of Virology, 2012, 86(4): 2021-2030. doi: 10.1128/JVI.05771-11
      [18]
      CHEN W, LIU Y, LI H, et al. Intronic deletions of tva receptor gene decrease the susceptibility to infection by avian sarcoma and leukosis virus subgroup A[J]. Scientific Reports, 2015, 5: 9900. doi: 10.1038/srep09900
      [19]
      LIAO C T, CHEN S Y, CHEN W G, et al. Single nucleotide polymorphism variants within tva and tvb receptor genes in Chinese chickens[J]. Poultry Science, 2014, 93(10): 2482-2489. doi: 10.3382/ps.2014-04077
      [20]
      KREAGER K S. Chicken industry strategies for control of tumor virus infections[J]. Poultry Science, 1998, 77(8): 1213-1216. doi: 10.1093/ps/77.8.1213
      [21]
      MENG F, LI Q, ZHANG Y, et al. Characterization of subgroup J avian leukosis virus isolated from Chinese indigenous chickens[J]. Virology Journal, 2018, 15(1): 33. doi: 10.1186/s12985-018-0947-1
      [22]
      LI H, TAN M, ZHANG F, et al. Diversity of Avian leukosis virus subgroup J in local chickens, Jiangxi, China[J]. Scientific Reports, 2021, 11: 4797. doi: 10.1038/s41598-021-84189-7
      [23]
      ADKINS H B, BLACKLOW S C, YOUNG J A. Two functionally distinct forms of a retroviral receptor explain the nonreciprocal receptor interference among subgroups B, D, and E avian leukosis viruses[J]. Journal of Virology, 2001, 75(8): 3520-3526. doi: 10.1128/JVI.75.8.3520-3526.2001
      [24]
      ELLEDER D, STEPANETS V, MELDER D C, et al. The receptor for the subgroup C avian sarcoma and leukosis viruses, Tvc, is related to mammalian butyrophilins, members of the immunoglobulin superfamily[J]. Journal of Virology, 2005, 79(16): 10408-10419. doi: 10.1128/JVI.79.16.10408-10419.2005
      [25]
      CHAI N, BATES P. Na/H exchanger type 1 is a receptor for pathogenic subgroup J avian leukosis virus[J]. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103(14): 5531-5536. doi: 10.1073/pnas.0509785103
      [26]
      LI X, CHEN W, ZHANG H, et al. Naturally occurring frameshift mutations in the tvb receptor gene are responsible for decreased susceptibility of chicken to infection with avian leukosis virus subgroups B, D, and E[J]. Journal of Virology, 2018, 92(8): e01770-e01717.
    • Cited by

      Periodical cited type(1)

      1. 赵薇,曹国伟,周子航,王卫振,辛国省,蔡正云,顾亚玲,张娟. 鸡肉品质相关调控基因研究进展. 农业生物技术学报. 2022(02): 370-378 .

      Other cited types(1)

    Catalog

      Article views (98) PDF downloads (25) Cited by(2)

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return