- Chinese Core Journal
- Chinese Science Citation Database (CSCD) Source journal
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| Citation: |
FAN Jiaqi, JIA Fang, LI Yusi, et al. Effects of astragalus polysaccharide and astragaloside IV on lipopolysaccharides-induced inflammation of bovine mammary epithelial cells[J]. Journal of South China Agricultural University, 2022, 43(4): 16-28. DOI: 10.7671/j.issn.1001-411X.202106034
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To investigate the effects of astragaloside IV (AS-IV) and astragalus polysaccharides (APS) of astragalus extract on the lipopolysaccharides (LPS)-induced inflammation of bovine mammary epithelial cells (BMECs) and the effect on the expression of β-casein in inflammatory cells.
APS and AS-IV were used to intervene LPS-induced BMECs to detect the changes of cell inflammatory factors, oxidative factors, apoptosis factors and β-casein.
CCK-8 screening found the concentrations of 1g/L APS and 50, 75, 100 mg/L AS-IV were optimal to stimulate BMECs. After stimulating BMECs with 0.5 mg/L LPS for 24 h, the inflammatory cell model was successfully established. Experiments found that AS-IV and APS could significantly inhibit the expression of lactate dehydrogenase (LDH) in inflammatory cells and alleviate the continuous damage of cell membranes in the inflammatory state; Significantly inhibit the expression of malondialdehyde (MDA) and reactive oxygen species (ROS) in inflammatory cells and alleviate the oxidative damage of cells; Significantly inhibit the expression of IL-6, IL-8, TNF-α inflammatory factors in inflammatory cells, and reduce the inflammatory response of cells; Significantly inhibit the expression of apoptotic proteins in inflammatory cells and inhibit cell apoptosis; Significantly activate β-casein in BMECs inflammatory cells and normal cells, and inhibit the expression of TGF-β and ERK1/2.
AS-IV and APS can inhibit the inflammatory response of BMECs induced by LPS and activate the expression of β-casein in inflammatory cells at the optimal concentrations.
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