Acute toxic effect of glyphosate on <i>Pomacea canaliculata</i> under the condition of oxidative deposition

LIANG Dongxia; LI Yinshi; LI Shuoyu; GONG Maojian; QIAN Jiuli; LI Huashou

doi:10.7671/j.issn.1001-411X.202111011

Journal of South China Agricultural University > 2022 > 43(4): 67-76. > DOI: 10.7671/j.issn.1001-411X.202111011

LIANG Dongxia, LI Yinshi, LI Shuoyu, et al. Acute toxic effect of glyphosate on Pomacea canaliculata under the condition of oxidative deposition[J]. Journal of South China Agricultural University, 2022, 43(4): 67-76. DOI: 10.7671/j.issn.1001-411X.202111011

Citation:

PDF (777 KB)

Acute toxic effect of glyphosate on Pomacea canaliculata under the condition of oxidative deposition

1.
Guangdong Provincial Key Laboratory of Eco-Circular Agriculture/Guangdong Engineering Research Center for Modern Eco-Agriculture and Circular Agriculture/Key Laboratory of Agro-Environment in the Tropics, Ministry of Agriculture of China/ College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China
2.
Guangdong Engineering Polytechnic, Guangzhou 510520, China

More Information

Received Date: November 10, 2021
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To explore the acute toxic effect of glyphosate on Pomacea canaliculata under the combined pollution of glyphosate and H₂O₂.

Method
Using the hydrostatic biological test method, adult Pomacea canaliculata with uniform growth in the field were collected, and exposed to water with different concentrations of glyphosate and similar to the average concentration of H₂O₂ (50 μmol·L⁻¹) in precipitation of Guangzhou. The influences of glyphosate on some water quality indexes of P. canaliculata habitat and acute toxic effects of P. canaliculata under oxidative deposition conditions were studied.

Result
For the water quality index, oxidation-reduction potential (ORP) increased with the increase of exposure time, from 330 mV to about 540 mV; The change of pH with the increase of exposure time increased or decreased under different treatments, and finally maintained between 7.0 and 8.5; The concentration of dissolved oxygen (DO) was between 8.5 and 16.0 mg·L⁻¹, and there was no obvious variation trend. The three water quality indexes above were within the range that the growth of P. canaliculata could adapt to. Acute toxicity tests indicated that glyphosate had low toxicity to P. canaliculata, and the 50% lethal concentration (LC₅₀) in 48 h was 133.479 mg·L⁻¹ with or without H₂O₂ deposition. The anti-oxidative stress enzymes of superoxide dismutase (SOD) and catalase (CAT), as well as energy metabolism-related enzymes of amylase (AMS) and lipase (LPS) in P. canaliculata were firstly up-regulated, then suppressed, and finally adapted to up-regulation. It showed that in the process of poisoning, P. canaliculata could slowly adapt to the stress environment and continuously improve its stress resistance, so that it could survive normally in this stress.

Conclusion
Under the condition of oxidative deposition, the acute toxic effect of glyphosate on P. canaliculata is low, and the activities of the four related typical enzymes are generally up-regulated. It is ineffective and unrealistic to try to inhibit P. canaliculata through herbicide weeding at the same time.
- Glyphosate,
- Pomacea canaliculata,
- Hydrogen peroxide,
- Acute toxicity,
- Enzymatic activity

FullText(HTML)

References (54)

