Development of ERA detection method for African swine fever virus based on B646L, EP402R and MGF360/505 genes

ZENG Yuchen; GONG Lang; WANG Jingyu; PAN Haoming; LIANG Xingling; MA Jun; ZHANG Guihong; WANG Heng

doi:10.7671/j.issn.1001-411X.202101031

Journal of South China Agricultural University > 2021 > 42(5): 33-40. > DOI: 10.7671/j.issn.1001-411X.202101031

ZENG Yuchen, GONG Lang, WANG Jingyu, et al. Development of ERA detection method for African swine fever virus based on B646L, EP402R and MGF360/505 genes[J]. Journal of South China Agricultural University, 2021, 42(5): 33-40. DOI: 10.7671/j.issn.1001-411X.202101031

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Development of ERA detection method for African swine fever virus based on B646L, EP402R and MGF360/505 genes

1.
College of Veterinary medicine, South China Agricultural University/Key Laboratory of Comprehensive Prevention and Control for Severe Clinical Animal Diseases of Guangdong Province, Guangzhou 510642, China
2.
African Swine Fever Regional Laboratory of China (Guangzhou)/Prevention and Control Technology Research Center for African Swine Fever, South China Agricultural University, Guangzhou 510642, China
3.
National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China

More Information

Received Date: January 14, 2021
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To establish a rapid detection method of African swine fever virus (ASFV) DNA based on enzymatic recombinase amplification (ERA) technology.

Method
Specific ERA probes and primers were designed according to the conserved sequences of B646L and EP402R genes, and MGF360/505 gene which was one of the ASFV multigene family members. Through optimizing reaction conditions, the ERA method for detecting ASFV DNA was finally established under the isothermal condition of 42 ℃.

Result
The detection results of three methods of ERA-ASFV-B646L, ERA-ASFV-EP402R and ERA-ASFV-MGF could be obtained within 16, 7 and 13 min respectively. The copy number detection limitation of positive sample was all 10² μL⁻¹. The coincidence rate was 100% compared with the common method in the technical standard of ASFV diagnosis in China.

Conclusion
The established ERA method can be used for rapid detection of ASFV, and provides an effective rapid detection method for epidemiological investigation and field detection.
- African swine fever,
- enzymatic recombinase amplification,
- isothermal amplification,
- rapid detection

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References (16)

References

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