Objective The purpose of this research was to understand the biological characteristics and physicochemical resistance of African swine fever virus (ASFV) in China, so as to improve the biosafety level of pig farms for the prevention and control of African swine fever.
Method Hemadsorption test and qPCR test were used to verify the inactivation effects of different physicochemical factors (including static treatment, temperature, UVC irradiation, indoor and outdoor drying, sun exposure and disinfectant) on ASFV.
Result UVC irradiation for 30 min could inactivate the virus, the longer the irradiation time, the more serious degradation of ASFV nucleic acid. Indoor drying for 2.5 d, outdoor drying for 1.5 d or sun exposure for 30 min could inactivate ASFV, but these three physical factors had no effect on ASFV nucleic acid degradation. ASFV was susceptible to common disinfectants tested in study, except iodate mixed solution, all disinfectants could completely inactivate ASFV when treated at room temperature at the recommended diluted concentration for 15 or 30 min. An increase in temperature (4, 25 and 37 ℃) enhanced the inactivation effect of disinfectants. The presence of organic compound FBS could weaken the effect of disinfectants, and an increase in the volume fraction of FBS (0, 10% and 30%) could reduce the inactivation effect.
Conclusion This paper systematically studies the effects of common physicochemical factors on the inactivation of ASFV, which is helpful to fully understand the biological characteristics of ASFV, and has important guiding significance for clinical prevention and control of African swine fever.
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