References

[1]	赵永超, 严志会, 吴帆, 等. 福寿螺卵巢发育与卵子发生的组织学研究[J]. 华南农业大学学报, 2016, 37(3): 95-99.
[2]	赵兰, 骆世明, 黎华寿, 等. 不同浓度下四种除草剂对福寿螺和坑螺的生态毒理效应[J]. 生态学报, 2011, 31(19): 5720-5727.
[3]	沈路遥, 彭自然, 戴智. 草甘膦水生生物毒性、环境行为、检测方法研究进展[J]. 农药, 2020, 59(1): 6-10.
[4]	周垂帆, 李莹, 张晓勇, 等. 草甘膦毒性研究进展[J]. 生态环境学报, 2013, 22(10): 1737-1743. doi: 10.3969/j.issn.1674-5906.2013.10.016
[5]	MYERS J P, ANTONIOU M N, BLUMBERG B, et al. Concerns over use of glyphosate-based herbicides and risks associated with exposures: A consensus statement[J]. Environmental Health, 2016, 15: 19. doi: 10.1186/s12940-016-0117-0.
[6]	FANTÓN N, BACCHETTA C, ROSSI A, et al. Effects of a glyphosate-based herbicide on the development and biochemical biomarkers of the freshwater copepod Notodiaptomus carteri (Lowndes, 1934)[J]. Ecotoxicology and Environmental Safety, 2020, 196: 110501. doi: 10.1016/j.ecoenv.2020.110501.
[7]	VELASQUES R R, SANDRINI J Z, DA ROSA C E. Roundup^® in zebrafish: Effects on oxidative status and gene expression[J]. Zebrafish, 2016, 13(5): 432-441. doi: 10.1089/zeb.2016.1259
[8]	DE MELO TAROUCO F, DE GODOI F G A, Velasques R R, et al. Effects of the herbicide roundup on the polychaeta Laeonereis acuta: Cholinesterases and oxidative stress.[J]. Ecotoxicology and Environmental Safety, 2017, 135: 259-266. doi: 10.1016/j.ecoenv.2016.10.014
[9]	PÉREZ L G, VERA M S, MIRANDA L A. Effects of herbicide glyphosate and glyphosate- based formulations on aquatic ecosystems[M]//Kortekamp A. Herbicides and Environment. [s.l.]: InTech, 2011: 343-368.
[10]	SÁNCHEZ J A A, JUNIOR A S V, CORCINI C D, et al. Effects of Roundup formulations on biochemical biomarkers and male sperm quality of the livebearing Jenynsia multidentata[J]. Chemosphere, 2017, 177: 200-210. doi: 10.1016/j.chemosphere.2017.02.147
[11]	廖艺钰, 惠吕佳, 严吉祥, 等. 草甘膦农药对斑马鱼的急性毒性和慢性毒性研究[J]. 广州化工, 2020, 48(21): 66-68. doi: 10.3969/j.issn.1001-9677.2020.21.024
[12]	SANTADINO M, COVIELLA C, MOMO F. Glyphosate sublethal effects on the population dynamics of the earthworm Eisenia fetida (Savigny, 1826)[J]. Water, Air & Soil Pollution, 2014, 225(12): 1-8.
[13]	LE DU-CARRÉE J, BOUKHARI R, CACHOT J, et al. Generational effects of a chronic exposure to a low environmentally relevant concentration of glyphosate on rainbow trout, Oncorhynchus mykiss[J]. Science of the Total Environment, 2021, 801: 149462. doi: 10.1016/j.scitotenv.2021.149462.
[14]	WEE J, LEE Y, KIM Y, et al. Multigeneration toxicity of Geunsami® (a glyphosate-based herbicide) to Allonychiurus kimi (Lee) (Collembola) from sub-individual to population levels[J]. Environmental Pollution, 2021, 291: 118172. doi: 10.1016/j.envpol.2021.118172.
[15]	XU Y G, LI A J, LI K B, et al. Effects of glyphosate-based herbicides on survival, development and growth of invasive snail ( Pomacea canaliculata )[J]. Aquatic Toxicology, 2017, 193: 136-143. doi: 10.1016/j.aquatox.2017.10.011
[16]	刘聪辉. 基于多组学数据对福寿螺生物入侵机制的探究[D]. 北京: 中国农业科学院, 2018.
[17]	于瑞新, 刘旻霞, 李亮, 等. 长三角地区近15年大气臭氧柱浓度时空变化及影响因素[J]. 环境科学学报, 2021, 41(3): 770-784.
