Establishment of IMSA-LAMP detection method for <i>Ralstonia solanacearu</i>

YUAN Ting; LUO Longhui; ZHANG Xueyin; LIU Jiping

doi:10.7671/j.issn.1001-411X.202203030

Journal of South China Agricultural University > 2022 > 43(4): 89-98. > DOI: 10.7671/j.issn.1001-411X.202203030

YUAN Ting, LUO Longhui, ZHANG Xueyin, et al. Establishment of IMSA-LAMP detection method for Ralstonia solanacearu[J]. Journal of South China Agricultural University, 2022, 43(4): 89-98. DOI: 10.7671/j.issn.1001-411X.202203030

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Establishment of IMSA-LAMP detection method for Ralstonia solanacearu

College of Animal Science, South China Agricultural University/Regional Sericulture Training Center For Asia-Pacific, Guangzhou 510642, China

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Received Date: March 08, 2022
Available Online: May 17, 2023

Abstract

Abstract

Objective
Mulberry bacterial wilt is a seriously harmful bacterial disease caused by Ralstonia solanacearum. Therefore, it is of great significance to establish a rapid and sensitive detection method for R. solanacearum to effectively control mulberry bacterial wilt.

Method
Pectate lyase gene of R. solanacearum was used as the target. Based on the primer design principle of isothermal multiple self-matching-initiated amplification (IMSA), combining the loop-mediated isothermal amplification (LAMP) reaction system, a rapid and effective IMSA-LAMP method for detection ofR. solanacearum was established. The optimal reaction parameters of this method were screened.

Result
The IMSA-LAMP method based on pectate lyase gene could complete the specific detection of positive samples within 45 min at 64.5 ℃, and the detection sensitivity of R. solanacearum template DNA was 200 fg/μL (the corresponding bacteria detection sensitivity was 1 × 10² CFU/mL); The detection rate of suspected mulberry bacterial wilt samples collected in production was 87.5%.

Conclusion
This method has good practicability and can provide new technical support for the rapid detection, diagnosis and epidemic prevention of mulberry bacterial wilt.
- Mulberry bacterial wilt,
- IMSA-LAMP,
- Ralstonia solanacearum,
- Pectate lyase gene

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References (43)

References

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Corresponding author: LIU Jiping, liujiping@scau.edu.cn

College of Animal Science, South China Agricultural University/Regional Sericulture Training Center For Asia-Pacific, Guangzhou 510642, China

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Establishment of IMSA-LAMP detection method for Ralstonia solanacearu

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Corresponding author: LIU Jiping, liujiping@scau.edu.cn

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