Existing position and extraction of antifungal substance produced by <i>Chaetomium subaffine</i> LB-1

LIU Caiyun; GAO Xiuqing; ZHAO Jing

doi:10.7671/j.issn.1001-411X.202204019

Journal of South China Agricultural University > 2023 > 44(2): 280-286. > DOI: 10.7671/j.issn.1001-411X.202204019

LIU Caiyun, GAO Xiuqing, ZHAO Jing. Existing position and extraction of antifungal substance produced by Chaetomium subaffine LB-1[J]. Journal of South China Agricultural University, 2023, 44(2): 280-286. DOI: 10.7671/j.issn.1001-411X.202204019

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Existing position and extraction of antifungal substance produced by Chaetomium subaffine LB-1

College of Seed and Facility Agricultural Engineering, Weifang Universtiy, Weifang 261061, China

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Received Date: April 11, 2022
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
The existing position and extraction method of antifungal substance produced by Chaetomium subaffine strain LB-1 were studied in order to lay a foundation for the development of the strain LB-1 to control plant diseases.

Method
With Botrytis cinerea and Exserohilum turcicum as test plant pathogens, the sealed plate assay was used to detect whether the strain LB-1 could produce volatile antifungal substance. The hyphae ultrasonic breaking and liquid culture methods were used to detect the existing position of nonvolatile antifungal substance produced by the strain LB-1. The extraction method of antifungal substance was determined by detecting the inhibitory effects of ammonium sulfate precipitation, hydrochloric acid precipitation and organic solvents extracts of strain LB-1 culture broth via poison plate assay and filter paper disc assay.

Result
The strain LB-1 did not have obvious inhibitory effect on the growth of the two test plant pathogens when being co-cultured in a sealed plate with each plant pathogen, indicating that strain LB-1 could not produce volatile antifungal substance. The antifungal activity of the intracellular extract of the strain LB-1 was not different from that of the control, but its culture broth had a strong inhibitory effect on B. cinerea and E. turcicum, indicating that the antifungal substances produced by the strain LB-1 existed outside the mycelium. Neither ammonium sulfate precipitate nor hydrochloric acid precipitate of strain LB-1 culture broth showed inhibitory effect on B. cinerea and E. turcicum, but the organic solvent extract of strain LB-1 culture broth showed antifungal effect, and the inhibition rate of n-butanol extract was the highest. When the concentration was 0.1 mg/mL, the inhibition rates against B. cinerea and E. turcicum growth were 59.80% and 58.37% respectively.

Conclusion
The strain LB-1 inhibited the growth of plant pathogenic fungi by producing extracellular nonvolatile antifungal substances, and the antifungal substance in the culture broth can be extracted by n-butanol.
- Chaetomium subaffine,
- Antifungal substance,
- Culture broth,
- Crude extract of n-butanol

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References (31)

References

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