Identification of the pathogen causing <i>Trichosanthes kirilowii</i> fruit rot and screening of fungicides

WANG Qian; TONG Rui; TAN Genjia; ZHANG Lixin

doi:10.7671/j.issn.1001-411X.202202006

Journal of South China Agricultural University > 2023 > 44(2): 270-279. > DOI: 10.7671/j.issn.1001-411X.202202006

WANG Qian, TONG Rui, TAN Genjia, et al. Identification of the pathogen causing Trichosanthes kirilowii fruit rot and screening of fungicides[J]. Journal of South China Agricultural University, 2023, 44(2): 270-279. DOI: 10.7671/j.issn.1001-411X.202202006

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Identification of the pathogen causing Trichosanthes kirilowii fruit rot and screening of fungicides

WANG Qian^{1, 2,},
TONG Rui^1,,
TAN Genjia¹,
ZHANG Lixin^1, ,

1.
College of Plant Protection/Anhui Province Key Laboratory of Integrated Pest Management on Crops, Anhui Agricultural University, Hefei 230036, China
2.
Institute of Germplasm Resources and Biotechnology, Tianjin Academy of Agricultural Sciences, Tianjin 300381, China

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Received Date: February 09, 2022
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To clarify the pathogen causing Trichosanthes kirilowii fruit rot and screen effective fungicides for controlling the disease.

Method
Samples of rot fruit from T. kirilowii plants were collected from Dabie Mountain Areas, Anhui Province in 2019—2020. A total of 35 representative isolates were obtained by tissue isolation. The pathogenicity was verified according to the Koch’s postulate, and the causal agents were identified based on the morphological characteristics and multilocus sequence analysis. The indoor toxicity of seven fungicides against the pathogen was detected by the mycelium growth rate method.

Result
Colonies with four different morphological characteristics were isolated and purified. The isolates were confirmed to be pathogenic to the fruit of T. kirilowii according to the Koch’s postulate. They were identified as Fusarium fujikuroi, F. proliferatum, Colletotrichum fructicola and C. liaoningense by combining morphological characteristics with phylogenetic analysis, with isolation frequencies of 31.4%, 8.6%, 20.0% and 40.0%, respectively. In addition, the indoor toxicity test results indicated that prochloraz, fludioxonil, difenoconazole, phenamacril and chlorothalonil had better inhibitory effect against F. fujikuroi andF. proliferatum, with EC₅₀ ranging from 0.1046 to 5.1781 μg/mL. Prochloraz, fludioxonil and difenoconazole had better inhibitory effect againstC. fructiicola and C. liaoningense with EC₅₀ ranging from 0.0097 to 2.1325 μg/mL.

Conclusion
The causal agents associated with fruit rot disease on T. kirilowii are Fusarium spp. and Colletotrichum spp.. Prochloraz, fludioxonil and difenoconazole all show good inhibitory activity against the pathogens.
- Trichosanthes kirilowii,
- Fruit rot disease,
- Fusarium,
- Colletotrichum,
- Pathogenicity,
- Fungicide screening

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References (29)

References

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