| Citation: |
LIANG Yi, ZHOU Tong, BAI Ling, et al. Biofilm-forming capacity of soil nitrogen-fixing bacteria and related gene expression under florfenicol combined with copper environmental stress[J]. Journal of South China Agricultural University, 2023, 44(3): 402-409. DOI: 10.7671/j.issn.1001-411X.202205046
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The ecotoxic effects of florfenicol and copper (Cu) residues on soil nitrogen-fixing bacteria were studied to provide a basis for evaluating the environmental risks of veterinary drugs.
A dominant nitrogen-fixing bacterium RpEC2071 was isolated from peanut root enclosure and treated under florfenicol and Cu stress. We set the blank group (0 μg/mL), florfenicol group (40 μg/mL), Cu group (200 μg/mL) and mixed group (flufenicol 40 μg/mL, Cu 200 μg/mL), and collected samples at multiple time points after dosing. The phenol-sulfuric acid method and 96-well microplate method were used to study the effects of florfenicol and Cu alone or in combination on the production of extracellular polysaccharides and biofilm formation of nitrogen-fixing bacterium. RT-qPCR was used to determine nitrogen-fixing bacterium biofilm formation and the mRNA expression levels of nitrogen fixation-related genes.
Florfenicol and Cu alone promoted the formation of biofilms, and the biofilm formation capacity of both was about twice that of the blank group. Under mixed stress, the biofilm formation was inhibited, and the biofilm formation capacity of the blank group was 3.1 times that of it. The results of extracellular polysaccharide secretion were basically consistent with the results of the determination of biofilm formation ability. Biofilm-related genes were significantly positively correlated with nitrogen metabolism regulatory genes, and the addition of florfenicol and Cu changed the expression levels of genes such as fliQ,ntrX andnnrR, and it would produce synergistic or antagonistic effects after the combination treatment of florfenicol and Cu.
The individual or combined stress of florfenicol and Cu affects the expression of biofilm-related genes in nitrogen-fixing bacteria RpEC2071, which in turn affects its ability to form biofilm. This research reveals the ecotoxic potential of veterinary drugs and heavy metal residue stresses on soil nitrogen-fixing bacteria, using florfenicol and Cu as examples. As a result, this can lead to the impairment of the nitrogen-fixing ecosystem in soil.
| [1] |
牛金利, 彭金菊, 明月月, 等. 氟苯尼考胁迫对土壤细菌耐药性的影响[J]. 中国兽医杂志, 2019, 55(4): 95-98.
|
| [2] |
CHOI J Y, KIM Y, KO E A, et al. Acinetobacter species isolates from a range of environments: Species survey and observations of antimicrobial resistance[J]. Diagnostic Microbiology and Infectious Disease, 2012, 74(2): 177-180. doi: 10.1016/j.diagmicrobio.2012.06.023
|
| [3] |
马驿, 彭金菊, 王芸, 等. 环丙沙星对土壤微生物量碳和土壤微生物群落碳代谢多样性的影响[J]. 生态学报, 2013, 33(5): 1506-1512.
|
| [4] |
呼秀智, 薛占永, 王绥华, 等. 氟苯尼考对土壤微生物活动影响研究[J]. 山东畜牧兽医, 2011, 32(2): 3-5.
|
| [5] |
CAI L M, WANG Q S, LUO J, et al. Heavy metal contamination and health risk assessment for children near a large Cu-smelter in central China[J]. Science of the Total Environment, 2019, 650: 725-733. doi: 10.1016/j.scitotenv.2018.09.081
|
| [6] |
施昊坤, 吴次芳, 张茂鑫, 等. 土地整治对工业区周边土壤微生物多样性和群落结构影响分析[J]. 环境科学学报, 2020, 40(1): 212-223.
|
| [7] |
GAO M L, SONG W H, ZHOU Q, et al. Interactive effect of oxytetracycline and lead on soil enzymatic activity and microbial biomass[J]. Environmental Toxicology and Pharmacology, 2013, 36(2): 667-674. doi: 10.1016/j.etap.2013.07.003
|
| [8] |
BAO Y Y, CHEN Q, MA W, et al. Influence of Fe addition on the accumulation of oxytetracycline in rice seedlings (Oryza sativa L. ) growing in hydroponic and soil culture[J]. Journal of Soils and Sediments, 2018, 18(5): 1958-1970. doi: 10.1007/s11368-018-1920-8
|
| [9] |
WANG M, WU J, ZHOU T, et al. Effects of copper and florfenicol on nirS- and nirK-type denitrifier communities and related antibiotic resistance in vegetable soils[J]. Ecotoxicology and Environmental Safety, 2021, 213: 112011. doi: 10.1016/j.ecoenv.2021.112011
|
| [10] |
FALKOWSKI P G. Volution of the nitrogen cycle and its influence on the biological sequestration of CO2 in the ocean[J]. Nature, 1997, 387(6630): 272-275. doi: 10.1038/387272a0
|
| [11] |
鲁文茹, 刘广锋, 郭志勋, 等. 金银花、连翘对溶珊瑚弧菌及其生物膜活性的影响[J]. 广东药学院学报, 2014, 30(3): 297-300.
