The inactivation effects of physicochemical factors on African swine fever virus

GONG Lang; SUN Yingshuo; XU Runda; CHEN Xiongnan; GAO Qi; HUANG Zhao; CHEN Xiaojun; ZHENG Jiachen; GUO Yanchen; WU Ruixia; ZENG Fanliang; WANG Heng; ZHANG Guihong

doi:10.7671/j.issn.1001-411X.202203044

Journal of South China Agricultural University > 2023 > 44(3): 348-356. > DOI: 10.7671/j.issn.1001-411X.202203044

GONG Lang, SUN Yingshuo, XU Runda, et al. The inactivation effects of physicochemical factors on African swine fever virus[J]. Journal of South China Agricultural University, 2023, 44(3): 348-356. DOI: 10.7671/j.issn.1001-411X.202203044

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The inactivation effects of physicochemical factors on African swine fever virus

College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China

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Received Date: March 22, 2022
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
The purpose of this research was to understand the biological characteristics and physicochemical resistance of African swine fever virus (ASFV) in China, so as to improve the biosafety level of pig farms for the prevention and control of African swine fever.

Method
Hemadsorption test and qPCR test were used to verify the inactivation effects of different physicochemical factors (including static treatment, temperature, UVC irradiation, indoor and outdoor drying, sun exposure and disinfectant) on ASFV.

Result
UVC irradiation for 30 min could inactivate the virus, the longer the irradiation time, the more serious degradation of ASFV nucleic acid. Indoor drying for 2.5 d, outdoor drying for 1.5 d or sun exposure for 30 min could inactivate ASFV, but these three physical factors had no effect on ASFV nucleic acid degradation. ASFV was susceptible to common disinfectants tested in study, except iodate mixed solution, all disinfectants could completely inactivate ASFV when treated at room temperature at the recommended diluted concentration for 15 or 30 min. An increase in temperature (4, 25 and 37 ℃) enhanced the inactivation effect of disinfectants. The presence of organic compound FBS could weaken the effect of disinfectants, and an increase in the volume fraction of FBS (0, 10% and 30%) could reduce the inactivation effect.

Conclusion
This paper systematically studies the effects of common physicochemical factors on the inactivation of ASFV, which is helpful to fully understand the biological characteristics of ASFV, and has important guiding significance for clinical prevention and control of African swine fever.
- African swine fever virus,
- Physicochemical resistance,
- Disinfectant,
- Virus inactivation,
- Nucleic acid degradation

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References (17)

References

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The inactivation effects of physicochemical factors on African swine fever virus

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