[18]	LIU Z R, WANG Y S, HU B, et al. Elucidating the quantitative characterization of atmospheric oxidation capacity in Beijing, China[J]. Science of the Total Environment, 2021, 771: 145306. doi: 10.1016/j.scitotenv.2021.145306.
[19]	ZHAO D D, LIU G J, XIN J Y, et al. Haze pollution under a high atmospheric oxidization capacity in summer in Beijing: Insights into formation mechanism of atmospheric physicochemical processes[J]. Atmospheric Chemistry and Physics, 2020, 20(8): 4575-4592. doi: 10.5194/acp-20-4575-2020
[20]	YUAN J C, SHILLER A M. The variation of hydrogen peroxide in rainwater over the South and Central Atlantic Ocean[J]. Atmospheric Environment, 2000, 34(23): 3973-3980. doi: 10.1016/S1352-2310(00)00167-9
[21]	GON ÇALVES C, DOS SANTOS M A, FORNARO A, et al. Hydrogen peroxide in the rainwater of sao paulo megacity: Measurements and controlling factors[J]. Journal of the Brazilian Chemical Society, 2010, 21(2): 331-339. doi: 10.1590/S0103-50532010000200020
[22]	COOPER W J, SALTZMAN E S, ZIKA R G. The contribution of rainwater to variability in surface ocean hydrogen peroxide[J]. Journal of Geophysical Research, 1987, 92(C3): 2970-2980. doi: 10.1029/JC092iC03p02970
[23]	魏西会, 刘素美, 张经, 等. 天然水体中微量过氧化氢的测定方法[J]. 海洋科学, 2008, 32(10): 96-100.
[24]	WILLEY J D, KIEBER R J, LANCASTER R D. Coastal rainwater hydrogen peroxide: Concentration and deposition[J]. Journal of Atmospheric Chemistry, 1996, 25(2): 149-165. doi: 10.1007/BF00053789
[25]	COOPER W J, ZIKA R G, PETASNE R G, et al. Photochemical formation of hydrogen peroxide in natural waters exposed to sunlight[J]. Environmental Science & Technology, 1988, 22(10): 1156-1160.
[26]	LIN X Y, HE L X, ZHANG R H, et al. Rainwater in Guangzhou, China: Oxidizing properties and physicochemical characteristics[J]. Atmospheric Pollution Research, 2019, 10(1): 303-312. doi: 10.1016/j.apr.2018.08.005
[27]	中华人民共和国国家质量监督检验检疫总局. 化学农药环境安全评价试验准则: 第21部分: 大型甲壳类生物毒性试验: GB/T 31270.21—2014[S]. 北京: 国家标准化委员会, 2014.
[28]	田春美, 钟秋平. 超氧化物歧化酶的现状研究进展[J]. 中国热带医学, 2005, 5(8): 1730-1732. doi: 10.3969/j.issn.1009-9727.2005.08.072
[29]	韩微. 福寿螺对逆境胁迫的行为反应及生理生化反应研究[D]. 南宁: 广西大学, 2012.
[30]	朱丽霞, 黄瑶瑶, 张泽宏, 等. pH、食物和光周期对福寿螺生长发育和繁殖的影响[J]. 生态学报, 2015, 35(8): 2643-2651.
[31]	廖辉杰, 范德江, 逄悦, 等. 河水与海水混合过程中Eh-pH的变化特征及影响因素[J]. 中国海洋大学学报(自然科学版), 2021, 51(4): 100-108.
[32]	谢红梅, 刘泷霞. 新型复合钝化剂对上覆水体及底泥pH-Eh-COD的影响[J]. 广东化工, 2021, 48(10): 127-129. doi: 10.3969/j.issn.1007-1865.2021.10.046
[33]	董煜, 邹联沛, 张晓岚. 苏州河上海市区段底泥有机物的初步研究[J]. 环境科学与技术, 2005, 28(S2): 14-15.
[34]	孟秀柔, 宋青梅, 王飞, 等. 草铵膦和草甘膦在水环境中的行为和毒性效应研究进展[J]. 生态毒理学报, 2021, 16(3): 144-154.
[35]	LUSHCHAK V I, VIISHYN T M, HUSAK V V, et al. Pesticide toxicity: A mechanistic approach[J]. Excli Journal, 2018, 17: 1101-1136.
[36]	STALEY Z R, HARWOOD V J, ROHR J R. A synthesis of the effects of pesticides on microbial persistence in aquatic ecosystems[J]. Critical Reviews in Toxicology, 2015, 45(10): 813-836. doi: 10.3109/10408444.2015.1065471
[37]	DAMALAS C A, ELEFTHEROHORINOS I G. Pesticide exposure, safety issues, and risk assessment indicators[J]. International Journal of Environmental Research and Public Health, 2011, 8(5): 1402-1419. doi: 10.3390/ijerph8051402
