Effect of <i>ETV5</i> knockout on gene expression profile in mouse muscle

WAN Weican; ZHANG Xianyu; ZHAO Xin; LI Bin; CAI Gengyuan; YANG Huaqiang; XU Zheng

doi:10.7671/j.issn.1001-411X.202106023

Journal of South China Agricultural University > 2022 > 43(2): 26-33. > DOI: 10.7671/j.issn.1001-411X.202106023

WAN Weican, ZHANG Xianyu, ZHAO Xin, et al. Effect of ETV5 knockout on gene expression profile in mouse muscle[J]. Journal of South China Agricultural University, 2022, 43(2): 26-33. DOI: 10.7671/j.issn.1001-411X.202106023

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Effect of ETV5 knockout on gene expression profile in mouse muscle

College of Animal Science, South China Agricultural University, Guangzhou 510642, China

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Received Date: June 15, 2021
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
Compared with the wild type mice, the homozygous ETV5 knockout mice prepared by CRISPR/Cas9 technology showed significant weakness in body size and body weight along with endogenous spermatogonial stem cell ablation. The purpose of this study was to explore the effect of ETV5 knockout on the muscle expression profile of mice.

Method
The muscle tissues of three wild-type male mice and three ETV5 homozygous knockout male mice aged six weeks were collected and total RNA was extracted for transcriptome sequencing. We analyzed the sequencing results using bioinformatic method. Cluster analysis, GO and KEGG enrichment analyses were performed on the differentially expressed genes in the muscle samples of two groups of mice.

Result
A total of 574 differentially expressed genes were screened out from the muscle tissues of two groups, including 292 up-regulated genes and 282 down-regulated genes. Several genes were found to affect the growth and development of ETV5 knockout mice. These genes included Amd1 affecting muscle development of mice, and Chrna2 affecting fat accumulation. Most of the pathways enriched by GO and KEGG analyses were related to fat metabolism and growth and development.

Conclusion
These results provide an explanation for the molecular mechanism of abnormal development of ETV5 knockout mice, and provide references for further study of in vivo function of ETV5 gene.
- ETV5,
- Transcriptome sequencing,
- Mouse,
- Muscular development,
- Gene knockout,
- Gene expression profile

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References

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Effect of ETV5 knockout on gene expression profile in mouse muscle

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