Functional characterization of phosphorus deficiency-responsive <i>GmNTLs</i> in soybean roots

LI Yaxue; PAN Yaoliang; PENG Guangfen; TIAN Jiang; LU Xing; LIANG Cuiyue

doi:10.7671/j.issn.1001-411X.202204026

Journal of South China Agricultural University > 2023 > 44(2): 221-229. > DOI: 10.7671/j.issn.1001-411X.202204026

LI Yaxue, PAN Yaoliang, PENG Guangfen, et al. Functional characterization of phosphorus deficiency-responsive GmNTLs in soybean roots[J]. Journal of South China Agricultural University, 2023, 44(2): 221-229. DOI: 10.7671/j.issn.1001-411X.202204026

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Functional characterization of phosphorus deficiency-responsive GmNTLs in soybean roots

College of Natural Resources and Environment/Root Biology Center, South China Agricultural University, Guangzhou 510642, China

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Received Date: April 15, 2022
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
Low phosphorus (P) availability and aluminum (Al) toxicity constrain crop production in acid soils. NTL transcription factors play an important role in the mechanisms of plant response to various abiotic stresses, including Al toxicity. This study focused on analyzing the function of GmNTLs in soybean roots responding to P deficiency.

Method
Expression pattern were performed on 15 members of the GmNTL family in the soybean by RT-qPCR assays. We further investigated the function of some GmNTLs members in adaptation to low P by overexpressing the genes of GmNTL1/4/7/8/10/12 in Arabidopsis thaliana.

Result
The phylogenetic analysis and the tissue expression analysis of each subfamily members revealed that 15 GmNTLs were divided into three subgroups and different GmNTL family members had different tissue expression pattern in soybean. The RT-qPCR results showed that the expression levels of GmNTL1/4/7/8/10/12 in soybean roots significantly increased after 12 days of low P treatment. Overexpression of different GmNTLs in Arabidopsis showed different responses to low P. The fresh weight of transgenic Arabidopsis overexpressing GmNTL4/10/12 significantly increased compared to control lines under high P treatment. Overexpression of GmNTL4 significantly improved fresh weight of transgenic Arabidopsis plant under low P deficiency; Whereas, the plant fresh weight of transgene lines overexpressing GmNTL1/12 significantly decreased. Overexpression of GmNTL12 reduced the primary root length of transgenic plants; Whereas, overexpression of other GmNTLs had no significant effect on primary root length.

Conclusion
GmNTLs involve in the response of soybean roots to low P stress, and these results can provide a theoretical basis for cultivating soybean varieties with high P efficiency.
- Glycine max,
- Root,
- Low phosphorus stress,
- GmNTLs,
- NTL transcription factor

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References

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Functional characterization of phosphorus deficiency-responsive GmNTLs in soybean roots