[38]	刘昌平, 刘昌利, 王进. 四种常见农药对日本三角涡虫的急性毒性研究[J]. 中国林副特产, 2008(6): 19-22. doi: 10.3969/j.issn.1001-6902.2008.06.006
[39]	CAVALCANTE D G S M, MARTINEZ C B R, SOFIA S H. Genotoxic effects of roundup on the fish Prochilodus lineatus[J]. Mutation Research, 2008, 655(1/2): 41-46.
[40]	GUILHERME S, GAIVÃO I, SANTOS M A, et al. DNA damage in fish (Anguilla anguilla) exposed to a glyphosate-based herbicide: Elucidation of organ-specificity and the role of oxidative stress[J]. Mutation Research: Genetic Toxicology and Environmental Mutagenesis, 2012, 743(1/2): 1-9.
[41]	CUHRA M, TRAAVIK T, BØHN T. Clone- and age-dependent toxicity of a glyphosate commercial formulation and its active ingredient in Daphnia magna[J]. Ecotoxicology, 2013, 22(2): 251-262. doi: 10.1007/s10646-012-1021-1
[42]	陈瑞曦. 超氧化物歧化酶研究及在海洋渔业中的应用[J]. 中国渔业经济, 2014, 32(5): 85-91. doi: 10.3969/j.issn.1009-590X.2014.05.013
[43]	游金进. 过氧化氢酶在养猪生产中的应用[J]. 畜禽业, 2019, 30(5): 21-22.
[44]	宋超, 陈家长, 胡庚东, 等. 除草剂氟乐灵及其降解过程对斑马鱼氧化应激状况的影响[J]. 生态环境学报, 2017, 26(3): 468-472.
[45]	黄秀枝, 蔡瑷安, 尚战峰, 等. 甲氨基阿维菌素胁迫下福寿螺肝脏抗氧化酶活性及显微结构的变化[J]. 江苏农业学报, 2015, 31(3): 552-557. doi: 10.3969/j.issn.1000-4440.2015.03.014
[46]	彭立凤, 赵汝淇, 谭天伟. 微生物脂肪酶的应用[J]. 食品与发酵工业, 2000, 26(3): 68-73. doi: 10.3321/j.issn:0253-990X.2000.03.014
[47]	姜鹤, 孔繁东, 刘兆芳. 温度、pH及金属离子对大连虾蛄消化酶活性影响[J]. 中国食品添加剂, 2016(2): 100-105. doi: 10.3969/j.issn.1006-2513.2016.02.011
[48]	罗志刚, 杨景峰, 罗发兴. α−淀粉酶的性质及应用[J]. 食品研究与开发, 2007, 28(8): 163-167. doi: 10.3969/j.issn.1005-6521.2007.08.053
[49]	罗明珠, 章家恩, 胡九龙, 等. 福寿螺和田螺消化酶活性比较[J]. 生态学报, 2015, 35(11): 3580-3587.
[50]	MODESTO K A, MARTINEZ C B R. Effects of roundup transorb on fish: Hematology, antioxidant defenses and acetylcholinesterase activity[J]. Chemosphere, 2010, 81(6): 781-787. doi: 10.1016/j.chemosphere.2010.07.005
[51]	LAJMANOVICH R C, ATTADEMO A M, PELTZER P M, et al. Toxicity of four herbicide formulations with glyphosate on Rhinella arenarum (Anura: Bufonidae) tadpoles: B-esterases and glutathione S-transferase inhibitors[J]. Archives of Environmental Contamination and Toxicology, 2011, 60(4): 681-689. doi: 10.1007/s00244-010-9578-2
[52]	何铭谦, 罗明珠, 章家恩, 等. 广东福寿螺暴发危害状况调查及防治对策[J]. 贵州农业科学, 2011, 39(1): 100-104. doi: 10.3969/j.issn.1001-3601.2011.01.029
[53]	HORGON F G, FELIX M I, PORTALANZA D, et al. Responses by farmers to the apple snail invasion of Ecuador’s rice fields and attitudes toward predatory snail kites[J]. Crop Protection, 2014, 62: 135-143. doi: 10.1016/j.cropro.2014.04.019
[54]	桑松, 王培丹, 曹宇科, 等. 山坑螺和田螺作为指示生物监测茶园环境农药残留的研究[J]. 华南农业大学学报, 2014, 35(3): 58-62. doi: 10.7671/j.issn.1001-411X.2014.03.011

Cited By

Cited by

Periodical cited type(1)

谭茵，周子豪，黄锦龙，武正军. 福寿螺属主要入侵种(Pomacea canaliculata和Pomacea maculate)研究趋势可视化. 南京师大学报(自然科学版). 2023(01): 88-99 .

Other cited types(0)

Get Citation

PDF

XML

Article views (159) PDF downloads (354) Cited by(1)

Turn off MathJax

Article Contents

Abstract

References

Acute toxic effect of glyphosate on Pomacea canaliculata under the condition of oxidative deposition

Abstract

References

Cited by

Periodical cited type(1)

Other cited types(0)

Catalog

Export File

Citation

Format

Content