|
| [12] |
徐光域, 颜军, 郭晓强, 等. 硫酸−苯酚定糖法的改进与初步应用[J]. 食品科学, 2005, 26(8): 342-346.
|
| [13] |
邹晓蕾, 刘礼崔, 罗立新. 细菌总RNA提取方法的比较[J]. 现代食品科技, 2013, 29(8): 1948-1954.
|
| [14] |
KARATAN E, WATNICK P. Signals, regulatory networks, and materials that build and break bacterial biofilms[J]. Microbiology and Molecular Biology Reviews, 2009, 73(2): 310-347. doi: 10.1128/MMBR.00041-08
|
| [15] |
冯世文, 曾芸, 李军, 等. 细菌对氟苯尼考的耐药机制研究[J]. 黑龙江畜牧兽医, 2014(5): 52-54. doi: 10.13881/j.cnki.hljxmsy.2014.0015
|
| [16] |
杜心恬, 宋馨, 刘欣欣, 等. 细菌胞外多糖生物合成转录调控因子研究进展[J]. 微生物学通报, 2021, 48(2): 573-581.
|
| [17] |
BOGINO P C, DE LAS MERCEDES OLIVA M, SORROCHE F G, et al. The role of bacterial biofilms and surface components in plant-bacterial associations[J]. International Journal of Molecular Sciences, 2013, 14(8): 15838-15859. doi: 10.3390/ijms140815838
|
| [18] |
WANG D, XU A M, ELMERICH C, et al. Biofilm formation enables free-living nitrogen-fixing rhizobacteria to fix nitrogen under aerobic conditions[J]. The ISME Journal, 2017, 11(7): 1602-1613. doi: 10.1038/ismej.2017.30
|
| [19] |
LI S, PENG C, CHENG T, et al. Nitrogen-cycling microbial community functional potential and enzyme activities in cultured biofilms with response to inorganic nitrogen availability[J]. Journal of Environmental Sciences, 2019, 76: 89-99. doi: 10.1016/j.jes.2018.03.029
|
| [20] |
YANG Z M, HAN Y L, MA Y, et al. Global investigation of an engineered nitrogen-fixing Escherichia coli strain reveals regulatory coupling between host and heterologous nitrogen-fixation genes[J]. Scientific Reports, 2018, 8(1): 10913-10928. doi: 10.1038/s41598-018-29241-9
|
| [21] |
LI K H, WU G C, LIAO Y L, et al. RpoN1 and RpoN2 play different regulatory roles in virulence traits, flagellar biosynthesis, and basal metabolism in Xanthomonas campestris[J]. Molecular Plant Pathology, 2020, 21(7): 907-922. doi: 10.1111/mpp.12938
|
| [22] |
CALATRAVA M, NOGALES J, AMEZTOY K, et al. The NtrY/NtrX system of Sinorhizobium meliloti GR4 regulates motility, EPS I production, and nitrogen metabolism but is dispensable for symbiotic nitrogen fixation[J]. Molecular Plant-Microbe Interactions, 2017, 30(7): 566-577. doi: 10.1094/MPMI-01-17-0021-R
|
| [23] |
HAO J, FENG Y, WANG X, et al. Soil microbial nitrogen-cycling gene abundances in response to crop diversification: A meta-analysis[J]. Science of the Total Environment, 2022, 838(4): 156621. doi: 10.1016/j.scitotenv.2022.156621.
|
| [24] |
BUENO E, ROBLES E, TORRES M, et al. Disparate response to microoxia and nitrogen oxides of the Bradyrhizobium japonicum napEDABC, nirK and norCBQD denitrification genes[J]. Nitric Oxide, 2017, 68: 137-149. doi: 10.1016/j.niox.2017.02.002
|
| [25] |
BUENO E, MANIA D, MESA S, et al. Regulation of the emissions of the greenhouse gas nitrous oxide by the soybean endosymbiont Bradyrhizobium diazoefficiens[J]. International Journal of Molecular Sciences, 2022, 23(3): 1486. doi: 10.3390/ijms23031486.
|
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