Screening and identification of diuron-degrading strain SL-6 and optimization of degradation conditions

WANG Haodong; ZHANG Jiayu; QIAN Cancan; WEI Zihan; WU Cailan; YANG Desong

doi:10.7671/j.issn.1001-411X.202104010

Journal of South China Agricultural University > 2022 > 43(1): 94-101. > DOI: 10.7671/j.issn.1001-411X.202104010

WANG Haodong, ZHANG Jiayu, QIAN Cancan, et al. Screening and identification of diuron-degrading strain SL-6 and optimization of degradation conditions[J]. Journal of South China Agricultural University, 2022, 43(1): 94-101. DOI: 10.7671/j.issn.1001-411X.202104010

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Screening and identification of diuron-degrading strain SL-6 and optimization of degradation conditions

College of Agriculture, Shihezi University / Key Laboratory for Oasis Agricultural Pest Management and Plant Resource Utilization, Shihezi 832000, China

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Received Date: April 08, 2021
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
This study was aimed to clarify the biological classification of the diuron-degrading strain SL-6 obtained by screening and isolation, and optimize its degradation conditions for providing a new way to degrade diuron.

Method
Diuron-degrading strain SL-6 was isolated in the soil of cotton field by enrichment culture, and identified by 16S rDNA and nrdA gene sequence analysis combined with morphological, physiological and biochemical characteristics. HPLC method was used to detect the degradation effect of SL-6 strain on diuron, and the degradation abilities of the strain under different initial concentrations of diuron, inoculation amount, sucrose content, pH and temperature conditions were studied and the degradation conditions were optimized.

Result
Seven strains were isolated from soil of cotton field. Among them, Achromobacter strains SL-6, SL-7 and SL-9 had good degradation effects on diuron and the degradation kinetics conformed to the degradation kinetics equation. Among the three strains, A. xylosoxidans SL-6 had the best degradation effect, with the degradation rate of 94.6% on the 15th day. When the initial concentration of diuron was 200 mg/L, inoculation amount was 15% (φ), carbon source was not added, pH was 8.0 and temperature was 30 ℃, the degradation rate reached 93.1% after five days.

Conclusion
Strain SL-6 can efficiently degrade diuron, and can be used as a new strain resource. This study provide a basis for further research on microbial degradation of diuron.
- Diuron,
- Biodegradation,
- Bioremediation,
- Achromobacter xylosoxidans,
- Cultural condition

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References (21)

References

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Screening and identification of diuron-degrading strain SL-6 and optimization of degradation conditions