Rhizosphere colonization of <i>Bacillus subtilis</i> biocontrol strain SEM-9 and the effect on microbial diversity in rhizosphere soil

LI Qingrong; XING Dongxu; XIAO Yang; LIAO Sentai; ZOU Yuxiao; LIU Fan; LI Erna; ZHOU Donglai; YANG Qiong

doi:10.7671/j.issn.1001-411X.202107008

Journal of South China Agricultural University > 2022 > 43(4): 82-88. > DOI: 10.7671/j.issn.1001-411X.202107008

LI Qingrong, XING Dongxu, XIAO Yang, et al. Rhizosphere colonization of Bacillus subtilis biocontrol strain SEM-9 and the effect on microbial diversity in rhizosphere soil[J]. Journal of South China Agricultural University, 2022, 43(4): 82-88. DOI: 10.7671/j.issn.1001-411X.202107008

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Rhizosphere colonization of Bacillus subtilis biocontrol strain SEM-9 and the effect on microbial diversity in rhizosphere soil

LI Qingrong^{1, 2,},
XING Dongxu^{1, 2},
XIAO Yang^{1, 2},
LIAO Sentai¹,
ZOU Yuxiao¹,
LIU Fan¹,
LI Erna¹,
ZHOU Donglai¹,
YANG Qiong^{1, 2, ,}

1.
The Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China
2.
Key Laboratory of Urban Agriculture in South China, Ministry of Agriculture, Guangzhou 510610, China

More Information

Received Date: July 06, 2021
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
In order to study the colonization rule of Bacillus subtilis biocontrol strain SEM-9 in the rhizosphere of crops and its influence on the microbial diversity of rhizosphere soil.

Method
The strain SEM-9 was labeled with green fluorescent protein by natural transformation method, and the colonization in rhizosphere soil, on root surface and in root tissue were observed by inverted fluorescence microscope. The changes of microbial diversity in rhizosphere soil treated with the strain SEM-9 were analyzed by high-throughput sequencing using soil with soilborne diseases as test material.

Result
The recombinant strain SEM-9-pGFP₂₂ stably expressing green fluorescent protein was constructed. The observations of fluorescence microscopy showed that SEM-9-pGFP₂₂ could colonize on the rhizosphere soil and root surface, but not in root tissue or cell. After treated with the SEM-9 suspension, the incidence rate of cucumber soilborne disease significantly reduced, and the fungal diversity in rhizosphere soil increased.

Conclusion
The GFP labeling method of SEM-9 strain was successfully established, and the rhizosphere colonization rule of the strain and the control effect on cucumber soilborne diseases were clarified, which lays a foundation for the later development of alternative microbial fertilizer.
- Biocontrol strain,
- Rhizosphere colonization,
- Rhizosphere microorganism,
- Microbial diversity

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References (22)

References

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Rhizosphere colonization of Bacillus subtilis biocontrol strain SEM-9 and the effect on microbial diversity in rhizosphere soil

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