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SUN Yuan, TANG Xiaoyu, BAI Yang, et al. Regulation effects of microRNA-1285 and its target DDX3X on Senecavirus A infected PK-15 cells[J]. Journal of South China Agricultural University, 2023, 44(3): 357-366. DOI: 10.7671/j.issn.1001-411X.202205038
Citation: SUN Yuan, TANG Xiaoyu, BAI Yang, et al. Regulation effects of microRNA-1285 and its target DDX3X on Senecavirus A infected PK-15 cells[J]. Journal of South China Agricultural University, 2023, 44(3): 357-366. DOI: 10.7671/j.issn.1001-411X.202205038

Regulation effects of microRNA-1285 and its target DDX3X on Senecavirus A infected PK-15 cells

More Information
  • Received Date: May 18, 2022
  • Available Online: May 17, 2023
  • Objective 

    To explore the regulation roles of microRNA-1285 (miR-1285) and its target DDX3X in Senecavirus A (SVA) infected PK-15 cells.

    Method 

    By qRT-PCR, double luciferase activity and Western blot, the effects of miR-1285 and its target DDX3X on IFN-β secretion and the RIG-I signaling pathway were studied, and their effects on the expression of SVA 3C protein gene were analyzed.

    Result 

    In SVA infected PK-15 cells, the expression of miR-1285 increased significantly, and there was a negative targeting relationship between miR-1285 and DDX3X. Both miR-1285 and DDX3X promoted the transcription and protein expression of IFN-β. MiR-1285 regulated MAVS and TRAF3 signaling molecules in the RIG-I signaling pathway by targeting DDX3X. For SVA 3C protein, DDX3X significantly inhibited the transcription of 3C and reversed the up-regulation trend induced by miR-1285.

    Conclusion 

    After infecting PK-15 cells with SVA, host miR-1285 and its target DDX3X can regulate the expression of IFN-β and the viral 3C protein, which will lay a foundation for clarifying the molecular mechanism of miRNAs regulating SVA infection, and provide a new scientific basis for the prevention, control and diagnosis of SVA.

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